MATH domain proteins represent a novel protein family in Arabidopsis thaliana, and at least one member is modified in roots during the course of a plant–microbe interaction

Abstract

The basidiomycete Piriformospora indica interacts with Arabidopsis roots and mimics an arbuscular mycorrhiza. A MATH [meprin and TRAF (tumour necrosis factor receptor‐associated factor) homology] domain‐containing (MATH) protein at the plasma membrane of Arabidopsis roots is one of the first components to respond to the presence of this fungus. MATH proteins are involved in nodule formation in Medicago and protein degradation in the Arabidopsis cytosol. They exhibit sequence similarities to meprins, extracellular peptidases which cleave (signal) peptides, and to TRAFs, intracellular proteins which interact with receptor kinases at the plasma membrane. Fifty‐nine genes for MATH proteins are present in the Arabidopsis genome. Members of this protein family are predicted to be found in the ER–plasma membrane–extracellular space continuum, in the nucleus–cytosol compartment and in organelles. In this article, we describe this novel class of plant genes. We also use MS‐MS analyses to identify the subcellular localization of individual members of the MATH protein family in Arabidopsis thaliana.

TRAF Family Proteins Regulate Autophagy Dynamics by Modulating AUTOPHAGY PROTEIN6 Stability in Arabidopsis

“…The Arabidopsis genome contains more than 70 genes encoding TRAF domain-containing proteins (Oelmüller et al, 2005;Huang et al, 2016), but few of their functions have been characterized to date. To isolate TRAF family proteins with potential roles in autophagy, we cloned half of the TRAF family genes and fused them to GFP for transient expression in Arabidopsis protoplasts.…”

Monodehydroascorbate reductase 2 and dehydroascorbate reductase 5 are crucial for a mutualistic interaction between Piriformospora indica and Arabidopsis

“…Verma et al, 1998;Sahay and Varma, 1999;Varma et al, 1999Varma et al, , 2001Pham et al, 2004;Peškan-Berghöfer et al, 2004;Oelmüller et al, 2005;Shahollari et al, 2005Shahollari et al, , 2007Sherameti et al, 2005Sherameti et al, , 2008aWaller et al, 2005). Hyphae can be detected on the root surface, in the outer cell layers of the roots and within the root cells, but do not form arbuscular structures in the plant cells (Peškan-Berghöfer et al, 2004).…”

The Role of Auxins and Cytokinins in the Mutualistic Interaction Between Arabidopsis and Piriformospora indica

“…The endophytic fungus Piriformospora indica, a member of the Sebacinaceae family, colonizes the roots of many plant species including mono-and dicots and mosses, many of which are of forestry, agricultural, horticultural, and medicinal importance (Barazani et al 2005;Glen et al 2002;Oelmüller et al 2005;Peškan-Berghöfer et al 2004;Pham et al 2004;Sahay and Varma 1999;Selosse et al 2002a and b;Shahollari et al 2005;Sherameti et al 2005, 2008a andUrban et al 2003;Varma et al 1999Varma et al , 2001Waller et al 2005;Weiss et al 2004). Root colonization is accompanied by the promotion of growth and higher seed yield, and the plants are more resistant to various biotic and abiotic stresses Rai et al 2004;Sherameti et al 2008a and b;Varma et al 2001;Waller et al 2005).…”