Maternal dietary 22: 6n-3 is more effective than 18: 3n-3 in increasing the 22: 6n-3 content in phospholipids of glial cells from neonatal rat brain

Bowen, Raffick A.R.; Clandinin, Michael T.

doi:10.1079/bjn20041390

Cited by 61 publications

(51 citation statements)

References 133 publications

(122 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Increased availability of DHA increases PtdCho synthesis in fetal lung explants by a direct stimulatory effect of DHA on the rate-limiting enzyme in the pathway, CTP: choline cytidylyltransferase (42), and if the same effect occurs in fetal brain, it may contribute to the changes in PtdCho-DHA that we report. Other studies reported that increasing maternal dietary DHA increased DHA content of PtdEtn and PtdSer of whole brain glial cells in rat pups (43). We found this to be true, along with similar effects on PtdIns-DHA, in fetal brains from the Pemt Ϫ/Ϫ pups whose dams were fed a diet supplemented with DHA (Tables 1 and 2).…”

Section: Discussionsupporting

confidence: 66%

Dietary Docosahexaenoic Acid Supplementation Modulates Hippocampal Development in the Pemt−/− Mouse

Costa

Kiranmai

Craciunescu

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

The development of fetal brain is influenced by nutrients such as docosahexaenoic acid (DHA, 22:6) and choline. Phosphatidylethanolamine-N-methyltransferase (PEMT) catalyzes the biosynthesis of phosphatidylcholine from phosphatidylethanolamine enriched in DHA and many humans have functional genetic polymorphisms in the PEMT gene. Previously, it was reported that Pemt ؊/؊ mice have altered hippocampal development. The present study explores whether abnormal phosphatidylcholine biosynthesis causes altered incorporation of DHA into membranes, thereby influencing brain development, and determines whether supplemental dietary DHA can reverse some of these changes. Pregnant C57BL/6 wild type (WT) and Pemt ؊/؊ mice were fed a control diet, or a diet supplemented with 3 g/kg of DHA, from gestational day 11 to 17. Brains from embryonic day 17 fetuses derived from Pemt ؊/؊ dams fed the control diet had 25-50% less phospholipid-DHA as compared with WT (p < 0.05). Also, they had 60% more neural progenitor cell proliferation (p < 0.05), 60% more neuronal apoptosis (p < 0.01), and 30% less calretinin expression (p < 0.05; a marker of neuronal differentiation) in the hippocampus compared with WT. The DHA-supplemented diet increased fetal brain Pemt ؊/؊ phospholipid-DHA to WT levels, and abrogated the neural progenitor cell proliferation and apoptosis differences. Although this diet did not change proliferation in the WT group, it halved the rate of apoptosis (p < 0.05). In both genotypes, the DHA-supplemented diet increased calretinin expression 2-fold (p < 0.05). These results suggest that the changes in hippocampal development in the Pemt ؊/؊ mouse could be mediated by altered DHA incorporation into membrane phospholipids, and that maternal dietary DHA can influence fetal brain development.

show abstract

Section: Discussionsupporting

confidence: 66%

Dietary Docosahexaenoic Acid Supplementation Modulates Hippocampal Development in the Pemt−/− Mouse

Costa

Kiranmai

Craciunescu

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…DHA may be synthesised from its precursor, a-linolenic acid (LNA) via eicosapentaenoic acid (EPA), and is thought to be selectively transported across the placenta (Haggarty et al, 1997;Dutta-Roy, 2000). It remains unclear whether endogenous synthesis of DHA is always adequate to meet the needs of the developing neonatal brain (Bowen and Clandinin, 2005).…”

Section: Introductionmentioning

confidence: 99%

Neonatal lamb vigour is improved by feeding docosahexaenoic acid in the form of algal biomass during late gestation

Pickard

Beard

Seal

et al. 2008

Animal

View full text Add to dashboard Cite

To determine whether feeding a sustainable, algal source of docosahexaenoic acid (DHA) to sheep during late pregnancy would improve neonatal lamb vigour, 48 English mule ewes, of known conception date, were divided into four treatment groups. For the last 9 weeks of gestation, ewes received one of two dietary supplements: either a DHA-rich algal biomass providing 12 g DHA/ewe per day, or a control supplement based on vegetable oil. The four dietary treatment groups (n 5 12) were: control supplement for the duration of the trial (C), DHA supplement from 9 to 6 weeks before parturition (3 week), DHA supplement from 9 to 3 weeks before parturition (6 week) and DHA supplement for the duration of the trial (9 week). Dietary supplements were fed alongside grass silage and commercial concentrate. There was a tendency for gestation length to be extended with increased duration of DHA supplementation (P 5 0.08). After parturition, the concentrations of eicosapentaenoic acid (EPA) and DHA in ewe and lamb plasma and colostrum were elevated in line with increased periods of DHA supplementation. Lambs from the 6-week and 9-week groups stood significantly sooner after birth than lambs from the C group (P , 0.05). These data show that neonatal vigour may be improved by the supplementation of maternal diets with DHA-rich algal biomass and that this beneficial effect depends upon the timing and/or duration of DHA allocation.

