Mastoid Biofilm in Chronic Otitis Media

Lampikoski, Henri; Aarnisalo, Antti A.; Jero, Jussi; Kinnari, Teemu J.

doi:10.1097/mao.0b013e318259533f

Cited by 39 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was lower compared to prior studies in which it ranged from 60-70 per cent [6,21,22]. Staphylococcus aureus was the predominant biofilm former with 47.36 per cent of the isolates testing positive for biofilm formation.…”

Section: Discussioncontrasting

confidence: 43%

Spectrum and Antibiogram of Bacteria in Chronic Suppurative Otitis Media and Biofilm Formation

Banu

Samhitha

Presteena

et al. 2017

JSRT

View full text Add to dashboard Cite

Context: Chronic suppurative otitis media (CSOM) is a common infection having a high prevalence rate of 7.8-16% in India and is one of the leading causes of conductive deafness particularly in the adolescent age group [1]. CSOM is frequently associated with multidrug-resistant organisms with the ability to form biofilm, which is an important virulence factor and results in treatment failure. Aims:The main objectives of the study are to identify the spectrum of bacteria associated with CSOM, their antibiotic sensitivity pattern, and to detect the biofilm formation.Methods: This was a prospective cross-sectional study at a tertiary care hospital. Patients upto the age of 80, having otorrhea for more than 6-12 weeks [2,3] and attending the ENT outpatient department were included. Samples of pus were collected from the deeper aspect of external auditory meatus and processed using standard techniques for culture and sensitivity. Biofilm detection was done. Results were compiled and statistically analysed.Results: Sixty samples were processed and 43 yielded positive cultures. Staphylococcus aureus was the predominant organism, followed by Pseudomonas aeruginosa. Biofilm formation was seen in 19 (44.84 per cent) of the organisms. Biofilms were formed predominantly by Staphylococcus aureus (47.36 per cent). Conclusion:Multidrug resistance has become increasingly common amongst the causative organisms of CSOM, this trend being particularly evident among biofilm producers. Therefore, screening for biofilm formation, along with the usual antibiogram, needs to be performed as a routine procedure in CSOM to effectively manage the situation by choosing an appropriate modality of treatment.

show abstract

Section: Discussioncontrasting

confidence: 43%

Spectrum and Antibiogram of Bacteria in Chronic Suppurative Otitis Media and Biofilm Formation

Banu

Samhitha

Presteena

et al. 2017

JSRT

View full text Add to dashboard Cite

show abstract

“…In addition, biofilms attach firmly to damaged tissue, such as exposed osteitic bone and ulcerated middle ear mucosa, or to otological implants such as tympanostomy tubes, further aggravating the problem of eradication (Wang et al, 2014). Although biofilms have been demonstrated in the middle ear of CSOM patients, their precise role in the pathophysiology of the disease is yet to be determined (Saunders et al, 2011;Lampikoski et al, 2012;Kaya et al, 2013;Gu et al, 2014;Khosravi et al, 2014). Furthermore, the molecular mechanisms leading to biofilm formation in the middle ear during CSOM are also poorly understood.…”

Section: Pathogenesismentioning

confidence: 99%

Current concepts in the pathogenesis and treatment of chronic suppurative otitis media

