Mass spectrometric characterization of flavonoids in extracts from Passiflora incarnata

Raffaelli, Andrea; Moneti, Gloriano; Mercati, V.; Toja, E.

doi:10.1016/s0021-9673(97)00260-4

Cited by 68 publications

(41 citation statements)

References 9 publications

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“…MS/MS in the negative ion mode using an ion trap instrument, 11 and CID using ion trap, 12 triple quadrupole 13 or magnetic sector 14 spectrometers all revealed differences in the fragmentation patterns between schaftoside and isoschaftoside sufficient to allow their differentiation and a positive ion method using FAB-CID has also been described. 15 According to such reports, 11 preferential fragmentation is of the sugar moiety at the 6-C rather than the 8-C position.…”

Section: 8-di-c-glycosylflavonesmentioning

confidence: 99%

Study of C- and O-glycosylflavones in sugarcane extracts using liquid chromatography: exact mass measurement mass spectrometry

Colombo¹,

Yariwake²,

McCullagh³

2008

J. Braz. Chem. Soc.

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Os flavonóides presentes nos extratos de cana-de-açúcar (Saccharum officinarum) foram analisados por cromatografia líquida acoplada à espectrometria de massas (LC-MS), e o estudo da rota de fragmentação dos flavonóides selecionados foi realizado utilizando a espectrometria de massas com aceleração ortogonal por tempo de vôo e ionização eletrospray (ESI-oa-ToF MS). Sete flavonas C-e O-glicosiladas foram identificadas nos extratos: schaftosídeo, isoschaftosídeo, luteolina-8-C-(ramnosilglicosídeo), vitexina, orientina, tricina-7-O-neohesperidosídeo e tricina-7-O-glicosídeo. Destas, cinco foram identificadas sem comparação direta com seus respectivos padrões. O método descrito também permitiu a diferenciação das 6-C e 8-C flavonas isoméricas, schaftosídeo e isoschaftosídeo. A combinação dos dados de fragmentação e a medida de massa exata mostraram ser complementares às técnicas de HPLC-UV-MS previamente utilizadas para discriminação de isômeros nos estudos da cana-de-açúcar.The flavonoids present in sugarcane (Saccharum officinarum) extracts were analyzed by liquid chromatography -mass spectrometry (LC-MS), and a study of the fragmentation patterns of selected flavonoids was conducted using orthogonal acceleration time-of-flight electrospray ionization mass spectrometry (ESI-oa-ToF MS). Seven C-and O-glycosylflavones were identified in the extracts, namely, schaftoside, isoschaftoside, luteolin-8-C-(rhamnosylglucoside), vitexin, orientin, tricin-7-O-neohesperidoside and tricin-7-O-glucoside. Of these, five were identified in the absence of direct comparison with their respective standards. The described method also permitted the differentiation of the 6-C and 8-C isomeric flavones, schaftoside and isoschaftoside. The combination of fragmentation data and exact mass measurement showed to be complimentary to the HPLC-UV-MS techniques previously utilized for isomers discrimination in sugarcane studies.

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Section: 8-di-c-glycosylflavonesmentioning

confidence: 99%

Study of C- and O-glycosylflavones in sugarcane extracts using liquid chromatography: exact mass measurement mass spectrometry

Colombo¹,

Yariwake²,

McCullagh³

2008

J. Braz. Chem. Soc.

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“…Suas aplicações clínicas no mundo inteiro são evidentes pelo fato da P. incarnata ser uma planta oficial na British Herbal Pharmacopoeia 1983, Homoeopathic Pharmacopoeia da (Dhawan et al, 2001b). Os flavonóides são relatados como sendo os fitoconstituintes mais abundantes encontrados nessa espécie (Raffaelli et al, 1997). Passiflora incarnata é usada em diversas preparações farmacêuticas, em combinação com outras drogas (Tankanow et al, 2003).…”

Section: Introductionunclassified

Estudo de toxicologia clínica de um fitoterápico contendo Passiflora incarnata L., Crataegus oxyacantha L., Salix alba L. em voluntários saudáveis

Nascimento¹,

Santana²,

Leite³

et al. 2009

Rev. bras. farmacogn.

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ArtigoEstudo de toxicologia clínica de um fitoterápico contendo Passiflora incarnata L., Crataegus oxyacantha L., Salix alba L. em voluntários saudáveis ABSTRACT: "Clinical toxicology study of an herbal medicine with Passiflora incarnata L., Crataegus oxyacantha L., Salix alba L. in healthy volunteers". Pasalix® is an herbal medicine containing a combination of three medicinal plants: Passiflora incarnata, Salix alba and Crataegus oxyacantha. Its main indication is to treat anxiety and insomnia. The purpose of this study was to evaluate the clinical toxicology of that formulation in healthy volunteers. For this, a nonrandomized open clinical trial was conducted with 24 healthy male volunteers, who received two (2) coated tablets of the herbal medicine twice a day for 28 uninterrupted days. The volunteers were included in the study only when considered healthy after clinical assessment, physical examination and laboratory tests which preceded the study. The laboratory tests included: hematological, biochemical and serological analysis. The clinical and laboratory evaluation was repeated after the 1 st , 2 nd , 3 rd and 4 th weeks of the treatment and 7 days after the last administration. Pasalix® was well tolerated by the 24 volunteers, and it has showed no serious adverse events. The clinical, laboratory, and electrocardiographic data assessed before, during and after the test showed no signals of toxicity in various organs and systems evaluated, confirming the safety of the preparation for use in trials of therapeutic efficacy.

