Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus

Fonseca, Tiago Silva da; Silva, G. F.; Guerreiro-Tanomaru, Juliane Maria; Sasso‐Cerri, Estela; Tanomaru‐Filho, Mário; Cerri, Paulo Sérgio

doi:10.1111/iej.12981

Cited by 18 publications

(39 citation statements)

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“…The CE-1 sealer had a reduced inflammatory infiltrated when compared to AH Plus, showing a connective tissue with scarce inflammatory cells dispersed amongst collagen fibres and fibroblasts. The presence of fibroblasts in the 60-day period indicates progressive tissue repair (Silva et al 2015, da Fonseca et al 2019. Zordan-Bronzel et al (2019) demonstrated that the experimental sealer used in this study was cytocompatible with potential for use as a root canal filling material.…”

Section: --mentioning

confidence: 77%

Evaluation of the biological properties of two experimental calcium silicate sealers: an in vivo study in rats

et al. 2020

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Aim To evaluate the biological properties of experimental sealers based on tricalcium silicate and dicalcium silicate, manipulated with polyethylene glycol (CE‐1) and with the addition of calcium hypochlorite (CE‐2) compared to AH Plus (AHP) and TotalFill BC Sealer (TBC). Methodology The tissue reaction caused by the materials in the subcutaneous tissue of rats was evaluated after implantation of polyethylene tubes filled with the materials at 7, 15, 30 and 60 days. Sections were stained with haematoxylin and eosin (HE) for morphological analysis and to evaluated number of inflammatory cells/mm2 (ICs). Sections were used for immunohistochemical detection of interleukin‐6 (IL‐6) and osteocalcin (OC). The von Kossa method was used to identify calcium precipitation in the capsules. The data were submitted to anova and Tukey’s tests, with 5% significance level. Results At 7 days, CE‐1, CE‐2 and AHP had higher numbers of ICs. AHP presented higher immunolabelling for IL‐6. After 15 days, regarding IL‐6, there was no difference between CE‐2 and the control group. At 30 days, AHP exhibited the highest number of IC (P < 0.05) and CE‐2 and the control group presented the lowest ICs and IL‐6‐positive cells. After 60 days, all materials exhibited decreases in ICs. CE‐2, TBC and the control had the lowest values (P < 0.05). No significant difference was detected between CE‐1 and TBC, and between CE‐2 and control considering the immunoexpression of IL‐6. In this period, AHP had the greatest number of IC and IL‐6 (P < 0.05). In all periods, CE‐1, CE‐2 and TBC sealers had von Kossa‐positive structures and OC‐immunolabelled cells. CE‐2 had higher number of OC‐positive cells than the CE‐1 and TBC sealers (P < 0.05), in all periods. OC immunolabelling was not observed in the capsules of AH Plus and the control groups. Conclusions The experimental sealer and its association with calcium hypochlorite, in addition to TotalFill BC Sealer, were biocompatible and had bioactive potential.

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Section: --mentioning

confidence: 77%

Evaluation of the biological properties of two experimental calcium silicate sealers: an in vivo study in rats

et al. 2020

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“…Previous reports have focused on whether Biodentine or MTA may cause inflammation (da Fonseca et al . 2016, 2019) or how normal tissues react to the presence of either Biodentine or MTA (Miller et al . 2018, Giraud et al .…”

Section: Discussionmentioning

confidence: 99%

“…Biodentine (Septodont Inc., Saint‐Maur‐des‐Fossés, France) is used during pulp capping and REPs. Biodentine is not only biocompatible (da Fonseca et al . 2019) but also bioinductive when applied to dental tissues (Miller et al . 2018).…”

Section: Introductionmentioning

confidence: 99%

The molecular functions of Biodentine and mineral trioxide aggregate in lipopolysaccharide‐induced inflamed dental pulp cells

Wang

Chang

et al. 2021

Int Endodontic J

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Aim To explore the proliferation, adhesion and differentiation response and the underlying mechanisms that occur in lipopolysaccharide (LPS)-induced inflamed dental pulp cells (DPCs) in contact with Biodentine and mineral trioxide aggregate (MTA). Methodology The DPCs were isolated from three healthy donors and named DPC-H1 to DPC-H3. The DPCs were pre-cultured with 2 or 5 lg mL À1 LPS for 24 h to induce inflammation. The expression of inflammation marker miR-146a was detected by q-PCR. The normal and LPS-induced DPCs were further treated with 0.14 mg mL À1 Biodentine or 0.13 mg mL À1 MTA for 24 h. MTT assay and adhesion assay were used to analyse the changes of cell phenotypes. DSPP, AKT and ERK expressions were detected by Western blotting. The data were analysed by Mann-Whitney test or two-way ANOVA. Differences were considered statistically significant when P < 0.05. Results In LPS-induced DPCs, Biodentine and MTA treatment neither induced nor aggravated LPS-

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“…The thicknesses of apical plugs and different irrigation agents are reported to affect the apical leakage. Twelve millimeters of Bioaggregate exhibits the best resistance to leakage while Ethylenediaminetetraacetic acid (EDTA) and a Shahi et al, 2010;Cintra et al, 2013;Bueno et al, 2019 • Thick and loose fibrous capsule formation on day 7 and was replaced by thin and dense fibrous capsule formation finally Cintra et al, 2013;Taha et al, 2016;Bueno et al, 2019 • The thickness of fibrous capsules increased over time Khalil and Abunasef, 2015 • Dystrophic calcification and birefringent structure Viola et al, 2012;Cintra et al, 2013;Hinata et al, 2017;Bueno et al, 2019 • Enhanced M2 macrophage polarization Ito et al, 2014 Mouse • Induced the acute inflammation and biomineralization simultaneously Reyes-Carmona et al, 2010 Bioaggregate Rat • Less inflammatory response and produce less calcification compared to MTA Batur et al, 2013 • More inflammatory response compared to MTA Saghiri et al, 2013b • Similar biocompatibility to MTA Bosio et al, 2014 • Adverse effect on liver function and kidney function Khalil and Eid, 2013;Simsek et al, 2016 Biodentine Rat • Intense inflammation on day 7 and decreased over time Mori et al, 2014;Pinheiro et al, 2018 • Fibrous capsules formation Da et al, 2016Da et al, , 2019…”

Section: Bioaggregatementioning

confidence: 99%

In vivo Biocompatibility and Bioactivity of Calcium Silicate-Based Bioceramics in Endodontics

Song

Sun

Chen

et al. 2020

Front. Bioeng. Biotechnol.

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Endodontic therapy aims to preserve or repair the activity and function of pulp and periapical tissues. Due to their excellent biological features, a substantial number of calcium silicate-based bioceramics have been introduced into endodontics and simultaneously increased the success rate of endodontic treatment. The present manuscript describes the in vivo biocompatibility and bioactivity of four types of calcium silicate-based bioceramics in endodontics.

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Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus

Abstract: The collagen-rich capsule formed slowly around Biodentine in comparison with MTA. FGF-1 and mast cells participated in capsule remodelling, stimulating fibroblast proliferation and subsequent collagen production, in response to subcutaneous implants.

Cited by 18 publications

References 50 publications

Evaluation of the biological properties of two experimental calcium silicate sealers: an in vivo study in rats

Evaluation of the biological properties of two experimental calcium silicate sealers: an in vivo study in rats

The molecular functions of Biodentine and mineral trioxide aggregate in lipopolysaccharide‐induced inflamed dental pulp cells

In vivo Biocompatibility and Bioactivity of Calcium Silicate-Based Bioceramics in Endodontics

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