Mass Spectrometry of Carbohydrates

Structural studies of the lipopolysaccharide from Haemophilus parainfluenzae strain 20

“…In addition, the oligosaccharides can be decorated by non-carbohydrate structures. Mass spectrometry is a very powerful and useful tool for the structural analysis of lipopolysaccharides (87).…”

Tandem mass spectra of ammonium adducts of monosaccharides: differentiation of diastereomers

“…Much research has been undertaken focused on the determination of carbohydrate structures using mass spectrometry, initially employing gas phase ionization or desorption/ionization techniques 6–8. Fast atom bombardment has also found wide applications in the structure analysis of carbohydrates 9, 10. The advent of electrospray ionization (ESI)11 and matrix‐assisted laser desorption ionization (MALDI)12 techniques has considerably facilitated the analysis of polar molecules such as carbohydrates.…”

Exopolysaccharides from Prokaryotic Microorganisms—Promising Sources for White Biotechnology Processes

“…The chromatogram showed the presence of 1,3,4,6-tetra-O-methyl(2,5-di-O-acetyl)-mannitol and -glucitol, 1,3,4-tri-O-methyl(2,5,6-tri-O-acetyl)-mannitol and -glucitol, and 3,4-di-O-methyl(1,2,5,6-tetra-O-acetyl)-mannitol and -glucitol, which corresponded to t-Fruf, 6-Fruf, and 1,6-Fruf, with the following % mole of partially methylated alditol acetates as 1%, 10%, and 1%, respectively. The reduction at C-2 of fructose was performed by using NaBD 4 , allowing to discriminate between 6-Fruf and 1-Fruf, 9 suggesting that this polymer was composed by beta-(2,6)-d-fructofuranosyl residues.…”