Maternal Nanos‐Dependent <scp>RNA</scp> Stabilization in the Primordial Germ Cells of <i>Drosophila</i> Embryos

Sugimori, Seiko; Kumata, Yuji; Kobayashi, Satoru

doi:10.1111/dgd.12414

Cited by 10 publications

(6 citation statements)

References 43 publications

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“…Germline lineage specification, expansion of the gamete precursors (PGCs), meiosis, and terminal differentiation into functional gametes are all elaborate processes that require extensive regulation of gene expression 1,2 . Together with unique transcriptional and epigenetic mechanisms, regulation of translation plays a critical role in the differentiation of germ cells [3][4][5] .…”

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confidence: 99%

The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation

et al. 2020

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Deleted in azoospermia-like (DAZL) is an RNA-binding protein critical for gamete development. In full-grown oocytes, the DAZL protein increases 4-fold during reentry into the meiotic cell cycle. Here, we have investigated the functional significance of this accumulation at a genome-wide level. Depletion of DAZL causes a block in maturation and widespread disruption in the pattern of ribosome loading on maternal transcripts. In addition to decreased translation, DAZL depletion also causes translational activation of a distinct subset of mRNAs both in quiescent and maturing oocytes, a function recapitulated with YFP-3′UTR reporters. DAZL binds to mRNAs whose translation is both repressed and activated during maturation. Injection of recombinant DAZL protein in DAZL-depleted oocytes rescues the translation and maturation to MII. Mutagenesis of putative DAZL-binding sites in these mRNAs mimics the effect of DAZL depletion. These findings demonstrate that DAZL regulates translation of maternal mRNAs, functioning both as the translational repressor and activator during oocyte maturation.

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confidence: 99%

The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation

et al. 2020

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“…Fly strains used in this study were as follows: y w, vasa-EGFP 18 , nanos-Gal4-VP16 ( nos-Gal4 ) 8 , and UASp-EGFP-K10 3´UTR ( UASp-EGFP ). The UASp-EGFP transgene is in the same attP site as the UASt- and UASz-EGFP transgenes 19 . UASt-Redstinger ( UASt-RFP I) (stock No.…”

Section: Methodsmentioning

confidence: 99%

Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila

Masukawa

Ishizaki

Miura

et al. 2021

Sci Rep

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In Drosophila, three types of UAS vectors (UASt, UASp, and UASz) are currently available for use with the Gal4-UAS system. They have been used successfully in somatic cells and germline cells from ovaries. However, it remains unclear whether they are functional in the germline cells of embryos, larvae, and adult testes. In this study, we found that all three types of UAS vectors were functional in the germline cells of embryos and larvae and that the UASt and UASz vectors were active in the germline of the distal tip region in adult testes. Moreover, we observed that protein expression from the UAS vectors was male-biased in germline cells of late embryos, whereas their respective mRNA expression levels were not. Furthermore, O-propargyl-puromycin (OPP) staining revealed that protein synthesis was male-biased in these germline cells. In addition, GO terms related to translation and ribosomal maturation were significantly enriched in the male germline. These observations show that translational activity is higher in male than in female germline cells. Therefore, we propose that male-biased protein synthesis may be responsible for the sex differences observed in the early germline.

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“…Data shown in figures were obtained from one of the two independent transformant lines, as we found no significant difference between the two lines. To establish UASp-impα2 WT and UASp-impα2 ΔNRE flies, germline transformation was performed using embryos derived from y vas-φ-zh2A w ; ZH-attP-58A females [72], and a single transformant line was established for each transgene, as described previously [76].…”

Section: Methodsmentioning

confidence: 99%

Maternal Nanos inhibits Importin-α2/Pendulin-dependent nuclear import to prevent somatic gene expression in the Drosophila germline

et al. 2019

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Repression of somatic gene expression in germline progenitors is one of the critical mechanisms involved in establishing the germ/soma dichotomy. In Drosophila , the maternal Nanos (Nos) and Polar granule component (Pgc) proteins are required for repression of somatic gene expression in the primordial germ cells, or pole cells. Pgc suppresses RNA polymerase II-dependent global transcription in pole cells, but it remains unclear how Nos represses somatic gene expression. Here, we show that Nos represses somatic gene expression by inhibiting translation of maternal importin-α2 ( impα2 ) mRNA. Mis-expression of Impα2 caused aberrant nuclear import of a transcriptional activator, Ftz-F1, which in turn activated a somatic gene, fushi tarazu ( ftz ), in pole cells when Pgc-dependent transcriptional repression was impaired. Because ftz expression was not fully activated in pole cells in the absence of either Nos or Pgc, we propose that Nos-dependent repression of nuclear import of transcriptional activator(s) and Pgc-dependent suppression of global transcription act as a ‘double-lock’ mechanism to inhibit somatic gene expression in germline progenitors.

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Maternal Nanos‐Dependent RNA Stabilization in the Primordial Germ Cells of Drosophila Embryos

Cited by 10 publications

References 43 publications

The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation

The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation

Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila

Maternal Nanos inhibits Importin-α2/Pendulin-dependent nuclear import to prevent somatic gene expression in the Drosophila germline

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