Mass-spectrometric study of carbohydrates

Chizhov, O. S.; Golovkina, L. S.; Wulfson, N.S.

doi:10.1016/s0008-6215(00)81503-7

Cited by 37 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mass spectra were recorded at an ionisation potential of 70 eV and an ionisation current of 300 pA and an ion source temperature of 100 "C. For sugar analysis the oligosaccharide and D-xylose as internal standard were hydrolysed and the resulting sugars were converted into alditol acetates and analysed by gas-liquid chromatography -mass spectrometry [16,17]. The following conditions were used in the hydrolysis step: 0.25 M H2S0,, lOO"C, 18 h (A); 4 M HC1,lOO "C, 4 h (B); 90 HCOOH, 100 "C,…”

Section: General Methodsmentioning

confidence: 99%

A Urinary Pentasaccharide in Bovine Mannosidosis

Lundblad¹,

Nilsson²,

Nordén³

et al. 1975

European Journal of Biochemistry

View full text Add to dashboard Cite

Abnormally high amounts of low molecular weight mannose-rich carbohydrate material were found in the urine of an Angus calf with mannosidosis. At least five oligosaccharide fractions were detected by paper chromatography. The most abundant compound was purified by gel chromatography, zone electrophoresis, and two consecutive preparative paper chromatographic steps. The yield was 10 mg/liter of urine. From structural studies including nuclear magnetic resonance spectroscopy, optical rotation, sugar analysis, methylation analysis, and partial enzymatic degradation the following structure was deduced :This oligosaccharide is distinct from all the oligosaccharides previously described which are excreted by patients with mannosidosis.Bovine mannosidosis, previously known as pseudolipidosis [l], is caused by a deficiency of lysosomal a-mannosidase [2]. The enzymatic defect [3] is analogous to the defect in human mannosidosis. Accumulation of mannose-containing oligosaccharides has been demonstrated in human brain tissue [4], bovine lymph nodes [2], and bovine brains [ 5 ] , but none of these compounds was isolated in amount sufficient for a complete structural characterization. Urine from patients with mannosidosis is a rich source of several mannose-containing oligosaccharides and three of these have been isolated and characterized [6,7]. The brain oligosaccharides isolated from bovine mannosidosis were shown by paper chromatography to be distinct from the urine oligosaccharides from the human form of the disease [5]. We now report the complete structure of the most predominant oligosaccharide from the urine of a case of bovine mannosidosis. This oligosaccharide is structurally different from all the human mannosidosis urinary oligosaccharides previously described. MATERIALS AND METHODS MaterialsUrine (7.5 1) was collected from a New Zealand calf with mannosidosis. The calf was a 10-month-old Abhreviaiions. Manp, mannopyranoside; GlcNAcp, 2-acetamido-2-deoxy-~-glucopyranoside; GlcNAc, 2-acetamido-2-deoxy-D-glUCOSe.Enzyme. a-Mannosidase (EC 3.2

show abstract

Section: General Methodsmentioning

confidence: 99%

A Urinary Pentasaccharide in Bovine Mannosidosis

Lundblad¹,

Nilsson²,

Nordén³

et al. 1975

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…The results show that these sugars follow basically the same fragmentation routes as their aminosugar analogues. However, the sugar derivatives (41-47) also show the fragmentation designated as hfragmentation, 13 which produces the fragments h 1 (m/z 149) and h 2 ([M -149]). This fragmentation is not important in the case of the N-alkyl and N,N-dialkyl aminosugar derivatives (Scheme 3).…”

Section: →]mentioning

confidence: 99%

The C3H6O+• fragment from 1,4-dioxane+• revisited

Vega‐Pérez

Candela

Alcudia

et al. 1999

Eur. J. Mass Spectrom.

View full text Add to dashboard Cite

The electron impact spectra of 2-N-alkyl, 2-N-acyl-2-N-alkyl and 2-N,N-dialkyl aminosugar derivatives, as well as 2-aminosugar derivatives themselves, all show an [H 1 2 + 13] + ion. b In previous work, it was considered that this ion was derived from the [M-OR 1 ] + ion and was designated as [M-OR 1-178] +. In the present work, the structure and origin of the [H 1 2 + 13] + ion is studied in detail, and it is demonstrated that it originates first from the molecular ion, via a primary fragmentation, and second from the [M-OR 1 ] + ion, in both cases by a [3 + 3] cleavage of the pyranose ring.

show abstract

“…Studies on the ionization and dissociation of cyclic ethers (343) and the scission probabilities of the skeletal bonds of aliphatic ethers (344) are of theoretical interest. A new fragmentation reaction was reported from the mass spectra of cyclic acetals of alditols that contain the 1,3,6,8-tetraoxabicy clo-[4.4.0]decane ring system (345). The mass spectra of 5,6-dihydro--pyran derivatives (346), of alkyl tetrahydropyranyl ethers and thioethers (347), of some cyclic ethers (348), and of triphenylmethyl ethers (849) have been discussed.…”

Section: The Factors Influencing [A+]/[m+]mentioning

confidence: 99%

Mass spectrometry

DeJongh¹

1970

Anal. Chem.

View full text Add to dashboard Cite

Mass-spectrometric study of carbohydrates

Cited by 37 publications

References 7 publications

A Urinary Pentasaccharide in Bovine Mannosidosis

A Urinary Pentasaccharide in Bovine Mannosidosis

The C3H6O+• fragment from 1,4-dioxane+• revisited

Mass spectrometry

Contact Info

Product

Resources

About