Mating‐induced release of granules by the endocrine dorsal body cells of the snail <i>Helisoma duryi</i> (Mollusca)

Saleuddin, A. S. M.; Ashton, Mary Lou; Khan, H.R.

doi:10.1002/jez.1402500212

Cited by 13 publications

(7 citation statements)

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“…The DB are located on the dorsal surface of the cerebral commissure and are known to regulate vitellogenesis and growth and differentiation of female accessory glands. The CDC are two clusters of cerebral neurosecretory cells close to the DB and are known to produce egg-laying hormone (Joosse and Geraerts 1983;Ebberink et al 1985;Schollen and Saleuddin 1986;Saleuddin 1985, 1987;Saleuddin et al 1989).…”

Section: Introductionmentioning

confidence: 99%

The effects of mating on the fine structure of neurosecretory caudodorsal cells in Helisoma duryi (Mollusca)

Khan¹,

Ashton²,

Mukai³

et al. 1990

Can. J. Zool.

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The hermaphroditic snail Helisoma duryi does not lay eggs if raised as a virgin in isolation or when castrated. The fine structure of its neurosecretory caudodorsal cells (CDC), which produce the putative egg-laying hormone, was studied in reproductively inactive virgin and castrated snails and compared with that of the reproductively active mated snails, using electron microscopy, tannic acid incubation technique, and electron microscope autoradiography after injection of [3H]leucine. The CDC of virgin and castrated snails accumulate large numbers of elementary granules and appear synthetically inactive, whereas the CDC of mated snails contain fewer elementary granules and possess features characteristic of increased protein synthesis. The flattened cisternae of the rough endoplasmic reticulum of the CDC of the virgins become swollen 3 h after first mating. In tannic acid incubated tissues, more released granules were seen in CDC from snails 12 h after first mating than in CDC from virgin and castrated snails. Autoradiography showed more silver grains on the CDC from snails 12–48 h after first mating than on those from snails 3–6 h after first mating. It is suggested that, in Helisoma, mating provides a stimulus to the CDC for increased protein synthesis and release of the putative egg-laying hormone.

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Section: Introductionmentioning

confidence: 99%

The effects of mating on the fine structure of neurosecretory caudodorsal cells in Helisoma duryi (Mollusca)

Khan¹,

Ashton²,

Mukai³

et al. 1990

Can. J. Zool.

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“…These characteristics have also been observed in the DB cells of other pulmonate molluscs (Nolte, 1983;Griffond and Vincent, 1985;Saleuddin et al, 1989;Buma et al, 1984;Mounzih et al, 1988). The DB of Lymnaea stagnalis does not abundantly express a peptidergic gene, but they express a gene encoding a P450 analogue, which might suggest steroid synthesis (Teunissen et al, 1992).…”

Section: Discussionmentioning

confidence: 65%

“…The DB cells have general features in common to steroid-secreting gland cells, such as the presence of a large number of mitochondria with longitudinal cristae, abundant lipid droplets and developed SER cisternae. These characteristics have also been described for Helix pomatia (Nolte et al, 1986), Helix aspersa (Wijdenes et al, 1983), Achatina fulica , Theba pisana (Nolte, 1983), Arion rufus (Ezzughayyar and Wattez, 1989), and for the aquatic pulmonates Helisoma duryi (Saleuddin et al, 1989) and Siphonaria pectinata (Saleuddin et al, 1997). Indeed, it was demonstrated that the DB cells of H. pomatia and Lymnaea stagnalis have the capacity to synthesise ecdysteroids (Krusch et al, 1979;Nolte et al, 1986, Mukai andSaleuddin, 1992;Mukai et al, 2001), specifically ecdysone (Nolte et al, 1986).…”

