Materials design at the interface of nanoparticles and innate immunity

Szeto, Gregory L.; Lavik, Erin B.

doi:10.1039/c5tb01825k

Cited by 68 publications

(45 citation statements)

References 91 publications

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“…For instance, size, shape, solubility, surface chemistry, and hydrophilicity can be tuned and controlled, which allows the preparation of nanoparticles with tailored biological properties (28). Moreover, nanoparticles can be designed to allow the incorporation of a wide range of molecules including antigens which makes them highly interesting in vaccinology (29,30).…”

Section: Nanoparticles An Alternative Approach To Conventional Vaccinesmentioning

confidence: 99%

Nanoparticle-Based Vaccines Against Respiratory Viruses

et al. 2019

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The respiratory mucosa is the primary portal of entry for numerous viruses such as the respiratory syncytial virus, the influenza virus and the parainfluenza virus. These pathogens initially infect the upper respiratory tract and then reach the lower respiratory tract, leading to diseases. Vaccination is an affordable way to control the pathogenicity of viruses and constitutes the strategy of choice to fight against infections, including those leading to pulmonary diseases. Conventional vaccines based on live-attenuated pathogens present a risk of reversion to pathogenic virulence while inactivated pathogen vaccines often lead to a weak immune response. Subunit vaccines were developed to overcome these issues. However, these vaccines may suffer from a limited immunogenicity and, in most cases, the protection induced is only partial. A new generation of vaccines based on nanoparticles has shown great potential to address most of the limitations of conventional and subunit vaccines. This is due to recent advances in chemical and biological engineering, which allow the design of nanoparticles with a precise control over the size, shape, functionality and surface properties, leading to enhanced antigen presentation and strong immunogenicity. This short review provides an overview of the advantages associated with the use of nanoparticles as vaccine delivery platforms to immunize against respiratory viruses and highlights relevant examples demonstrating their potential as safe, effective and affordable vaccines.

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Section: Nanoparticles An Alternative Approach To Conventional Vaccinesmentioning

confidence: 99%

Nanoparticle-Based Vaccines Against Respiratory Viruses

et al. 2019

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“…In conjunction with this system is the highly organized complement system, which involves a set of serum proteins that circulate in an inactive state. Those proteins are converted into an active state through three pathways (classical, lection and alternative pathway) to damage and clear pathogenic organisms [19]. Activation of the complement system leads to the formation of the potent anaphylatoxins C3a and C5a.…”

Section: Innate Immune Systemmentioning

confidence: 99%

“…APCs and neutrophils are responsible for recognizing pathogens via PRRs, which identify pathogen-associated molecular patterns (PAMPs). Following identification, the cells uptake and digest the pathogen, generating an inflammatory response [19,21]. APCs are also activated by damage-associated molecular pattern molecules (DAMPs) (such as ATP, uric acid, heparin sulfate) from stressed or damaged tissues or microbes [22].…”

Section: Innate Immune Systemmentioning

confidence: 99%

Effects of engineered nanoparticles on the innate immune system

Liu

Hardie

Zhang

et al. 2017

Seminars in Immunology

229

131

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Engineered nanoparticles (NPs) have broad applications in industry and nanomedicine. When NPs enter the body, interactions with the immune system are unavoidable. The innate immune system, a non-specific first line of defense against potential threats to the host, immediately interacts with introduced NPs and generates complicated immune responses. Depending on their physicochemical properties, NPs can interact with cells and proteins to stimulate or suppress the innate immune response, and similarly activate or avoid the complement system. NPs size, shape, hydrophobicity and surface modification are the main factors that influence the interactions between NPs and the innate immune system. In this review, we will focus on recent reports about the relationship between the physicochemical properties of NPs and their innate immune response, and their applications in immunotherapy.

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“…The principle of the method was based on the evaluation of the adsorption of individual proteins and the establishment of a map of surface adsorption/exclusion assumed to reflect the surface coverage property of the tested NM. The choice of proteins to probe surface properties of NM was also consistent with their key role in mechanisms controlling the in vivo activity of these compounds [37,40,41]. The proposed method was designed to provide a simple model evaluating NM surface functionality that can be implemented in routine to be used in quality control of NMs.…”

Section: Introductionmentioning

confidence: 99%

Characterization of nanomedicines’ surface coverage using molecular probes and capillary electrophoresis

Coty

Varenne

Benmalek

et al. 2018

European Journal of Pharmaceutics and Biopharmaceutics

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A faithful characterization of nanomedicine (NM) is needed for a better understanding of their in vivo outcomes. Size and surface charge are studied with well-established methods. However, other relevant parameters for the understanding of NM behavior in vivo remain largely inaccessible. For instance, the reactive surface of nanomedicines, which are often grafted with macromolecules to decrease their recognition by the immune system, is excluded from a systematic characterization. Yet, it is known that a subtle modification of NMs' surface characteristics (grafting density, molecular architecture and conformation of macromolecules) is at the root of major changes in the presence of biological components. In this work, a method that investigates the steric hindrance properties of the NMs' surface coverage based on its capacity to exclude or allow adsorption of well-defined proteins was developed based on capillary electrophoresis. A series of proteins with different molecular weights (MW) were used as molecular probes to screen their adsorption behavior on nanoparticles bearing different molecular architectures at their surface. This novel strategy evaluating to some degree a functionality of NMs can bring additional information about their shell property and might allow for a better perception of their behavior in the presence of biological components. The developed method could discriminate nanoparticles with a high surface coverage excluding high MW proteins from nanoparticles with a low surface coverage that allowed high MW proteins to adsorb on their surface. The method has the potential for further standardization and automation for a routine use. It can be applied in quality control of NMs and to investigate interactions between proteins and NM in different situations.

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Materials design at the interface of nanoparticles and innate immunity

Abstract: Graphical abstract

Cited by 68 publications

References 91 publications

Nanoparticle-Based Vaccines Against Respiratory Viruses

Nanoparticle-Based Vaccines Against Respiratory Viruses

Effects of engineered nanoparticles on the innate immune system

Characterization of nanomedicines’ surface coverage using molecular probes and capillary electrophoresis

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