show abstract

“…Ixtaina et al (2011) relatam que a incorporação de sementes de chia na dieta seria benéfico, devido ao elevado teor de AGP presentes na sua composição (9,2%). De acordo com Bowen et al (2005) o consumo de óleos com elevados teores de AGPs pode fornecer benefícios a saúde, além de tornar o óleo mais estável). O óleo extraído das sementes de chia apresentou uma relação AGPI: AGS >11, considerado adequado para alimentos (Matsushita et al, 2010;Ramos-Filho et al, 2008;França et al, 2011).…”

Section: Quantificação De áCidos Graxos Totaisunclassified

Avaliação Do Efeito Das Variáveis De Processo Na Obtenção Do Óleo De Sementes De Chia Por Extração Assistida Por Ultrassom

Mello¹,

Silva²,

Silva³

2015

Anais Do XX Congresso Brasileiro De Engenharia Química

View full text Add to dashboard Cite

RESUMO -O presente trabalho teve como objetivo avaliar o efeito das variáveis de processo na condução da extração assistida por ultrassom do óleo de sementes de chia, bem como o perfil de ácidos graxos do extrato obtido. Os experimentos avaliam diferentes solventes para extração, sendo que os rendimentos obtidos com n-hexano e acetato de etila não apresentam diferenças significativas (p>0,05). Na avaliação do efeito das variáveis de processo os resultados obtidos, com acetato de etila como solvente, relatam que a quantidade de solvente utilizado da extração apresenta-se como a variável com maior efeito significativo (p<0,05), dentro da faixa experimental avaliada, seguida da temperatura. O maior rendimento em óleo foi obtido em 60 ºC, 40 minutos de extração e razão massa de sementes: volume de solvente 1:12. O perfil de ácidos graxos do extrato reporta predominância do ácido linolênico, sendo que o mesmo apresenta similar aos extratos obtidos por outros métodos de extração. INTRODUÇÃONos últimos anos as sementes de chia foram incluídas na dieta humana devido a sua importante composição e os benefícios que o seu consumo traz à saúde (Ixtaina et al., 2011;Capitani et al., 2012). De acordo com Ixtaina et al. (2011), a chia tem sido investigada e recomendada para o consumo, devido ao seu elevado percentual de ácidos graxos benéficos para saúde, proteínas, compostos antioxidantes e fibras alimentares. A literatura relata a adição de chia na formulação de diversos produtos alimentícios (Maruyama et al., 2013, Pizarro et al., 2013.De acordo com Borneo et al. (2010) de 25% a 35% da composição da chia é óleo, principalmente ácidos graxos essenciais (ácido linoleico e linolênico), além de ~22% de fibras e ~24 % de proteínas. Ayerza (1995) e Dauksas et al. (2002) reportam que o óleo de sementes de chia é um produto com composição química variável, dependendo de vários fatores como o ambiente de cultivo e o sistema de extração. Capitani et al. (2012) relataram que a preparação da matéria-prima e diferentes tipos de solventes utilizados para extração do óleo podem afetar de forma diferente o seu conteúdo. Martínez et al. (2012) relataram que um dos principais objetivos na produção do óleo de chia é encontrar um método adequado para preservar a qualidade do óleo.A extração assistida por ultrassom é considerada um método não convencional que vem demonstrando eficiência na extração de compostos bioativos, trazendo vantagens relacionadas ao menor consumo de solventes, baixos tempos de extração e elevados rendimentos de extração quando comparado aos métodos tradicionais (Chemat et al., 2011;Vilkhu et al., 2008). A presente técnica de extração é fundamentada no fenômeno de cavitação (Esclapez et al., 2011), que Área temática: Engenharia e Tecnologia de Alimentos 1

show abstract

Maternal dietary 22: 6n-3 is more effective than 18: 3n-3 in increasing the 22: 6n-3 content in phospholipids of glial cells from neonatal rat brain

Cited by 61 publications

References 133 publications

Dietary Docosahexaenoic Acid Supplementation Modulates Hippocampal Development in the Pemt−/− Mouse

Dietary Docosahexaenoic Acid Supplementation Modulates Hippocampal Development in the Pemt−/− Mouse

Neonatal lamb vigour is improved by feeding docosahexaenoic acid in the form of algal biomass during late gestation

Avaliação Do Efeito Das Variáveis De Processo Na Obtenção Do Óleo De Sementes De Chia Por Extração Assistida Por Ultrassom

Contact Info

Product

Resources

About