Material properties of disulfide-crosslinked hyaluronic acid hydrogels influence prostate cancer cell growth and metabolism

Tam, Nicky W.; Chung, Dudley; Baldwin, Samuel J.; Simmons, Jeffrey R.; Xu, Liang; Rainey, Jan K.; Dellaire, Graham; Frampton, John P.

doi:10.1039/d0tb01570a

Cited by 10 publications

(6 citation statements)

References 72 publications

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“…The inclusion of HA significantly promoted PCa cellular proliferation, which corresponded with the results found in another study, where the disulfidecrosslinked HA hydrogels could promote PC-3 and LNCaP cell growth [49]. RNA-seq was further used to analyze the effect of HA on PCa cellular behavior more comprehensively.…”

Section: Ha Induced Caf Transformation Through Pca Cell Cytokine Secr...supporting

confidence: 82%

3D bioprinting of multi-cellular tumor microenvironment for prostate cancer metastasis

Huang

et al. 2023

Biofabrication

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Prostate cancer (PCa) is one of the most lethal cancers in men worldwide. The tumor microenvironment (TME) plays an important role in PCa development, which consists of tumor cells, fibroblasts, endothelial cells, and extracellular matrix. Hyaluronic acid (HA) and cancer-associated fibroblasts (CAFs) are the major components in the TME and are correlated with PCa proliferation and metastasis, while the underlying mechanism is still not fully understood due to the lack of biomimetic extracellular matrix components and coculture models. In this study, gelatin methacryloyl/chondroitin sulfate-based hydrogels were physically crosslinked with HA to develop a novel bioink for the three-dimensional (3D) bioprinting of a coculture model that can be used to investigate the effect of HA on PCa behaviors and the mechanism underlying PCa-fibroblasts interaction. PCa cells demonstrated distinct transcriptional profiles under HA stimulation, where cytokine secretion, angiogenesis, and epithelial to mesenchymal transition were significantly upregulated. Further coculture of PCa with normal fibroblasts activated CAF transformation, which could be induced by the upregulated cytokine secretion of PCa cells. These results suggested HA could not only promote PCa metastasis individually but also induce PCa cells to activate CAF transformation and form HA-CAF coupling effects to further promote PCa drug resistance and metastasis.

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Section: Ha Induced Caf Transformation Through Pca Cell Cytokine Secr...supporting

confidence: 82%

3D bioprinting of multi-cellular tumor microenvironment for prostate cancer metastasis

Huang

et al. 2023

Biofabrication

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“…74 Moreover, HA has high affinity for the CD44 receptor on the surface of tumor cells, which leads to increasing application of HA hydrogels in cancer therapy. 75 Leng et al fabricated an injectable HA hydrogel encapsulating paclitaxel (PTX) nanoparticles and epirubicin for the treatment of post-operative breast tumors. Benefiting from the properties of good biocompatibility, non-immunogenicity and sustained drug release of HA hydrogels, this drug-loaded hydrogel achieved long-term inhibition of tumor relapse and extended survival time with no toxicity.…”

Section: Hydrogels With Different Skeletonsmentioning

confidence: 99%

Recent advances in hydrogels for preventing tumor recurrence

et al. 2023

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“…Such tunability of various biomaterials allows for the interrogation of cell-matrix interactions and the development of superior in vitro models. For example, composite hydrogels of chitosan and chondroitin sulfate -an extracellular matrix protein found in high abundance in PCa metastases -upregulated epithelialto-mesenchymal transition markers and increased resistance to docetaxel treatment compared to PCa cells grown in 2D culture (Xu et al 2020). Thus, the culture of PCa cells in tunable hydrogels facilitates a cell phenotype more reminiscent of aggressive metastatic PCa, offering a more biologically relevant platform for candidate drug testing.…”

Section: Compositionmentioning

confidence: 99%

Prostate cancer and bone: clinical presentation and molecular mechanisms

Wells

Krackeler

Jathal

et al. 2023

Endocrine-Related Cancer

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Prostate cancer (PCa) is an increasingly prevalent health problem in the developed world. Effective treatment options exist for localized PCa, but metastatic PCa has fewer treatment options and shorter patient survival. PCa and bone health are strongly entwined, as PCa commonly metastasizes to the skeleton. Since androgen receptor signaling drives PCa growth, androgen-deprivation therapy whose sequelae reduce bone strength constitutes the foundation of advanced PCa treatment. The homeostatic process of bone remodeling – produced by concerted actions of bone building osteoblasts, bone resorbing osteoclasts and regulatory osteocytes — may also be subverted by PCa to promote metastatic growth. Mechanisms driving skeletal development and homeostasis, such as regional hypoxia or matrix-embedded growth factors, may be subjugated by bone metastatic PCa. In this way, the biology that sustains bone is integrated into adaptive mechanisms for the growth and survival of PCa in bone. Skeletally metastatic PCa is difficult to investigate due to the intwined nature of bone biology and cancer biology. Herein, we survey PCa from origin, presentation, and clinical treatment to bone composition and structure and molecular mediators of PCa metastasis to bone. Our intent is to quickly yet effectively reduce barriers to team science across multiple disciplines that focuses on PCa and metastatic bone disease. We also introduce concepts of tissue engineering as a novel perspective to model, capture, and study complex cancer-microenvironment interactions.

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Material properties of disulfide-crosslinked hyaluronic acid hydrogels influence prostate cancer cell growth and metabolism

Abstract: Cells reside in vivo within three dimensional environments in which they interact with extracellular matrices (ECMs) that play an integral role in maintaining tissue homeostasis and preventing tumour growth. Thus,...

Cited by 10 publications

References 72 publications

3D bioprinting of multi-cellular tumor microenvironment for prostate cancer metastasis

3D bioprinting of multi-cellular tumor microenvironment for prostate cancer metastasis

Recent advances in hydrogels for preventing tumor recurrence

Prostate cancer and bone: clinical presentation and molecular mechanisms

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