Mass spectrometry analysis and in silico prediction of allergenicity of peptides in tryptic hydrolysates of the proteins from Ruditapes philippinarum

Abstract

The abundant peptide information contributes to further investigations of the structure and potential function of R. philippinarum. Additional in vitro studies are required to demonstrate and ensure the correct production of the hydrolysates for use in the food industry with respect to R. philippinarum. © 2017 Society of Chemical Industry.

Identification and characterization of a calcium-binding peptide from salmon bone for the targeted inhibition of α-amylase in digestion

“…The number of missed cleavage sites was 2, and the enzyme digestion mode was Trypsin/P. The screened peptides were characterized by HPLC as well as ESI-MS ( Yu et al, 2017 ).…”

Development and Validation of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Quantitative Determination of N-((3S,4S)-4-(3,4-Difluorophenyl)piperidin-3-yl)-2-fluoro-4-(1-methyl-1H-pyrazol-5-yl)benzamide in Dog Plasma

“…Biological sample preparation can be achieved using various methods, which mainly include protein precipitation [13,14], enzymatic hydrolysis [15], and extraction [16,17]. Given low in vivo concentrations of analytes, sample preparation methods should maximally eliminate the interference of impurities to ensure that the recovery of each component is at an acceptable range.…”