Mathematical analysis of catabolic function loss in a population of Pseudomonas putida mt-2 during non-limited growth on benzoate

Duetz, Wouter A.; Winson, Michael K.; Andel, J. van; Williams, Peter

doi:10.1099/00221287-137-6-1363

Cited by 28 publications

(22 citation statements)

References 25 publications

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“…Cell debris and excretion products of TOL+ cells probably support growth of the TOL-population. This phenomenon may possibly be related to the inhibitory effect of benzoate on TOL+ cells described in the preceding paper (Duetz et a/., 1991).…”

Section: Discussionmentioning

confidence: 88%

“…The nomenclature and symbols used in this paper are the same as in the preceding paper (Duetz et al, 1991); in addition, Cis the ratio of the growth rates of the TOL+ and TOL-populations and t g 5 % is defined as the time at which the percentage of TOL+ cells falls below 95%.…”

Section: Methodsmentioning

confidence: 99%

“…Air was supplied through the same needle at 100 ml min-'. The fermenter was equipped with two stainless steel baffles and was kept at 28 "C. P. putida mt-2 was grown on succinate at a non-limiting rate by making use of a phauxostat as described in the preceding paper (Duetz et al, 1991). Whenever wall growth was observed, the complete cell suspension was transferred to a new sterile fermenter vessel of the same type, without interrupting the addition of medium for more than 1 min.…”

Section: Methodsmentioning

confidence: 99%

“…For persistence experiments in phauxostat culture a value for C was calculated from the value for the growth rates of the TOL+ and TOLcells as determined according to the preceding paper (Duetz et al, 1991).…”

Section: Methodsmentioning

confidence: 99%

“…Assays for cell density, the percentage of TOL+ cells in a population, the statistical calculation of 95 % reliability intervals and the genetic characterization of TOL-cells were done as described in the preceding paper (Duetz et al, 1991).…”

Section: Methodsmentioning

confidence: 99%

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Stability of TOL plasmid pWW0 in Pseudomonas putida mt-2 under non-selective conditions in continuous culture

Duetz

Andel²

1991

Journal of General Microbiology

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Pseudomonusputidu mt-2, harbouring the TOL plasmid pW WO, was grown in chemostat culture under succinate-, sulphate-, ammonium-or phosphate-limitation at different dilution rates. The fraction of mutant cells lacking the plasmid-encoded enzymes for the degradation of toluene and xylene (TOL-cells), was determined. Genetic analysis revealed that all TOL-cells isolated harboured partially deleted plasmids, lacking the TOL catabolic genes. The growth-rate advantage of the TOL-cells was quantified from the kinetics of their increase as a fraction of the total population. At a dilution rate of 0.1 h-l no growth-rate advantage of TOL-cells was found when phosphate or ammonium were limiting. Under sulphate-limitation, ingrowth of TOL-cells was evident but did not follow a straightforward pattern. Under succinate-limitation the growth-rate advantage was the highest, particularly at low dilution rates (about 50% at D = 0-05 h-l). In phauxostat culture, at the maximal growth rate, the growth-rate advantage of TOL-cells was less than 1 %. The specific activity in TOL+ cells of the plasmidencoded enzyme catechol 2,3-dioxygenase was relatively high at a low growth rate.

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Section: Discussionmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Stability of TOL plasmid pWW0 in Pseudomonas putida mt-2 under non-selective conditions in continuous culture

Duetz

Andel²

1991

Journal of General Microbiology

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Effect of vapor-phase bioreactor operation on biomass accumulation, distribution, and activity: Linking biofilm properties to bioreactor performance

Song¹,

Kinney²

2000

Biotechnol. Bioeng.

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Excess biomass accumulation and activity loss in vapor‐phase bioreactors (VPBs) can lead to unreliable long‐term operation. In this study, temporal and spatial variations in biomass accumulation, distribution and activity in VPBs treating toluene‐contaminated air were monitored over a 96‐day period. Two laboratory‐scale bioreactors were subjected to a toluene loading rate of 45.8 g/m3‐h with one VPB operating in a unidirectional (UD) mode and a second identical VPB operating in a directionally switching (DS) mode. In the UD bioreactor, the contaminated air stream was continuously fed to the bottom of the reactor, while, in the DS bioreactor, the direction of the contaminated gas flow was reversed every three days. Overall, the DS system performed better with respect to biomass distribution and microbial activity across the bioreactor, resulting in more stable bioreactor performance. In contrast, most of the biomass accumulation and activity was confined to the front half of the UD bioreactor column which caused high pressure drops, rapid activity loss and eventually toluene breakthrough. A carbon balance reveals that excess biomass accumulated continuously in both bioreactors, and biomass yield coefficients were very similar (0.59 g dry biomass/g toluene for the UD and 0.63 g dry biomass/g toluene for the DS). The viable biomass population remained relatively constant in both bioreactors over the operational period, while the inactive biomass fraction steadily increased over the same time frame. Biodegradation activity determined by the dehydrogenase enzyme activity assay was found to be a function of biomass accumulation and reflected pollutant removal profiles along the columns. In addition, biomass activity correlated well with the toluene‐degrading fraction of the total bacterial population. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 508–516, 2000.

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Estimating the Instability Parameters of Plasmid-Bearing Cells. I. Chemostat Culture

Ganusov

Brilkov

2002

Journal of Theoretical Biology

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Mathematical analysis of catabolic function loss in a population of Pseudomonas putida mt-2 during non-limited growth on benzoate

Cited by 28 publications

References 25 publications

Stability of TOL plasmid pWW0 in Pseudomonas putida mt-2 under non-selective conditions in continuous culture

Stability of TOL plasmid pWW0 in Pseudomonas putida mt-2 under non-selective conditions in continuous culture

Effect of vapor-phase bioreactor operation on biomass accumulation, distribution, and activity: Linking biofilm properties to bioreactor performance

Estimating the Instability Parameters of Plasmid-Bearing Cells. I. Chemostat Culture

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