MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels

Närvä, Elisa; Taskinen, Maria Emilia; Lilla, Sérgio; Isomursu, Aleksi; Pietilä, Mika; Weltner, Jere; Isola, Jorma; Sihto, Harri; Joensuu, Heikki; Zanivan, Sara; Norman, Jim; Ivaska, Johanna

doi:10.1016/j.isci.2022.104459

Cited by 3 publications

(2 citation statements)

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“…In addition, in the present results, early and repeated experiences of painful stimulation caused a signi cant upregulation of MASTL in adolescent hippocampal tissue and were involved in regulating chromosome condensation and cell division processes (19)(20)(21). MASTL is active in cell cycle progression and induces the mitotic transition by inhibiting the activity of the tumor suppressor PP2A-B55 phosphatase (22).…”

Section: Discussionsupporting

confidence: 61%

Proteomics of long-term neurodevelopmental disorders of rats exposed to early pain stimulation in the neonatal period

Zuo

et al. 2023

Preprint

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Objective To screen the feasibility and value of quantitative proteomic tandem mass spectrometry labeling (TMT) technology for early pain stimulation leading to neurodevelopmental disorders during puberty in neonatal rats. Methods The rats were randomly divided into two groups at postnatal day one (P1), and the skin was pricked for seven consecutive days in the experimental group; the control group was stroked simultaneously. A Morris water maze experiment was performed at P45 and P48. The rats were sacrificed at P50, and the proteins extracted from the hippocampal tissues were analyzed by TMT quantitative proteomics. The differentially expressed proteins were identified as biologically relevant proteins after pain intervention. Results The Morris water maze experiment suggested that the experimental group of rats had a significantly longer escape latency at P45 and P48 than the control group (P<0.05). At P49, Rats in the experimental group crossed the traversing platform less often than the control group within 60 s. Hippocampal tissue proteomics analysis showed than 33 proteins were upregulated, and 37 were downregulated. The differentially expressed proteins were enriched in different GO subsets, the most significant of which were sucrose metabolic process, response to stilbenoid maltose metabolic process, disaccharide metabolic process, circulating immunoglobulin complex, haptoglobin-hemoglobin complex, hemoglobin complex, replisome, purine-rich negative regulatory element binding, biliverdin reductase activity, alpha-1,4-glucosidase activity, and retinal dehydrogenase activity. KEGG enrichment analysis was most significant for starch and sucrose metabolism, galactose metabolism, antigen processing and presentation, and TGF-beta signaling. The core proteins were identified by constructing protein interaction networks. Four essential differentially expressed proteins were screened, including microtubule-associated serine-/threonine-protein kinase, carbonic anhydrase 3, F-box and leucine-rich repeat protein 4, and NADH-ubiquinone oxidoreductase chain 4L. Conclusions TMT-labeled proteomics identified energy metabolic processes, developmental processes, cellular processes, bioregulation, and signaling as the main targets of distant neurodevelopmental disorders caused by painful stimulation. Microtubule-associated serine/threonine-protein kinase, carbonic anhydrase 3, F-box and leucine-rich repeat protein 4, and NADH-ubiquinone oxidoreductase chain 4L may be involved in the cognitive impairment of adolescence caused by early pain stimulation in neonatal rats.

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Section: Discussionsupporting

confidence: 61%

Proteomics of long-term neurodevelopmental disorders of rats exposed to early pain stimulation in the neonatal period

Zuo

et al. 2023

Preprint

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“…Nanog induces an undifferentiated state by repressing Gata4 and upregulating Rex1 and LIF-responsive genes, in particular Esrrb [52][53][54][55]. Oct4 is another central factor that can sustain intrinsic signaling in a LIF-independent manner to promote ES cell pluripotency and self-renewal [56,57]. Natriuretic peptide receptor A (NPR-A) upregulates gene expression of pluripotency markers (Nanog, Oct4, and Sox2) and increases levels of phosphorylated Akt, hence promoting self-renewal and pluripotency of ESCs [58].…”

Section: Murine Escs: Signaling Pathways Supporting Self-renewal and ...mentioning

confidence: 99%

Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells

Varzideh

Gambardella

Kansakar

et al. 2023

IJMS

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Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the blastocyst. ESCs have two distinctive properties: ability to proliferate indefinitely, a feature referred as “self-renewal”, and to differentiate into different cell types, a peculiar characteristic known as “pluripotency”. Self-renewal and pluripotency of ESCs are finely orchestrated by precise external and internal networks including epigenetic modifications, transcription factors, signaling pathways, and histone modifications. In this systematic review, we examine the main molecular mechanisms that sustain self-renewal and pluripotency in both murine and human ESCs. Moreover, we discuss the latest literature on human naïve pluripotency.

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Targeted inhibition of MASTL kinase activity induces apoptosis in breast cancer

Misra,

Rajawat,

Pal

et al. 2023

Life Sciences

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MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels

Cited by 3 publications

References 70 publications

Proteomics of long-term neurodevelopmental disorders of rats exposed to early pain stimulation in the neonatal period

Proteomics of long-term neurodevelopmental disorders of rats exposed to early pain stimulation in the neonatal period

Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells

Targeted inhibition of MASTL kinase activity induces apoptosis in breast cancer

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