Mass Spectrometry–Driven Discovery of Neuropeptides Mediating Nictation Behavior of Nematodes

Cockx, Bram; Bael, Sven Van; Boelen, Rose; Vandewyer, Elke; Yang, Heeseung; Lê, Tuấn Anh; Dalzell, Johnathan J.; Beets, Isabel; Ludwig, Christina; Lee, Jun-Ho; Temmerman, Liesbet

doi:10.1016/j.mcpro.2022.100479

Cited by 8 publications

(3 citation statements)

References 89 publications

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“…Also, emerging evidence proposes that behavioral changes related to host finding may be regulated by neuropeptides. Neuropeptide characterization in S. carpocapsae was performed via sub-Dalton high-accuracy tandem mass spectrometry coupled with ultra-high performance liquid chromatography in order to know how EPNs regulate their behavior [96]. Such studies are essential to help boost EPN application and host specificity.…”

Section: Exploring Epns Molecular Tools For Favorable Plant-insect In...mentioning

confidence: 99%

Optimizing Entomopathogenic Nematode Genetics and Applications for the Integrated Management of Horticultural Pests

Abd-Elgawad

2023

Horticulturae

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Entomopathogenic nematodes (EPNs) can kill and recycle in their host populations, which bodes well for EPNs’ exploitation in long-term and safe pest management. However, EPNs’ cost and efficacy need transformational technology to supplant less expensive and more effective but toxic/unhealthy pesticides. A technology that allows for the significant uptake of commercial EPNs should both boost their market suitability and provide genetic improvements. This review provides brief overviews of EPNs’ biology and ecology from the standpoint of pest/pathogen management as a prerequisite for EPN improvements. Understanding the biology and ecology of EPNs, particularly their symbiotic relationships with bacteria, is crucial to their effective use in pest management. This review provides relevant insights into EPN-symbiotic bacteria and the EPN–symbiont complex. The symbiotic relationship between EPNs and bacteria plays a key role in IPM, providing unique advantages. Either of them can be included in mechanisms underlying the various positive sides of plant–insect interactions in emerging integrated pest management (IPM) systems. Recent approaches, in which EPNs can act additively or synergistically with other production inputs in IPM programs, are discussed for further expansion. The simultaneous favorable effects of EPNs and/or their mutualistic bacteria on several pest/pathogen species of crops should be identified. Merits, such as the rapid killing of insect pests, ease of EPN/the symbiont’s mass production and a broad host range, are presented in order to widely disseminate the conditions under which EPN usage can offer a cost-effective and/or value-added technique for IPM. To maximize the effectiveness of EPNs in IPM, various genetic improvement techniques are being explored. Such techniques, along with their merits/demerits and related tools, are reviewed to optimize the common biocontrol usage of EPNs. Examples of genetic improvements to EPNs that allow for their use in transformational technology, such as a cost-effective application technique, increased infectivity, and toleration of unfavorable settings, are given. Proper production practices and genetic techniques should be applied carefully to avoid undesirable results; it is suggested that these are considered on a case-by-case basis. This will enable us to optimize EPN performance based on the given variables.

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Section: Exploring Epns Molecular Tools For Favorable Plant-insect In...mentioning

confidence: 99%

Optimizing Entomopathogenic Nematode Genetics and Applications for the Integrated Management of Horticultural Pests

Abd-Elgawad

2023

Horticulturae

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“… 7 , 8 In general, neuropeptides typically activate specific neuropeptide receptors to regulate distinct behaviors. Interestingly, the capacity of a single neuropeptide receptor to be activated by multiple neuropeptides has been commonly observed in both in vivo and in vitro studies, 19 , 20 raising a compelling question about how these receptors receive and integrate information from diverse neuropeptides. The existence of receptor functional diversity challenges our understanding of the complexity and specificity of neuropeptide signaling in neural circuits.…”

Section: Introductionmentioning

confidence: 99%

CKR-1 orchestrates two motor states from a single motoneuron in C. elegans

Chen,

Su,

Wang

et al. 2024

iScience

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“…In this context, C. elegans , which has well-established genetic tools and connectome, has been explored as a model system for the investigation of phoretic behavior at all stages, from the genes involved to the resulting neural circuitry to evolution. For example, the core components of the neuronal circuits and various modulatory pathways involved in nictation have been uncovered ( 9 , 10 , 18 – 22 ). In addition, wild C. elegans strains and their whole-genome sequences, which are available through the Caenorhabditis Natural Diversity Resource (CaeNDR) ( 23 ), provide an opportunity to study the evolution of this behavior.…”

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confidence: 99%

Glial expression of a steroidogenic enzyme underlies natural variation in hitchhiking behavior

Yang,

Lee,

Kim

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Phoresy is an interspecies interaction that facilitates spatial dispersal by attaching to a more mobile species. Hitchhiking species have evolved specific traits for physical contact and successful phoresy, but the regulatory mechanisms involved in such traits and their evolution are largely unexplored. The nematode Caenorhabditis elegans displays a hitchhiking behavior known as nictation during its stress-induced developmental stage. Dauer-specific nictation behavior has an important role in natural C. elegans populations, which experience boom-and-bust population dynamics. In this study, we investigated the nictation behavior of 137 wild C. elegans strains sampled throughout the world. We identified species-wide natural variation in nictation and performed a genome-wide association mapping. We show that the variants in the promoter of nta-1 , encoding a putative steroidogenic enzyme, underlie differences in nictation. This difference is due to the changes in nta-1 expression in glial cells, which implies that glial steroid metabolism regulates phoretic behavior. Population genetic analysis and geographic distribution patterns suggest that balancing selection maintained two nta-1 haplotypes that existed in ancestral C. elegans populations. Our findings contribute to further understanding of the molecular mechanism of species interaction and the maintenance of genetic diversity within natural populations.

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Mass Spectrometry–Driven Discovery of Neuropeptides Mediating Nictation Behavior of Nematodes

Cited by 8 publications

References 89 publications

Optimizing Entomopathogenic Nematode Genetics and Applications for the Integrated Management of Horticultural Pests

Optimizing Entomopathogenic Nematode Genetics and Applications for the Integrated Management of Horticultural Pests

CKR-1 orchestrates two motor states from a single motoneuron in C. elegans

Glial expression of a steroidogenic enzyme underlies natural variation in hitchhiking behavior

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