Maternal Oxidized Soybean Oil Administration in Rats during Pregnancy and Lactation Alters the Intestinal DNA Methylation in Offspring

Abstract

As a food contaminant, oxidized oil or lipid oxidative products have been proven to exert toxicological effects on the growth and development of animals and humans. Research shows that maternal oxidative stress damage might transmit to another generation by epigenetic modulation. However, current evidence is still not clear on the mechanism of the effects of dietary oxidized oil during pregnancy on the two generations. This study employed a rat model fed with oxidized soybean oil (OSO) during pregnancy and lactation to explore the effects of the oxidative degree (0, 200, 400, and 800 mequiv of O 2 /kg) on the placental RNA methylation and DNA methylation in offspring jejunum. The results showed that following the ingestion of OSO, the placental genes of different m 6 A methylation were significantly enriched to nutrient metabolic processes and hormone activity. In addition, the intestine in offspring hypofunctioned observably, such as reducing the height of villi and the level of anti-inflammatory cytokine. Furthermore, maternal intake of OSO during pregnancy can damage the intestinal barrier function of offspring by inhibiting the proliferation and differentiation of intestinal epithelial cells and reducing the activity of intestinal DNA methyltransferase. In conclusion, this study reinforces the assertion that maternal OSO consumption during gestation and lactation negatively affects the placental health and intestinal development of suckling pups.

Oxidized Soybean Oil Evoked Hepatic Fatty Acid Metabolism Disturbance in Rats and their Offspring

“…Previously, our study showed that maternal exposure to OSO resulted in damage to the placenta and offspring intestinal. , However, very few studies have evaluated the effect of OSO on the liver metabolism of fatty acids in maternal and their offspring. In the liver, oxidized lipids accumulate at the highest level .…”

“…POV was examined in previous research. 20 Experimental Animals and Designs. A total of sixty female Sprague−Dawley (SD) rats were purchased from Liaoning Changsheng Biotechnology Co., Ltd (Shenyang, China), with the animal permit number SCXK (Liao) 2015-0001.…”

“…The specific experimental design is shown in Figure . Diet composition and nutritional standards are as we have previously described . In addition, the Institutional Animal Care and Use Committee of Northeast Agricultural University approved all experimental procedures (certification number NEAU- [2011]-9).…”

N6-methyladenosine modifications in maternal-fetal crosstalk and gestational diseases

“…However, the mRNA expression levels of Mettl3 , Mettl14, Alkbh5 and Fto are decreased in placentas after OSO ingestion, with a lower global m 6 A level. The placental genes with differential m 6 A levels are related to nutrient metabolic process and hormone activity ( Wang et al, 2022b ). In addition, exposure to BPDE [benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide], the metabolite of Benzo(a)pyrene (BaP) which is one representative of PAHs (polycyclic aromatic hydrocarbons), upregulates the expression level of m 6 A-modified lnc-HZ01 and inhibits the proliferation of trophoblast cells.…”

“…Epigenetics is a possible link between the environment and fetal growth, and RNA m 6 A is also inherited across generations. Maternal ingestion of oxidized soybean oil (OSO) during gestation and lactation disturbs the homeostasis of DNA/RNA methylation and negatively affects the placental function and intestinal development in offspring, with a reduced height of villi and a lower level of anti-inflammatory factors ( Wang et al, 2022b ). In addition, maternal engineered nanomaterial (ENM) exposure increases the m 6 A level in the 3′UTR region of mitochondria phospholipid hydroperoxide glutathione peroxidase (mPHGPx) which is an antioxidant enzyme that protects fetal cells from oxidative stress.…”