Mass spectrometric profiling of cord blood serum proteomes to distinguish infants with intrauterine growth restriction from those who are small for gestational age and from control individuals

Apolipoprotein E Genotype in Very Preterm Neonates with Intrauterine Growth Restriction: An Analysis of the German Neonatal Network Cohort

“…We took advantage of a nation-wide genomic study of the German Neonatal Network (GNN) including more than 18000 preterm neonates born before 32 weeks of gestation or with very low birth weight below 1500 g. The neonates were clustered according to birth weight percentiles with the lowest percentiles likely reflecting impaired fetal development. However, IUGR is not uniquely defined and neonates can be born small for their gestational age (SGA) by genetic determination and in the absence of a pathologic process [5, 22]. Hence, in this study different SGA subtypes and scenarios have been considered including different cut-offs of percentiles to define SGA as well as factors associated with IUGR like hypertensive disorders in pregnancy.…”

Alterations of protein expression in serum of infants with intrauterine growth restriction and different gestational ages

“…In consequence, most proteins identified were lipoprotein components and transporters, and major blood proteins, as several hemoglobin chains, were also included [26]. Subsequently, these authors reported that MS profiling is confirmatory to clinical surveillance with the potential to identify neonates with IUGR postnatally [27]. As far as we know, the present study is the first to assess the changes in serum proteome of IUGR neonates stratified by their GA, in venous blood samples drawn at 1, 7 and 30 days after birth, not in umbilical cord blood as previously made in previous studies in human neonates [24][25][26][27].…”

“…Subsequently, these authors reported that MS profiling is confirmatory to clinical surveillance with the potential to identify neonates with IUGR postnatally [27]. As far as we know, the present study is the first to assess the changes in serum proteome of IUGR neonates stratified by their GA, in venous blood samples drawn at 1, 7 and 30 days after birth, not in umbilical cord blood as previously made in previous studies in human neonates [24][25][26][27]. Sera from each GA group were pooled, so that each neonate contributed an equal amount of protein to the mixture before 2-DE analysis.…”

“…Sample pooling has become a common practice in Proteomics, because the analysis of pooled samples helps to reduce the number of replicates required and the standard deviation obtained from individual samples [35]. To focus on minority serum proteins and to avoid any bias toward lipoproteins [26,27,34], the abundant serum proteins were depleted using the ProteoMiner TM kit (Bio-Rad®), which depletes high-abundance proteins via binding to a highly diverse bead-based library of combinatorial peptides while enriching those of medium-and low-abundance, without using immunodepletion.…”

“…In umbilical cord blood sera of 15 IUGR and 15 AGA newborns fractionated by hydrophobic interactions, the six best differentiating ion signals obtained by MALDI-TOF profiling were assigned as IUGR proteome signature, proposing apolipoprotein C-III 0 as potential key marker [26]. Mass spectrometric profiling is confirmatory to clinical surveillance with the potential to identify neonates with IUGR postnatally [27].…”