Mass spectrometry-based metabolomic discrimination of Cercospora leaf spot resistant and susceptible sugar beet germplasms

Soybean Metabolomics Based in Mass Spectrometry: Decoding the Plant’s Signaling and Defense Responses under Biotic Stress

“…LC–MS is an analytical system that offers robustness, sensitivity, and selectivity. The technique allows for the analysis of polar and nonpolar chemical compounds without derivatization steps, covering the primary and secondary metabolites involved in plant biotic stress, and it is frequently investigated in metabolomics studies. ,,,, …”

“…The detection of metabolites can be done in different mass analyzers, including quadrupole (Q), time of flight (TOF), triple quadrupole (QqQ), quadrupole time of flight (QTOF), ion trap (IT), linear trap quadrupole (LTQ), Orbitrap, and Fourier transform ion cyclotron resonance (FT-ICR), which can be used depending upon the sensitivity, resolution, and required dynamic mass range. QTOF is the most required analyzer for the study of plant metabolomics. ,,,,, The serial coupling of the two analyzers has a high resolution when used in reflectron mode, good sensitivity, and high data acquisition speed, allowing for the performance of sequential experiments (MS/MS). This analyzer can be used to discriminate data for the metabolite identification process, which ensures a better confirmation of the proposed structural identity …”

“…The technique allows for the analysis of polar and nonpolar chemical compounds without derivatization steps, covering the primary and secondary metabolites involved in plant biotic stress, and it is frequently investigated in metabolomics studies. 32,36,52,56,65 The LC−MS coupling has a disadvantage in relation to GC−MS regarding the structural identification process of the compounds through the use of standard libraries as a result of the variation of the retention time from run to run, even in the same equipment. The comparison of the training time retention can only be carried out under identical experimental conditions.…”

“…QTOF is the most required analyzer for the study of plant metabolomics. 32,36,52,56,68,69 The serial coupling of the two analyzers has a high resolution when used in reflectron mode, good sensitivity, and high data acquisition speed, 70 allowing for the performance of sequential experiments (MS/MS). This analyzer can be used to discriminate data for the metabolite identification process, which ensures a better confirmation of the proposed structural identity.…”

Bacterial Endophytes As Indicators of Susceptibility To Cercospora Leaf Spot (CLS) Disease In Beta vulgaris L.

“…Each plot consisted of an additional 287third row sown with a hybrid susceptible to CLS to facilitate the natural fungal infection in 288 the field. The scoring of CLS was performed per plot during the growing season of the plants 289 using a scale from 1 (no infection) to 9 (entire defoliation)[32,33]. The same described 290 genotypes and plant material were collected before the onset of CLS infection on June 12, 291 2019 and June 14, 2021 and under CLS infection on August 8, 2019, and August 9, 2021 for a comparative analysis.…”