Mass spectrometry‐based retina proteomics

Prókai, László; Zaman, Khadiza; Prókai-Tátrai, Katalin

doi:10.1002/mas.21786

Cited by 6 publications

(2 citation statements)

References 272 publications

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“…The method based on protein–small molecule interaction has been successfully applied to screening ligands for various soluble drug target proteins. It is closely integrated with biochemical and cell function experiments, demonstrating high potential as a tool for discovering novel lead compounds and revealing novel targets within key signal transduction pathways [ 89 ]. Affinity mass spectrometry can efficiently and rapidly screen 28 potential ligands from a pool of 20,000 compounds, leading to the discovery of three new adenosine A2A receptor antagonists [ 90 ].…”

Section: Gpcrs Help In the Discovery Of New Targeted Tcm Or Drugsmentioning

confidence: 99%

G protein-coupled receptors and traditional Chinese medicine: new thinks for the development of traditional Chinese medicine

Zhang,

An,

You

et al. 2024

Chin Med

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G protein-coupled receptors (GPCRs) widely exist in vivo and participate in many physiological processes, thus emerging as important targets for drug development. Approximately 30% of the Food and Drug Administration (FDA)-approved drugs target GPCRs. To date, the ‘one disease, one target, one molecule’ strategy no longer meets the demands of drug development. Meanwhile, small-molecule drugs account for 60% of FDA-approved drugs. Traditional Chinese medicine (TCM) has garnered widespread attention for its unique theoretical system and treatment methods. TCM involves multiple components, targets and pathways. Centered on GPCRs and TCM, this paper discusses the similarities and differences between TCM and GPCRs from the perspectives of syndrome of TCM, the consistency of TCM’s multi-component and multi-target approaches and the potential of GPCRs and TCM in the development of novel drugs. A novel strategy, ‘simultaneous screening of drugs and targets’, was proposed and applied to the study of GPCRs. We combine GPCRs with TCM to facilitate the modernisation of TCM, provide valuable insights into the rational application of TCM and facilitate the research and development of novel drugs. This study offers theoretical support for the modernisation of TCM and introduces novel ideas for development of safe and effective drugs.

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Section: Gpcrs Help In the Discovery Of New Targeted Tcm Or Drugsmentioning

confidence: 99%

G protein-coupled receptors and traditional Chinese medicine: new thinks for the development of traditional Chinese medicine

Zhang,

An,

You

et al. 2024

Chin Med

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“…However, mechanisms of glaucomatous neurodegeneration due to OHT have been poorly understood. While recent advances in mass spectrometry (MS)-based retina proteomics have been promising a better understanding of various retinal neuropathies [ 10 ], only a few proteomics studies have been published thus far with a focus on the glaucomatous retinae. Most of them analyzed tissues obtained from animal models of glaucoma, although the proteome of the diseased versus healthy human retinae has also been compared [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomics-Based Identification of Retinal Protein Networks Impacted by Elevated Intraocular Pressure in the Hypertonic Saline Injection Model of Experimental Glaucoma

Zaman

Nguyen

Prókai-Tátrai

et al. 2023

IJMS

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Elevated intraocular pressure is considered a major cause of glaucomatous retinal neurodegeneration. To facilitate a better understanding of the underlying molecular processes and mechanisms, we report a study focusing on alterations of the retina proteome by induced ocular hypertension in a rat model of the disease. Glaucomatous processes were modeled through sclerosing the aqueous outflow routes of the eyes by hypertonic saline injections into an episcleral vein. Mass spectrometry-based quantitative retina proteomics using a label-free shotgun methodology identified over 200 proteins significantly affected by ocular hypertension. Various facets of glaucomatous pathophysiology were revealed through the organization of the findings into protein interaction networks and by pathway analyses. Concentrating on retinal neurodegeneration as a characteristic process of the disease, elevated intraocular pressure-induced alterations in the expression of selected proteins were verified by targeted proteomics based on nanoflow liquid chromatography coupled with nano-electrospray ionization tandem mass spectrometry using the parallel reaction monitoring method of data acquisition. Acquired raw data are shared through deposition to the ProteomeXchange Consortium (PXD042729), making a retina proteomics dataset on the selected animal model of glaucoma available for the first time.

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