Maternal immunization with ovalbumin or Dermatophagoides pteronyssinus has opposing effects on FcγRIIb expression on offspring B cells

Abstract

BackgroundOver the last decade, our group has demonstrated that murine preconception immunization with allergens has a protective effect on allergy development in offspring. The murine model used in the present study allowed us to compare allergy induction by ovalbumin (OVA) and dust mite extract from Dermatophagoides pteronyssinus (Dp).FindingsFemale mice were immunized with OVA or Dp. Pups from immunized and non-immune mothers were immunized at 3 days old (do) with the same antigen used for the maternal immunization. The offspring were analyzed at 20 do. Preconceptional immunization with OVA or Dp did not increase maternal IgE serum levels, although the immunizations induced an increase in allergen-specific IgG1 Ab levels. Offspring serum analyses revealed that maternal immunization with OVA suppressed IgE production only in offspring immunized with OVA. Both preconception immunization protocols inhibited cellular influx into the airways of immunized offspring compared with controls. Similar frequencies of offspring IgM + B cells were found in the OVA- and Dp-immunized groups compared with their respective control groups. Moreover, preconception immunization with OVA enhanced FcγRIIb expression on OVA-immunized offspring B cells. In contrast, decreased FcγRIIb expression was detected on Dp-immunized offspring B cells compared with cells from the offspring of non-immune mothers.ConclusionsTogether, these results show that preconception OVA immunization and Dp immunization can inhibit allergy development but have opposite effects on FcγRIIb expression on offspring B cells.

Preconception allergen sensitization can induce B10 cells in offspring: a potential main role for maternal IgG

“…The maternal immunization protocol with OVA suppressed the offspring allergic response, sensitizing the offspring at a similar humoral level as previously observed by our laboratory in a similar mouse model [5, 13]. The protection induced by maternal immunization in murine models was previously evidenced by other researchers [15].…”

“…2 c). Our laboratory previously reported that the maternal Dermatophagoides pteronyssinus (Dp) immunization protocol induces an opposite effect on FcγRIIB expression in offspring B cells compared with OVA [ 13 ], and we thus evaluated the induction of B10 cells in this model. Even at a higher dose of allergen than that used in our previous experiments, maternal Dp immunization did not induce B10 cells in offspring, and the opposite effect on FcγRIIB expression was maintained (Fig.…”

“…The OVA-specific IgG1, IgM and total IgE antibodies were measured by ELISA as previously described [ 13 ]. To measure the total IgE level, a standard curve was used (Pharmingen, USA).…”

Preconception immunization can modulate intracellular Th2 cytokine profile in offspring: in vivo influence of interleukin 10 and B/T cell collaboration

“…Our group has already reported that the maternal immunization protocol with OVA in WT mice could down-regulate the intracellular IL-4 production on offspring, suggesting a Th2 inhibition [14], and four years later, we also observed that maternal immunization with a dust mite extract ( Dermatophagoides pteronyssinus ) could not induce the same cytokine and phenotype profile in offspring cells [7].…”

IgG from atopic dermatitis patients induces IL‐17 and IL‐10 production in infant intrathymic TCD4 and TCD8 cells

“…In the last 15 years, our group has investigated adoptive maternal–fetal immune interactions in the context of allergy regulation . In 2014, the authors suggested that maternal immunization could influence intrathymic maturation of lymphocytes on offspring .…”