Mittal

Lisi

Gerring

et al. 2015

Journal of Medical Microbiology

200

161

View full text Add to dashboard Cite

Otitis media (OM) is an inflammation of the middle ear associated with infection. Despite appropriate therapy, acute OM (AOM) can progress to chronic suppurative OM (CSOM) associated with ear drum perforation and purulent discharge. The effusion prevents the middle ear ossicles from properly relaying sound vibrations from the ear drum to the oval window of the inner ear, causing conductive hearing loss. In addition, the inflammatory mediators generated during CSOM can penetrate into the inner ear through the round window. This can cause the loss of hair cells in the cochlea, leading to sensorineural hearing loss. Pseudomonas aeruginosa and Staphylococcus aureus are the most predominant pathogens that cause CSOM. Although the pathogenesis of AOM is well studied, very limited research is available in relation to CSOM. With the emergence of antibiotic resistance as well as the ototoxicity of antibiotics and the potential risks of surgery, there is an urgent need to develop effective therapeutic strategies against CSOM. This warrants understanding the role of host immunity in CSOM and how the bacteria evade these potent immune responses. Understanding the molecular mechanisms leading to CSOM will help in designing novel treatment modalities against the disease and hence preventing the hearing loss. IntroductionOtitis media (OM) refers to a group of complex infectious and inflammatory diseases affecting the middle ear (Dickson, 2014). OM in general is very common, as studies show that around 80 % of children should have experienced at least one episode by their third birthday (Teele et al., 1989). OM has been broadly classified into two main types, acute and chronic. Acute OM (AOM) is characterized by the rapid onset of signs of inflammation, specifically bulging and possible perforation of the tympanic membrane, fullness and erythema, as well as symptoms associated with inflammation such as otalgia, irritability and fever (Pukander, 1983;Harkness & Topham, 1998). Despite appropriate antibiotic therapy, AOM may progress to chronic suppurative OM (CSOM) characterized by persistent drainage from the middle ear associated with a perforated ear drum (Wintermeyer & Nahata, 1994;Harkness & Topham, 1998). When examined by otoscope, the middle ear looks red and inflamed with purulent discharge in CSOM patients (Figs 1 and 2). It is one of the most common chronic infectious diseases worldwide especially affecting children (Roland, 2002; Verhoeff et al., 2006). Hearing impairment is one of the most common sequelae of CSOM (Aarhus et al., 2015). The resultant hearing loss can have a negative impact on a child's speech development, education and behaviour (Olatoke et al., 2008; Khairi Md Daud et al., 2010). Mortality due to complications of CSOM is typically higher than other types of OM (Yorgancilar et al., 2013a;Qureishi et al., 2014). Intracranial complications like brain abscess and meningitis are the most common causes of death in CSOM patients (Dubey et al., 2010;Chew et al., 2012;Sun & Sun, 2014).In this article, the rec...

show abstract

“…The study also showed higher incidence of bacterial biofilm on CSOM with cholesteatoma compared CSOM without cholesteatoma. The difference between the two CSOM groups in the presence of biofilm was statistically significant (Saunders et al 2011, Lampikoski et al 2012.…”

Section: Biofilm Bacteria Correlation With Cholesteatoma In Csommentioning

confidence: 95%

“…Studies on middle ear tissues in CSOM with cholesteatoma also found biofilms bacteria (Kumar & Prasad 2006, Saunders et al 2011, Lampikoski et al 2012. Until now, direct detection of bacterial biofilms in CSOM by Scanning Electron Microscopy (SEM) has never been done in Surabaya and Indonesia.…”

Section: Introductionmentioning

confidence: 99%

“…A study by Lampikoski et al (2012) obtained bacterial biofilm in 14 (82%) of 17 CSOM patients with cholesteatoma and 5 (42%) tissues had positive bacterial biofilm from 12 tissues of CSOM patients without cholesteatoma, while the control group gained 1 (9%) of 11 patients with cochlear implants. A study by Kaya et al (2013) showed 7 out of 10 CSOM patients had biofilm (70%), 6 of the 11 groups of nonsuppurative chronic otitis media showed biofilm (54.5%).…”

Section: Biofilm Bacteria Correlation With Cholesteatoma In Csommentioning

confidence: 99%

See 1 more Smart Citation

Biofilm Bacteria Plays a Role in Csom Pathogenesis and Has Significant Correlation With Unsafe Type Csom

Artono¹,

Purnami²,

Rahmawati³

2016

FMI

View full text Add to dashboard Cite

ABSTRAKOtitis media supuratif kronik (OMSK) dapat menyebabkan morbiditas dan mortalitas sehingga sampai sekarang masih menjadi masalah kesehatan utama di seluruh dunia. Angka kejadian OMSK di Indonesia (1994-1996 Indonesia (1994Indonesia ( -1996 ABSTRACT Chronic suppurative otitis media (CSOM) may cause severe morbidity and mortality and remains a major health problem worldwide. The incidence of CSOM in

show abstract

Mastoid Biofilm in Chronic Otitis Media

Abstract: Mastoid mucosal biofilm could be seen in patients with COM with or without cholesteatoma. The role of mastoid biofilm in the development of cholesteatoma should be studied further.

Cited by 39 publications

References 23 publications

Spectrum and Antibiogram of Bacteria in Chronic Suppurative Otitis Media and Biofilm Formation

Spectrum and Antibiogram of Bacteria in Chronic Suppurative Otitis Media and Biofilm Formation

Current concepts in the pathogenesis and treatment of chronic suppurative otitis media

Biofilm Bacteria Plays a Role in Csom Pathogenesis and Has Significant Correlation With Unsafe Type Csom

Contact Info

Product

Resources

About