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“…In vitro antioxidant activity has also been described for aqueous and ethanolic extracts of P. incarnata in the DPPH (2.2-Diphenyl-1-picrylhydrazide) and ABTS (Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) methods (Masteikova et al, 2008). Flavonoids have been described as the main phytoconstituents of P. incarnata (Raffaelli, Moneti, Toja, 1997) and the antioxidant activity of this species has been attributed to these molecules (Masteikova et al, 2008). Flavonoids are phenolic substances that can stabilize and delocalize the unpaired electron within its aromatic ring and are able to reduce free radical formation and to scavenge free radicals.…”

Section: Discussionmentioning

confidence: 99%

Passiflora incarnata treatment during gestation and lactation: toxicological and antioxidant evaluation in wistar dams

Boll

Bortolasci

Zaminelli

et al. 2014

Braz. J. Pharm. Sci.

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Passiflora incarnata is marketed in many countries as a phytomedicine. Even though the directions of most marketed phytomedicines recommend them to be used under medical supervision, reproductive and developmental studies are sparse and not mandatory for regulatory purposes. In this study, a reproductive toxicity evaluation of P. incarnata was conducted in Wistar rats gavaged (30 or 300 mg/kg) during pregnancy and lactation. Moreover, considering that antioxidant properties have been attributed to flavonoids present in the genus Passiflora, it was also evaluated the antioxidant/pro-oxidant balance in the plasma of these dams and the antioxidant potential in an in vitro test. P. incarnata treatment did not influence dams´ body weight as well as reproductive (gestation length, post-implantation loss, litter size, litter weight) and hepatic (albumin, AST, ALT, GGT) parameters. The antioxidant property of P. incarnata was evidenced both in vivo (increase in the total antioxidant plasmatic potential) and in vitro (decrease in neutrophil-induced respiratory burst). The results from the present study indicate that under the experimental conditions evaluated, P. incarnata treatment during gestation and lactation presented antioxidant activity in the absence of maternal reproductive toxicity.Uniterms: Phytomedicines/evaluation. Passiflora incarnata/use in pregnancy. Passiflora incarnata/ use in lactation. Passiflora incarnata/reproductive toxicity/experimental study. Pregnancy/use of phytomedicines. Lactation/use of phytomedicines. Oxidative stress.Passiflora incarnata é comercializada em muitos países como fitoterápico. Embora a bula da maioria dos fitoterápicos recomende que eles sejam usados sob supervisão médica, estudos sobre a toxicidade reprodutiva e do desenvolvimento desses produtos são raros e não obrigatórios para fins regulatórios. Neste estudo, realizamos uma avaliação da toxicidade reprodutiva da P. incarnata, administrada a ratas Wistar (30 ou 300 mg/kg, gavagem) durante a gestação e a lactação. Além disso, considerando as propriedades antioxidantes que têm sido atribuídas aos flavonoides presentes no gênero Passiflora, também avaliou-se o equilíbrio antioxidante/pró-oxidante no plasma destas fêmeas e conduziu-se um teste in vitro para avaliar o potencial antioxidante. O tratamento com P. incarnata não influenciou o peso corporal das fêmeas, bem como indicadores de toxicidade reprodutiva (perdas pós-implantação, número de filhotes vivos e peso da ninhada) e os parâmetros de função hepática (albumina, AST, ALT, GGT). A propriedade antioxidante da P. incarnata foi evidenciada tanto in vivo (aumento do potencial antioxidante total plasmático) quanto in vitro (diminuição do burst respiratório em neutrófilos). Os resultados deste estudo indicam que, nas condições experimentais avaliadas, o tratamento com P. incarnata durante a gestação e lactação apresentou efeito antioxidante, na ausência de toxicidade reprodutiva materna.Unitermos: Fitoterápicos/avaliação. Passiflora incarnata/uso na gestação. Passiflora inca...

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Mass spectrometric characterization of flavonoids in extracts from Passiflora incarnata

Cited by 68 publications

References 9 publications

Study of C- and O-glycosylflavones in sugarcane extracts using liquid chromatography: exact mass measurement mass spectrometry

Study of C- and O-glycosylflavones in sugarcane extracts using liquid chromatography: exact mass measurement mass spectrometry

Estudo de toxicologia clínica de um fitoterápico contendo Passiflora incarnata L., Crataegus oxyacantha L., Salix alba L. em voluntários saudáveis

Passiflora incarnata treatment during gestation and lactation: toxicological and antioxidant evaluation in wistar dams

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