Mast cells contribute to altered vascular reactivity and ischemia-reperfusion injury following cerium oxide nanoparticle instillation

Wingard, Christopher J.; Walters, Dianne M.; Cathey, Brook L.; Hilderbrand, Susana C.; Katwa, Pranita; Lin, Sijie; Ke, Pu Chun; Podila, Ramakrishna; Rao, Apparao M.; Lust, Robert M.; Brown, Jared M.

doi:10.3109/17435390.2010.530004

Cited by 80 publications

(74 citation statements)

References 42 publications

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“…Moreover, this time point is identical to the one used recently to assess the role of mast cells in vascular reactivity and ischemia reperfusion injury following IT administration of CeO 2 NPs. 34 These data show that acute administration of CeO 2 NPs caused a dose-dependent infiltration of neutrophils and macrophages, which caused an expansion of the alveolar interstitial space. Some CeO 2 NPs were engulfed by macrophages, and others gained access to the interstitium.…”

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confidence: 69%

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The acute pulmonary and thrombotic effects of cerium oxide nanoparticles after intratracheal instillation in mice

Al-Salam¹,

Beegam²,

Yuvaraju³

et al. 2017

IJN

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Cerium oxide nanoparticles (CeO 2 NPs), used as a diesel fuel catalyst, can be emitted into the ambient air, resulting in exposure to humans by inhalation. Recent studies have reported the development of lung toxicity after pulmonary exposure to CeO 2 NPs. However, little is known about the possible thrombotic effects of these NPs. The present study investigated the acute (24 hours) effect of intratracheal (IT) instillation of either CeO 2 NPs (0.1 or 0.5 mg/kg) or saline (control) on pulmonary and systemic inflammation and oxidative stress and thrombosis in mice. CeO 2 NPs induced a significant increase of neutrophils into the bronchoalveolar lavage (BAL) fluid with an elevation of tumor necrosis factor α (TNFα) and a decrease in the activity of the antioxidant catalase. Lung sections of mice exposed to CeO 2 NPs showed a dose-dependent infiltration of inflammatory cells consisting of macrophages and neutrophils. Similarly, the plasma levels of C-reactive protein and TNFα were significantly increased, whereas the activities of catalase and total antioxidant were significantly decreased. Interestingly, CeO 2 NPs significantly and dose dependently induced a shortening of the thrombotic occlusion time in pial arterioles and venules. Moreover, the plasma concentrations of fibrinogen and plasminogen activator inhibitor-1 were significantly elevated by CeO 2 NPs. The direct addition of CeO 2 NPs (1, 5, or 25 μg/mL) to mouse whole blood, collected from the inferior vena cava, in vitro neither caused significant platelet aggregation nor affected prothrombin time or partial thromboplastin time, suggesting that the thrombotic events observed in vivo may have resulted from systemic inflammation and/or oxidative stress induced by CeO 2 NPs. This study concludes that acute pulmonary exposure to CeO 2 NPs induces pulmonary and systemic inflammation and oxidative stress and promotes thrombosis in vivo.

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confidence: 69%

“…32,33 The doses of particles used in the present study are comparable with those employed in previous animal models of IT administration of CeO 2 NPs. 12,34 In a previous study, it has been demonstrated that acute (24 hours) exposure of mice to DEPs causes impairment …”

Section: Discussionmentioning

confidence: 99%

The acute pulmonary and thrombotic effects of cerium oxide nanoparticles after intratracheal instillation in mice

Al-Salam¹,

Beegam²,

Yuvaraju³

et al. 2017

IJN

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“…Particularly, studies related to in vivo interaction of NPs with mast cells are limited to clinical reports only 6 . Mast cells are derived from hematopoeitic precursor cells in the bone marrow and once they attain maturity migrate to the connective tissues of the body 7 .…”

Section: Introductionmentioning

confidence: 99%

“…DEX is known to elicit IgG and IgE antibody-mediated reactions and this could be correlated to the increased mast cells in DEX-SPIONS group in lungs. It has been shown that inhalation of cerium oxide nanoparticles can activate mast cells by promoting the release of TH 2 proinflammatory cytokines and resulting in altered vascular reactivity6 . Furthermore, carbon nanotube induced toxicities are mediated by mast cell activation in both the pulmonary and cardiovascular system55 .…”

