Massilia psychrophila sp. nov., isolated from an ice core

Guo, Bixi; Liu, Yongqin; Gu, Zhengquan; Shen, Liang; Liu, Keshao; Wang, Ningliang; Xing, Tingting; Liu, Hong‐Can; Zhou, Yu‐Guang; Li, Jiule

doi:10.1099/ijsem.0.001315

Cited by 28 publications

(17 citation statements)

References 30 publications

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“…These species included Massilia sp. also identified from the Muztagh Glacier, China (Shen et al, 2015; Guo et al, 2016), and Janthinobacterium sp. reported from Qinghai-Tibet Plateau permafrost (Zhang et al, 2007) and tundra soil [ J. lividum (MH929893)].…”

Section: Discussionmentioning

confidence: 99%

Total and Potentially Active Bacterial Communities Entrapped in a Late Glacial Through Holocene Ice Core From Scarisoara Ice Cave, Romania

Paun¹,

Icaza

Lavín

et al. 2019

Front. Microbiol.

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Our understanding of the icy-habitat microbiome is likely limited by a lack of reliable data on microorganisms inhabiting underground ice that has accumulated inside caves. To characterize how environmental variation impacts cave ice microbial community structure, we determined the composition of total and potentially active bacterial communities along a 13,000-year-old ice core from Scarisoara cave (Romania) through 16S rRNA gene Illumina sequencing. An average of 2,546 prokaryotic gDNA operational taxonomic units (OTUs) and 585 cDNA OTUs were identified across the perennial cave ice block and analyzed in relation to the geochemical composition of ice layers. The total microbial community and the putative active fraction displayed dissimilar taxa profiles. The ice-contained microbiome was dominated by Actinobacteria with a variable representation of Proteobacteria, while the putative active microbial community was equally shared between Proteobacteria and Firmicutes. Accordingly, a major presence of Cryobacterium, Lysinomonas, Pedobacter , and Aeromicrobium phylotypes homologous to psychrotrophic and psychrophilic bacteria from various cold environments were noted in the total community, while the prevalent putative active bacteria belonged to Clostridium, Pseudomonas, Janthinobacterium, Stenotrophomonas , and Massilia genera. Variation in the microbial cell density of ice strata with the dissolved organic carbon (DOC) content and the strong correlation of DOC and silicon concentrations revealed a major impact of depositional processes on microbial abundance throughout the ice block. Post-depositional processes appeared to occur mostly during the 4,000–7,000 years BP interval. A major bacterial composition shift was observed in 4,500–5,000-year-old ice, leading to a high representation of Beta- and Deltaproteobacteria in the potentially active community in response to the increased concentrations of DOC and major chemical elements. Estimated metabolic rates suggested the presence of a viable microbial community within the cave ice block, characterized by a maintenance metabolism in most strata and growth capacity in those ice deposits with high microbial abundance and DOC content. This first survey of microbial distribution in perennial cave ice formed since the Last Glacial period revealed a complex potentially active community, highlighting major shifts in community composition associated with geochemical changes that took place during climatic events that occurred about 5,000 years ago, with putative formation of photosynthetic biofilms.

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Section: Discussionmentioning

confidence: 99%

Total and Potentially Active Bacterial Communities Entrapped in a Late Glacial Through Holocene Ice Core From Scarisoara Ice Cave, Romania

Paun¹,

Icaza

Lavín

et al. 2019

Front. Microbiol.

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“…The first Massilia species was isolated from the blood of an immunocompromised patient in 1998 [7]. Massilia is a niche divergent family of bacteria and, since their first discovery, species of the genus Massilia have been isolated from marama bean and poplar tissue samples [12,13], water [14,15], air [16] and ice cores [17]. Massilia organisms are most commonly isolated from soil samples and have been found to colonize root surfaces and are relatively abundant in the rhizosphere [18,19].…”

Section: Introductionmentioning

confidence: 99%

Massilia arenosa sp. nov., isolated from the soil of a cultivated maize field

Raths

Peta

Bücking

2020

International Journal of Systematic and Evolutionary Microbiology

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Strain MC02T, a Gram-stain-negative, rod-shaped bacterium, was isolated from field soil collected from California, USA. To examine if MC02T represents a novel species, we compared its colony morphology, 16S rRNA gene and whole genome sequence, and its metabolic phenotype using Biolog GenIII and MALDI-TOF analyses compared to reference strains. Based on 16S rRNA gene and whole genome sequencing, MC02T belongs to the genus Massilia and Massilia agri K-3–1T is the most similar strain with 96.97 % 16S rRNA gene sequence identity. MALDI-TOF analysis revealed that Massilia aerilata DSM19289T is the closest match, but the similarity score was much lower than the ≥1.7 threshold for a reliable identification at the genus level. The predominant fatty acids were summed feature 3 (C16 : 1⍵7c and/or C16 : 1⍵6c; 49.07 %) and C16 : 0 (30.01 %). The genome is 5.02 Mbp and the G+C content is 66.2 mol%. Whole genome comparisons to the closest related strains revealed an average amino acid identity value of 67.4 %, an OrthoANI similarity of 77.1 %, and a DNA–DNA-hybridization probability ≥70 %, confirming that MC02T represents a novel species. Strain MC02T can grow at pH 6 but not at pH 5, and a salt concentration of ≥1 % inhibits its growth. In contrast to other Massilia strains, MC02T can utilize turanose, inosine and l-serine. The genome of MC02T shows putative endophyte genes such as a nitrate reductase, several phosphatases, and biotin biosynthesis genes, 26 flagellar motility genes and 14 invasion and intracellular resistance genes. Based on its metabolic, physiological and genomic characteristics, we propose that strain MC02T (NRRL B-65554T=ATCC TSD-200T=LMG 31737T) represents a novel species of the genus Massilia with the name Massilia arenosa sp. nov.

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“…The first species within the genus Massilia was isolated from clinical samples (1). Since then, Massilia species have been isolated from plant tissues (2), water (3), air (4), ice cores (5), and soils (6). Massilia spp.…”

Section: Announcementmentioning

confidence: 99%