Mass spectrometric investigations to obtain the first direct comparisons of endogenous breath and blood volatile organic compound concentrations in healthy volunteers

O’Hara, Margaret; Clutton-Brock, Tom; Green, Stuart; O'Hehir, Shane; Mayhew, Chris A.

doi:10.1016/j.ijms.2008.12.005

Cited by 25 publications

(26 citation statements)

References 33 publications

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“…This was manifested by a rapid increase of acetone levels during the breath and skin emission phase of the experiment. Acetone is the major VOC produced in the human organism exhibiting high abundances in breath [15,27], blood [28], and urine [29,30]. Several sources of acetone in human body can be listed.…”

Section: Resultsmentioning

confidence: 99%

Monitoring of selected skin- and breath-borne volatile organic compounds emitted from the human body using gas chromatography ion mobility spectrometry (GC-IMS)

Mochalski

Wiesenhofer

Allers

et al. 2018

Journal of Chromatography B

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Human smuggling and associated cross-border crimes have evolved as a major challenge for the European Union in recent years. Of particular concern is the increasing trend of smuggling migrants hidden inside shipping containers or trucks. Therefore, there is a growing demand for portable security devices for the non-intrusive and rapid monitoring of containers to detect people hiding inside. In this context, chemical analysis of volatiles organic compounds (VOCs) emitted from the human body is proposed as a locating tool. In the present study, an in-house made ion mobility spectrometer coupled with gas chromatography (GC-IMS) was used to monitor the volatile moieties released from the human body under conditions that mimic entrapment. A total of 17 omnipresent volatile compounds were identified and quantified from 35 ion mobility peaks corresponding to human presence. These are 7 aldehydes (acrolein, 2-methylpropanal, 3-methylbutanal, 2-ethacrolein, n-hexanal, n-heptanal, benzaldehyde), 3 ketones (acetone, 2-pentanone, 4-methyl-2-pentanone), 5 esters (ethyl formate, ethyl propionate, vinyl butyrate, butyl acetate, ethyl isovalerate), one alcohol (2-methyl-1-propanol) and one organic acid (acetic acid). The limits of detection (0.05-7.2 ppb) and relative standard deviations (0.6-11%) should be sufficient for detecting these markers of human presence in field conditions. This study shows that GC-IMS can be used as a portable field detector of hidden or entrapped people.

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Section: Resultsmentioning

confidence: 99%

Monitoring of selected skin- and breath-borne volatile organic compounds emitted from the human body using gas chromatography ion mobility spectrometry (GC-IMS)

Mochalski

Wiesenhofer

Allers

et al. 2018

Journal of Chromatography B

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“…If dissociative proton transfer occurs then it is not extensive in terms of the number of resulting product ions. Operational parameters used for this investigation were those previously reported ( O'Hara et al, 2009a,b ). Namely, the drift-tube was maintained at a pressure of 2.07 ± 0.01 mbar and temperature of 45 ± 1 °C.…”

Section: Methodsmentioning

confidence: 99%

Volatile Biomarkers in Breath Associated With Liver Cirrhosis — Comparisons of Pre- and Post-liver Transplant Breath Samples

Río

O’Hara

Holt³

et al. 2015

EBioMedicine

112

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BackgroundThe burden of liver disease in the UK has risen dramatically and there is a need for improved diagnostics.AimsTo determine which breath volatiles are associated with the cirrhotic liver and hence diagnostically useful.MethodsA two-stage biomarker discovery procedure was used. Alveolar breath samples of 31 patients with cirrhosis and 30 healthy controls were mass spectrometrically analysed and compared (stage 1). 12 of these patients had their breath analysed after liver transplant (stage 2). Five patients were followed longitudinally as in-patients in the post-transplant period.ResultsSeven volatiles were elevated in the breath of patients versus controls. Of these, five showed statistically significant decrease post-transplant: limonene, methanol, 2-pentanone, 2-butanone and carbon disulfide. On an individual basis limonene has the best diagnostic capability (the area under a receiver operating characteristic curve (AUROC) is 0.91), but this is improved by combining methanol, 2-pentanone and limonene (AUROC curve 0.95). Following transplant, limonene shows wash-out characteristics.ConclusionsLimonene, methanol and 2-pentanone are breath markers for a cirrhotic liver. This study raises the potential to investigate these volatiles as markers for early-stage liver disease. By monitoring the wash-out of limonene following transplant, graft liver function can be non-invasively assessed.

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Section: —Advantages and Disadvantages Of Biological Specimen Collectmentioning

confidence: 99%

“…Published research on VOC detection and identification on different biological specimens generally focuses on only one specimen. Research on VOC detection across multiple specimens is usually only limited to two, at most three specimens (1,10,27,28). Statheropoulos et al reported the use of VOCs from human expired air, blood, and urine for locating entrapped people in earthquakes to determine whether a relatively small target group of VOCs common to all three matrices can be determined (29).…”

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confidence: 99%

Comparison of the Volatile Organic Compounds from Different Biological Specimens for Profiling Potential*

Kusano

Mendez

Furton

2012

Journal of Forensic Sciences

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: Previous work has demonstrated the ability to differentiate individuals based on the analysis of human scent hand odor chemicals. In this paper, a range of forensic biological specimens are shown to also have the ability to differentiate individuals based upon the volatile organic compounds (VOCs) present. Human VOC profiles from hand odor, oral fluid, breath, blood, and urine of 31 individuals were analyzed by solid‐phase microextraction–gas chromatography–mass spectrometry (SPME‐GC‐MS) and combined methods of chromatogram comparison, Spearman rank correlation comparison, and principal component analysis. Intra‐specimen comparisons demonstrated the distinguishability of individuals above 99%. Inter‐specimen VOC profiles from the same individual were found to be too different to be used for scent‐matching purposes, with Spearman rank coefficients below 0.15. A 6‐month VOC profile monitoring of two individuals demonstrated the consistency of VOC profiles over time across specimens.

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Mass spectrometric investigations to obtain the first direct comparisons of endogenous breath and blood volatile organic compound concentrations in healthy volunteers

Cited by 25 publications

References 33 publications

Monitoring of selected skin- and breath-borne volatile organic compounds emitted from the human body using gas chromatography ion mobility spectrometry (GC-IMS)

Monitoring of selected skin- and breath-borne volatile organic compounds emitted from the human body using gas chromatography ion mobility spectrometry (GC-IMS)

Volatile Biomarkers in Breath Associated With Liver Cirrhosis — Comparisons of Pre- and Post-liver Transplant Breath Samples

Comparison of the Volatile Organic Compounds from Different Biological Specimens for Profiling Potential*

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