Mathematical and numerical challenges in optical screening of female breast

Causin, Paola; Lupieri, Marina G.; Naldi, Giovanni; Weishaeupl, R.M.

doi:10.1002/cnm.3286

Cited by 5 publications

(3 citation statements)

References 35 publications

(53 reference statements)

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“…Setting. We considered synthetic measures generated via the diffusive-reactive forward model (we refer to [9] for details). The phantom is a 2D semidisk with radius 5 cm, discretized in 588 square voxels with side 0.25 cm.…”

Section: Implementation and Numerical Resultsmentioning

confidence: 99%

“…In [7,8] the Elastic-Net regularization term, involving a convex combination of 1 and 2 norms, was investigated for the 2D DOT problem under the Rytov approximation and yielded satisfactorily results. In [9] this approach was validated for the 3D case. As an alternative, [7] presented another regularization strategy, based on Bregman iterations [10,11], which consisted in using the Bregman Divergence.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Learned-SVD approach for Regularization in Diffuse Optical Tomography

Benfenati¹,

Bisazza²,

Causin³

2021

Preprint

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Inverse problems arising in Diffuse Optical Tomography are severely ill-conditioned • Traditional approaches rely on regularization functions with fine tuning of parameters • We explore deep learning techniques in a fully data-driven approach • The approach is the deep learning equivalent of the SVD of a nonlinear functional • The solution is robust even in presence of high levels of noise

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Section: Implementation and Numerical Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Learned-SVD approach for Regularization in Diffuse Optical Tomography

Benfenati¹,

Bisazza²,

Causin³

2021

Preprint

Self Cite

View full text Add to dashboard Cite

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“…Optical imaging (OI) refers to a variety of techniques using either near-infrared (NIR-I & NIR-II, 700–1700 nm)/short-wave infrared (SWIR, 1000–2000 nm) or visible (400–700 nm) light, to provide molecular, morphologic, and functional information, probing absorption, scattering, and fluorescence properties of cells or tissues ( 26 ). The advantages of OI include real-time imaging capability, cost-effective, portable, non-ionizing, and generally well tolerated by patients ( 26 , 27 ). Application of optical imaging dyes or contrast agent, such as FDA-approved indocyanine green (ICG), has further enhanced the usefulness of optical imaging techniques, providing better detection of relatively deep-seated lesions and better guidance of cancer treatments ( 28 , 29 ).…”

Section: Introductionmentioning

confidence: 99%

Interventional Optical Imaging-Monitored Synergistic Effect of Radio-Frequency Hyperthermia and Oncolytic Immunotherapy

et al. 2022

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PurposeTo develop a new interventional oncology technique using indocyanine green (ICG)-based interventional optical imaging (OI) to monitor the synergistic effect of radiofrequency hyperthermia (RFH)-enhanced oncolytic immunotherapy.Materials and MethodsThis study included (1) optimization of ICG dose and detection time-window for intracellular uptake by VX2 tumor cells; (2) in-vitro confirmation of capability of using ICG-based OI to assess efficacy of RFH-enhanced oncolytic therapy (LTX-401) for VX2 cells; and (3) in-vivo validation of the interventional OI-monitored, intratumoral RFH-enhanced oncolytic immunotherapy using rabbit models with orthotopic liver VX2 tumors. Both in-vitro and in-vivo experiments were divided into four study groups (n=6/group) with different treatments: (1) combination therapy of RFH+LTX-401; (2) RFH alone at 42°C for 30 min; (3) oncolytic therapy with LTX-401; and (4) control with saline. For in-vivo validation, orthotopic hepatic VX2 tumors were treated using a new multi-functional perfusion-thermal radiofrequency ablation electrode, which enabled simultaneous delivery of both LTX-401 and RFH within the tumor and at the tumor margins.ResultsIn in-vitro experiments, taking up of ICG by VX2 cells was linearly increased from 0 μg/mL to 100 μg/mL, while ICG-signal intensity (SI) reached the peak at 24 hours. MTS assay and apoptosis analysis demonstrated the lowest cell viability and highest apoptosis in combination therapy, compared to three monotherapies (P<0.005). In in-vivo experiments, ultrasound imaging detected the smallest relative tumor volume for the combination therapy, compared to other monotherapies (P<0.005). In both in-vitro and in-vivo experiments, ICG-based interventional optical imaging detected a significantly decreased SI in combination therapy (P<0.005), which was confirmed by the “gold standard” optical/X-ray imaging (P<0.05). Pathologic/laboratory examinations further confirmed the significantly decreased cell proliferation with Ki-67 staining, significantly increased apoptotic index with TUNEL assay, and significantly increased quantities of CD8 and CD80 positive cells with immunostaining in the combination therapy group, compared to other three control groups (P<0.005).ConclusionsWe present a new interventional oncology technique, interventional optical imaging-monitored RFH-enhanced oncolytic immunotherapy, which may open new avenues to effectively manage those patients with larger, irregular and unresectable malignancies, not only in liver but also the possibility in other organs.

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Interventional real-time optical imaging guidance for complete tumor ablation

Kan

Zhang

Zhou

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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The aim of this study was to develop an interventional optical imaging (OI) technique for intraprocedural guidance of complete tumor ablation. Our study employed four strategies: 1) optimizing experimental protocol of various indocyanine green (ICG) concentrations/detection time windows for ICG-based OI of tumor cells (ICG cells); 2) using the optimized OI to evaluate ablation-heat effect on ICG cells; 3) building the interventional OI system and investigating its sensitivity for differentiating residual viable tumors from nonviable tumors; and 4) preclinically validating its technical feasibility for intraprocedural monitoring of radiofrequency ablations (RFAs) using animal models with orthotopic hepatic tumors. OI signal-to-background ratios (SBRs) among preablation tumors, residual, and ablated tumors were statistically compared and confirmed by subsequent pathology. The optimal dose and detection time window for ICG-based OI were 100 μg/mL at 24 h. Interventional OI displayed significantly higher fluorescence signals of viable ICG cells compared with nonviable ICG cells (189.3 ± 7.6 versus 63.7 ± 5.7 au, P < 0.001). The interventional OI could differentiate three definitive zones of tumor, tumor margin, and normal surrounding liver, demonstrating significantly higher average SBR of residual viable tumors compared to ablated nonviable tumors (2.54 ± 0.31 versus 0.57 ± 0.05, P < 0.001). The innovative interventional OI technique permitted operators to instantly detect residual tumors and thereby guide repeated RFAs, ensuring complete tumor eradication, which was confirmed by ex vivo OI and pathology. In conclusion, we present an interventional oncologic technique, which should revolutionize the current ablation technology, leading to a significant advancement in complete treatment of larger or irregular malignancies.

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Mathematical and numerical challenges in optical screening of female breast

Cited by 5 publications

References 35 publications

A Learned-SVD approach for Regularization in Diffuse Optical Tomography

A Learned-SVD approach for Regularization in Diffuse Optical Tomography

Interventional Optical Imaging-Monitored Synergistic Effect of Radio-Frequency Hyperthermia and Oncolytic Immunotherapy

Interventional real-time optical imaging guidance for complete tumor ablation

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