Mass Spectrometric and Glycan Microarray–Based Characterization of the Filarial Nematode Brugia malayi Glycome Reveals Anionic and Zwitterionic Glycan Antigens

Petralia, Laudine M. C.; Diepen, Angela van; Lokker, Lena A.; Nguyen, Dieu Linh; Sartono, Erliyani; Khatri, Vishal; Kalyanasundaram, Ramaswamy; Taron, Christopher H.; Foster, Jeremy M.; Hokke, Cornelis H.

doi:10.1016/j.mcpro.2022.100201

“…At the individual N-glycan level, changes arise early, as soon as 5 wpi, when none of the animals was yet microfilariaemic (Supplementary Table S3 online). This is consistent with immune responses to parasite (glycan) antigens already occurring at 5 wpi 74 . Surely, given their significant contribution to serum N-glycans 1 , induction of Igs to B. malayi antigens (BmA) may affect the overall profile.…”

Section: Discussionsupporting

confidence: 88%

“…Surely, given their significant contribution to serum N-glycans 1 , induction of Igs to B. malayi antigens (BmA) may affect the overall profile. In particular, specific IgM to parasite glycans has been observed to appear early during infection (at 5 wpi) and to decrease gradually thereafter 74 . This dynamic of early immune response might explain early changes in the N-glycoprofile ( e.g.…”

Section: Discussionmentioning

confidence: 99%

Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

Petralia

¹

,

Santha

²

,

Behrens

³

et al. 2022

Sci Rep

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Serum N-glycan profiling studies during the past decades have shown robust associations between N-glycan changes and various biological conditions, including infections, in humans. Similar studies are scarcer for other mammals, despite the tremendous potential of serum N-glycans as biomarkers for infectious diseases in animal models of human disease and in the veterinary context. To expand the knowledge of serum N-glycan profiles in important mammalian model systems, in this study, we combined MALDI-TOF-MS analysis and HILIC-UPLC profiling of released N-glycans together with glycosidase treatments to characterize the glycan structures present in rhesus macaque serum. We used this baseline to monitor changes in serum N-glycans during infection with Brugia malayi, a parasitic nematode of humans responsible for lymphatic filariasis, in a longitudinal cohort of infected rhesus macaques. Alterations of the HILIC-UPLC profile, notably of abundant structures, became evident as early as 5 weeks post-infection. Given its prominent role in the immune response, contribution of immunoglobulin G to serum N-glycans was investigated. Finally, comparison with similar N-glycan profiling performed during infection with the dog heartworm Dirofilaria immitis suggests that many changes observed in rhesus macaque serum N-glycans are specific for lymphatic filariasis.

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“…S3 online). This is consistent with immune responses to parasite (glycan) antigens already occurring at 5 wpi [74]. Surely, given their signi cant contribution to serum Nglycans [1], induction of Igs to B. malayi antigens (BmA) may affect the overall pro le.…”

Section: Discussionsupporting

confidence: 74%

“…Surely, given their signi cant contribution to serum Nglycans [1], induction of Igs to B. malayi antigens (BmA) may affect the overall pro le. In particular, speci c IgM to parasite glycans has been observed to appear early during infection (at 5 wpi) and to decrease gradually thereafter [74]. This dynamic of early immune response might explain early changes in the N-glycopro le (e.g.…”

Section: Discussionmentioning

confidence: 95%

Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

Petralia

¹

,

Santha

²

,

Behrens

³

et al. 2022

Preprint

Self Cite

0

View full text Add to dashboard Cite

Serum N-glycan profiling studies during the past decades have shown robust associations between N-glycan changes and various biological conditions, including infections, in humans. Similar studies are scarcer for other mammals, despite the tremendous potential of serum N-glycans as biomarkers for infectious diseases in animal models of human disease and in the veterinary context. To expand the knowledge of serum N-glycan profiles in important mammalian model systems, in this study, we combined MALDI-TOF-MS analysis and HILIC-UPLC profiling of released N-glycans together with glycosidase treatments to characterize the glycan structures present in rhesus macaque serum. We used this baseline to monitor changes in serum N-glycans during infection with Brugia malayi, a parasitic nematode of humans responsible for lymphatic filariasis, in a longitudinal cohort of infected rhesus macaques. Alterations of the HILIC-UPLC profile, notably of abundant structures, became evident as early as 5 weeks post-infection. Given its prominent role in the immune response, contribution of immunoglobulin G to serum N-glycans was investigated. Finally, comparison with similar N-glycan profiling performed during infection with the dog heartworm Dirofilaria immitis suggests that many changes observed in rhesus macaque serum N-glycans are specific for lymphatic filariasis.

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“…They reported a total of 291 N-glycosylated proteins occurring primarily in the intestine and gonads. In the same direction, Petralia and colleagues [ 33 ] investigated the glycans expressed by the filarial nematode Brugia malayi at different stages of development (microfilariae, third-stage larvae (L3), and adult worms (males and females)) using MALDI-TOF MS combined with glycan sequencing, tandem mass spectrometry (MS/MS) and glycan microarrays. They identified several antigen motifs such as phosphorylcholine and terminal glucuronic acid.…”

Section: Specific Application Of Maldi-tof Ms For Different Helminthsmentioning

confidence: 99%

Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths

Sy

¹

,

Conrad

²

,

Becker

³

2022

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Helminth infections caused by nematodes, trematodes, and cestodes are major neglected tropical diseases and of great medical and veterinary relevance. At present, diagnosis of helminthic diseases is mainly based on microscopic observation of different parasite stages, but microscopy is associated with limited diagnostic accuracy. Against this background, recent studies described matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry as a potential, innovative tool for helminth identification and differentiation. MALDI-TOF mass spectrometry is based on the analysis of spectra profiles generated from protein extracts of a given pathogen. It requires an available spectra database containing reference spectra, also called main spectra profiles (MSPs), which are generated from well characterized specimens. At present, however, there are no commercially available databases for helminth identification using this approach. In this narrative review, we summarize recent developments and published studies between January 2019 and September 2022 that report on the use of MALDI-TOF mass spectrometry for helminths. Current challenges and future research needs are identified and briefly discussed.

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Mass Spectrometric and Glycan Microarray–Based Characterization of the Filarial Nematode Brugia malayi Glycome Reveals Anionic and Zwitterionic Glycan Antigens

Cited by 20 publications

References 98 publications

Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths

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