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confidence: 99%

Effect of surface-modified superparamagnetic iron oxide nanoparticles (SPIONS) on mast cell infiltration: An acute in vivo study

Sabareeswaran

Ansar

Varma

et al. 2016

Nanomedicine: Nanotechnology, Biology and Medicine

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“…12 , 16 -18 As an example, we discuss the use of Raman spectroscopy in elucidating the biodistribution and immune response of CeO 2 NPs. 18 The risk of CeO 2 NP exposure is signifi cant since they are widely used in many applications, including solar cells and as fuel additives. Instillation of CeO 2 NPs in the lungs of mice was shown to activate mast cells, which play an important role in allergic immune responses, tissue repair, and the maintenance of homeostasis.…”

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confidence: 99%

Illuminating nano-bio interactions: A spectroscopic perspective

et al. 2014

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IntroductionEngineered nanomaterials (ENMs) provide much-needed breakthroughs for resolving some longstanding challenges in drug delivery and personalized medicine. They exhibit pronounced interactions with biomolecules and the ability to effi ciently cross many physiological barriers due to their small size, which may also cause undesired side effects (also see the Wick et al., Hemmer et al., and Bhattacharya et al. articles in this issue). 1 -5 Spectroscopic tools can be used to determine the underlying biophysical mechanisms and the nature of nanobio interactions at the cellular, subcellular, and protein levels, which cannot be gleaned from traditional toxicological studies. For example, some ENMs release metal ions through dissolution at varying rates in different media, including blood. 6 -11 As discussed in this article, these metal ions can adversely affect the proper functioning of biomolecules and cells through an "action-at-a-distance" mechanism. Spectroscopic tools can clearly identify the net bioavailable metal ion content and help establish relationships between ion-release from ENM and ENM-induced toxicity. As outlined in Figure 1 , this article discusses how various spectroscopic tools (e.g., Raman, photoluminescence, infrared, and surface plasmon) can probe the intrinsic properties of ENMs (e.g., crystallinity and electronic states) and biomolecules (e.g., protein structure) and provide a comprehensive understanding of ENM transport within organisms (e.g., via biodistribution) and new perspectives of biological interactions (e.g., loss of protein structure upon adsorption on ENM) at the nanoscale. Fluorescence imaging of ENM biodistributionThe ultimate prerequisite for a harmful effect from any toxicant is exposure. Therefore, tools allowing the visualization of the nanoparticles (NPs) in vivo are crucial for risk evaluation. On the other hand, the growing utilization of ENMs in potential clinical applications ranging from diagnosis to therapeutic treatment of diseases necessitates a need for understanding their in vivo transport, biodistribution, and long-term fate in the organism. 12 ENMs are known to exhibit a higher uptake by monocytes and macrophages of the mononuclear phagocyte system (a part of the immune system containing phagocytic cells) upon injection into animals, which prevents their rapid clearance through excretion and impairs therapeutic or diagnostic targeting. 2 , 12 Indeed, the clearance mechanisms for most ENMs remain unresolved due to the existing diffi culties in in vivo tracking and monitoring of the ENMs. Fluorescence spectroscopy is one of the important tools in assessing the biological interaction Engineered nanomaterials (ENMs) strongly interact with biomolecules and cells due to their similar size scales. Consequently, ENMs are beginning to emerge as new medical diagnostic tools, probes in cell biology, and delivery vehicles, compelling us to understand the interactions at the nano-bio interface. Optical spectroscopic tools are excellent probes to characterize ENMs...

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Mast cells contribute to altered vascular reactivity and ischemia-reperfusion injury following cerium oxide nanoparticle instillation

Cited by 80 publications

References 42 publications

The acute pulmonary and thrombotic effects of cerium oxide nanoparticles after intratracheal instillation in mice

The acute pulmonary and thrombotic effects of cerium oxide nanoparticles after intratracheal instillation in mice

Effect of surface-modified superparamagnetic iron oxide nanoparticles (SPIONS) on mast cell infiltration: An acute in vivo study

Illuminating nano-bio interactions: A spectroscopic perspective

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