Mass Spectrometry Imaging of Metabolites in Barley Grain Tissues

Peukert, Manuela; Lim, Wai Li; Seiffert, Udo; Matros, Andrea

doi:10.1002/cppb.20037

Cited by 12 publications

(3 citation statements)

References 35 publications

(65 reference statements)

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“…Sectioning of barley grains and matrix application was performed following Peukert et al (2014). The MS imaging (MSI) measurements were performed using an ultraﬂeXtreme matrix-assisted laser desorption/ionization (MALDI) time-of-ﬂight (TOF)/TOF device (Bruker Daltonics) following Peukert et al (2016a).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of HvCslF6 in barley grain alters carbohydrate partitioning plus transfer tissue and endosperm development

Lim

Collins

Byrt

et al. 2019

Journal of Experimental Botany

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Section: Methodsmentioning

confidence: 99%

Overexpression of HvCslF6 in barley grain alters carbohydrate partitioning plus transfer tissue and endosperm development

Lim

Collins

Byrt

et al. 2019

Journal of Experimental Botany

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“…Hence, it is essential to quantify and identify changes in metabolite composition through proper analytical methods. Nowadays, various integrated technologies are used, such as nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), capillary electrophoresis-MS (CE-MS), liquid chromatography-MS (LC-MS), gas chromatography-MS (GC-MS), and Fourier transform ion cyclotron resonance-MS (FT-ICR-MS) Vinaixa et al 2016;Peukert et al 2016;Mitchell et al 2018). With the advancement of MS-imaging techniques, including matrix-assisted laser desorption (MALDI) and desorption electrospray (DESI) ionization platforms combined with high-resolution MS, it is feasible to conduct in-situ metabolome analysis (Blanksby and Mitchell 2010).…”

Section: Low Molecular Weight Metabolitesmentioning

confidence: 99%

Metabolomics: a systems biology approach for enhancing heat stress tolerance in plants

Raza

2020

Plant Cell Rep

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Key message Comprehensive metabolomic investigations provide a large set of stress-related metabolites and metabolic pathways, advancing crops under heat stress conditions. Metabolomics-assisted breeding, including mQTL and mGWAS boosted our understanding of improving numerous quantitative traits under heat stress. Abstract During the past decade, metabolomics has emerged as a fascinating scientific field that includes documentation, evaluation of metabolites, and chemical methods for cell monitoring programs in numerous plant species. A comprehensive metabolome profiling allowed the investigator to handle the comprehensive data groups of metabolites and the equivalent metabolic pathways in an extraordinary manner. Metabolomics, together with transcriptomics, plays an influential role in discovering connections between stress and genes/metabolite, phenotyping, and biomarkers documentation. Further, it helps to decode several metabolic systems connected with heat stress (HS) tolerance in plants. Heat stress is a critical environmental factor that is globally affecting the growth and productivity of plants. Thus, there is an urgent need to exploit modern breeding and biotechnological tools like metabolomics to develop cultivars with improved HS tolerance. Several studies have reported that amino acids, carbohydrates, nitrogen metabolisms, etc. and metabolites involved in the biosynthesis and catalyzing actions play a game-changing role in HS response and help plants to cope with the HS. The use of metabolomicsassisted breeding (MAB) allows a well-organized transmission of higher yield and HS tolerance at the metabolome level with specific properties. Progressive metabolomics systematic techniques have accelerated metabolic profiling. Nonetheless, continuous developments in bioinformatics, statistical tools, and databases are allowing us to produce ever-progressing, comprehensive insights into the biochemical configuration of plants and by what means this is inclined by genetic and environmental cues. Currently, assimilating metabolomics with post-genomic platforms has allowed a significant division of genetic-phenotypic connotation in several plant species. This review highlights the potential of a state-of-the-art plant metabolomics approach for the improvement of crops under HS. The development of plants with specific properties using integrated omics (metabolomics and transcriptomics) and MAB can provide new directions for future research to enhance HS tolerance in plants to achieve a goal of "zero hunger".

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“…In addition, the type and number of constituent sugars, and the mode of binding between them adds to the diversity of carbohydrates. The distribution of carbohydrates in foods such as strawberries [42,69], barley grain [55], maize seeds [66], and apples [47,82] were analyzed by DESI and MALDI-MSI (Table 3). Horikawa et al confirmed the distribution of hexose, sucrose, and sorbitol in apples by MALDI-MSI; there was a linear correlation between the results of HPLC analysis and the ion intensity obtained by MSI, which demonstrated that MSI can quantify simple sugars in tissue sections [82].…”

Section: Carbohydratesmentioning

confidence: 99%

Application of Mass Spectrometry Imaging for Visualizing Food Components

Yoshimura

Zaima

2020

Foods

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Consuming food is essential for survival, maintaining health, and triggering positive emotions like pleasure. One of the factors that drive us toward such behavior is the presence of various compounds in foods. There are many methods to analyze these molecules in foods; however, it is difficult to analyze the spatial distribution of these compounds using conventional techniques, such as mass spectrometry combined with high-performance liquid chromatography or gas chromatography. Mass spectrometry imaging (MSI) is a two-dimensional ionization technology that enables detection of compounds in tissue sections without extraction, purification, separation, or labeling. There are many methods for ionization of analytes, including secondary ion mass spectrometry, matrix-assisted laser desorption/ionization, and desorption electrospray ionization. Such MSI technologies can provide spatial information on the location of a specific analyte in food. The number of studies utilizing MSI technologies in food science has been increasing in the past decade. This review provides an overview of some of the recent applications of MSI in food science and related fields. In the future, MSI will become one of the most promising technologies for visualizing the distribution of food components and for identifying food-related factors by their molecular weights to improve quality, quality assurance, food safety, nutritional analysis, and to locate administered food factors.

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Mass Spectrometry Imaging of Metabolites in Barley Grain Tissues

Cited by 12 publications

References 35 publications

Overexpression of HvCslF6 in barley grain alters carbohydrate partitioning plus transfer tissue and endosperm development

Overexpression of HvCslF6 in barley grain alters carbohydrate partitioning plus transfer tissue and endosperm development

Metabolomics: a systems biology approach for enhancing heat stress tolerance in plants

Application of Mass Spectrometry Imaging for Visualizing Food Components

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