Mass spectrometric profiling and pattern recognition

Taş, A. Cüneyt; Greef, J. van der

doi:10.1002/mas.1280130204

Cited by 16 publications

(12 citation statements)

References 125 publications

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“…Projections of individual sample spectra on these functions reveal the main characteristics of the spectra compared to the origin and thus give information on the molecular composition of the samples. Details on the procedure can be found elsewhere (Hoogerbrugge et al 1983, Windig et al 1983, Tas 1991. Klap 1997.…”

Section: Methodsmentioning

confidence: 99%

Retention of lignin in seagrasses:angiosperms that returned to the sea

Klap¹,

Hemminga²,

Boon³

2000

Mar. Ecol. Prog. Ser.

101

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Using Curie-point Pyrolysis Gas Chromatography Mass Spectrometry [Py-GCMS) and Direct Temperature-resolved Mass Spectrometry (DT-bfS), lignin was detected In highly purified preparations (Milled Wood Lignin = hlWL) of various tissues of the seagrasses Zostera rnarina and Posidonia oceanica. The results ind~cate that P. oceanica contains more lign~n than Z. manna and that roots and rhizomes generally contain more Lignin than leaves. It is concluded that the ability to produce lignin is not lost by the angiosperm ancestors of extant seagrasses upon their colonization of the marine environment. Relative lignin abundances in the different tissues appear to be posltlvely correlated with life span. It is suggested that lignification contributes to the longevity of a tissue by protecting lt against microbial attack, but that deposition of lignin in seagrasses is restricted to tissues that show limited growth.

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Section: Methodsmentioning

confidence: 99%

Retention of lignin in seagrasses:angiosperms that returned to the sea

Klap¹,

Hemminga²,

Boon³

2000

Mar. Ecol. Prog. Ser.

101

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“…(Horning and Horning, 1971;Jellum et al, 1981;Greenaway et al, 1991;Tas and van der Greef, 1994)), the basics of metabolomics studies (as scientific studies) are not, and they can thus benefit greatly from the accumulated knowledge and standards that have become the norm in mainstream biology and medicine.…”

Section: Introduction: Binary Class Discrimination Problemsmentioning

confidence: 99%

Statistical strategies for avoiding false discoveries in metabolomics and related experiments

2006

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“…Differences are expressed as factor scores or (when analyzed in multiplicate) discriminant scores. Details on the procedure can be found elsewhere (Hoogerbrugge et al 1983;Windig et al 1983;Boon et al 1984;Tas 1991;Klap 1997). Figure 5 shows two average spectra, in this case the results obtained after EI (Fig.…”

Section: Resultsmentioning

confidence: 99%

Decomposition dynamics of six salt marsh halophytes as determined by cupric oxide oxidation and direct temperature‐resolved mass spectrometry

Klap

Louchouarn

Boon

et al. 1999

Limnology & Oceanography

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This paper presents the results of a comparative study on the aerobic decomposition of six salt marsh plant species over a period of 2 yr. In addition to ash‐free dry weight (AFDW) determination and elemental analysis (C and N), two analytic methods have been applied to obtain insight into the decomposition dynamics of lignin in the various plant tissues. The analytic methods are (1) cupric oxide (CuO) oxidation followed by gas chromatography—mass spectrometry (GC‐MS) and (2) direct temperature‐resolved mass spectrometry (DT‐MS). AFDW losses could generally be well described by double exponential relations with time. Carbon‐to‐nitrogen ratios increased during the initial stages of decomposition and decreased again afterward. For five of the six plant species, a correlation between initial lignin content and AFDW loss was observed. Decay dynamics of lignin denoted a rapid relative increase during the first weeks of field exposure, followed by stabilizing contents over the next 2 yr. CuO oxidation data indicate the establishment of a stable “lignin endmember” within 1–2 months. DT‐MS data, on the contrary, showed continuous modification of the lignin polymer throughout the duration of the experiment. Evidence was found for the incorporation of (presumably) microbial N‐acetylglucosamine in the complex residue produced upon decomposition. Combination of CuO oxidation and DT‐MS data suggested that lignin degradation products became attached to the original macromolecular material and could still be identified as lignin‐derived material. The data support a humification mechanism via condensation of small degradation products instead of the selective preservation of certain biomacromolecules (like lignin).

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Mass spectrometric profiling and pattern recognition

Cited by 16 publications

References 125 publications

Retention of lignin in seagrasses:angiosperms that returned to the sea

Retention of lignin in seagrasses:angiosperms that returned to the sea

Statistical strategies for avoiding false discoveries in metabolomics and related experiments

Decomposition dynamics of six salt marsh halophytes as determined by cupric oxide oxidation and direct temperature‐resolved mass spectrometry

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