Maternal and Neonatal Factors Associated with the High Yield of Mononuclear Low-Density/CD34+ Cells from Placental/Umbilical Cord Blood

Abstract

Placental/umbilical cord blood (CB) contains nucleated cells and hematopoietic stem/ progenitor cells (CD34 + cells). However it is difficult to predict the number of nucleated/ CD34 + cells in each CB before cell processing. Despite many previous studies from institutes affiliated with CB banks in metropolitan areas, little information is available regarding the characteristics of CB units from other medical facilities. The purpose of the present study was to analyze the maternal/neonatal factors on the yield of cells in CB units. A total of 176 CB units were obtained from single-birth and normal vaginal deliveries. Mononuclear low-density (LD) cells were separated using Ficoll-Paque within 24 hrs after CB collection and then processed for the purification of CD34 + cells. A multiple linear regression analysis was performed to assess the correlations between the yield of cells and maternal/neonatal factors including maternal age, gravid status, duration of labor, gestational age, neonatal height and weight, cord length, and meconium in the amniotic fluid. The total LD cells per CB unit had a weak positive correlation with the maternal age of primigravidae. The total LD cells per CB unit from the primigravidae aged 25 were significantly higher than those from the primigravidae aged 24. The total CD34 + cells per CB unit from the 1-gravidae were significantly higher than those from the 2-gravidae and 3-gravidae, respectively among all donors. These results indicate that the CB units from the primigravidae aged 25 are more likely to contain higher yield of LD/CD34 + cells. cord blood; mononuclear cells; CD34 + cells; maternal/neonatal factors.Tohoku

Hematopoietic Stem Cells in Neonates: Any Differences between Very Preterm and Term Neonates?

“…As described by others [16], [24], [26], maternal age had no impact on the HSPC concentration. In contrast, other groups reported an influence of maternal age on HSPC concentration, which is in line with our results [19], [27].…”

The impact of fetal and maternal physiologic factors on umbilical cord blood quality as a source of stem cells in Egyptian population

“…This also was in line with Surbek et al who reported that the CD34+ cell count per cord blood sample was independent of gestational age. On the other hand, Omori et al had found that total number of CD34+ cell had a reverse correlation with the gestational age. Mancinelli et al reported that gestational age of more than 39 weeks increases CD34 significantly.…”

Associated factors of umbilical cord blood collection quality

“…Regarding maternal height, three articles show no correlation with TNC or CD34+ cell quantities in UCB. Concerning UCB volume, data seem controversial . Maternal weight on admission significantly influences CD34+ cells amount as shown in the study by Al‐Sweedan and coworkers, but not in the studies by Omori and coworkers .…”

“…Concerning UCB volume, data seem controversial . Maternal weight on admission significantly influences CD34+ cells amount as shown in the study by Al‐Sweedan and coworkers, but not in the studies by Omori and coworkers . Concerning TNCs, the findings from all these articles are contradictory .…”

“…Maternal age at delivery has been analyzed in 28 of 71 articles . In most articles, no significant correlation was found with UCB volume, TNCs, CD34+ cells, or CFU (Fig.…”