Section: Discussionmentioning

confidence: 78%

“…Steroid hormone-producing gland cells have a high rate of protein synthesis, which is necessary to provide enzymes for steroidogenesis and steroid-binding protein. Proteins could be also involved in 1) intracellular transport of cholesterol or intermediate steroids, as has been described for adrenocortical cells or insect prothoracic glands (Feuilloley andVaudry, 1996, Birkenbeil, 1983), and 2) in the storage of steroid hormones in secretory granules, as suggested for the DB cells of Helisoma duryi (Saleuddin et al, 1989). On the other hand, the simultaneous synthesis, storage and release of steroids and proteins are considered a general principle for some mammalian steroidogenic cells (Skinner, 1991).…”

Section: Discussionmentioning

confidence: 95%

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Ultrastructural analysis of the dorsal body gland of the terrestrial snail Megalobulimus abbreviatus (Becquaert, 1948)

Piva

Gonçalves

2010

Braz. J. Biol.

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The ultrastructure of the reproductive gland, dorsal body (DB), of Megalobulimus abbreviatus was analysed. Electron microscope immunohistochemistry was used to detect FMRFamide-like peptides in the nerve endings within this gland. Nerve backfilling was used in an attempt to identify the neurons involved in this innervation. In M. abbreviatus, the DB has a uniform appearance throughout their supraesophageal and subesophageal portions. Dorsal body cells have several features in common with steroid-secreting gland cells, such as the presence of many lipid droplets, numerous mitochondria with tubular cristae and a developed smooth endoplasmic reticulum cisternae. Throughout the DB in M. abbreviatus numerous axonal endings were seen to be in contact with the DB cells exhibiting a synapticlike structure. The axon terminals contained numerous electron-dense and scanty electron-lucid vesicles. In addition, the DB nerve endings exhibited FMRFamide immunoreactive vesicles. Injection of neural tracer into the DB yielded retrograde labelling of neurons in the metacerebrum lobe of the cerebral ganglia and in the parietal ganglia of the subesophageal ganglia complex. The possibility that some of these retrograde-labelled neurons might be FMRFamidelike neurons that may represent a neural control to the DB in M. abbreviatus is discussed.Keywords: mollusc, endocrine gland, FMRFamide, cerebral ganglia, reproductive control. Análise ultraestrutural da glândula corpo dorsal do caracol terrestre Megalobulimus abbreviatus (Becquaert, 1948) ResumoFoi analisada a ultraestrutura da glândula reprodutiva corpo dorsal (CD) de Megalobulimus abbreviatus. Imunoistoquímica para microscopia eletrônica foi utilizada para detectar peptídeos relacionados ao tetrapeptídeo FMRFamida nas terminações axonais existentes nessa glândula. Foi utilizada marcação neuronal retrógada com o intuito de localizar os neurônios envolvidos nesta inervação. O CD de M. abbreviatus possui um aspecto uniforme em toda sua extensão, tanto na porção supraesofágica como subesofágica. As células do CD possuem várias características de glândulas esteroidogênicas, tais como a presença de inúmeras gotículas lipídicas, numerosas mitocôndrias com cristas tubulares e cisternas bem desenvolvidas de retículo endoplasmático liso. Por toda a extensão do CD de M. abbreviatus foram encontradas numerosas terminações axonais fazendo contatos estruturalmente semelhantes a sinapses com as células do CD. As terminações axonais continham grande número de vesículas eletrodensas e esparsas vesículas eletrolúcidas. As terminações axonais no CD apresentavam vesículas com conteúdo imunorreativo à FMRFamida. A injeção de traçador neural no CD resultou em marcação retrógrada de neurônios no metacérebro dos gânglios cerebrais e nos gânglios parietais do complexo ganglionar subesofágico de M. abbreviatus. É discutida a possibilidade de que estes neurônios identificados por marcação retrógrada possam representar a via de controle neural do CD de M. abbreviatus, cujo mediador químico seria um n...

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“…The dorsal body hormone (DBH), whose origin and chemical nature (peptide and/or steroids) remain to be determinate as they could be different depending on the considered species (Nolte et al 1986;Joosse 1988;Saleuddin et al 1989;Teunissen et al 1992;Garcia et al 1995), is produced from the early juvenile stages (Roubos et al 1988). It regulates cell differentiation of the female accessory sexual organs, the vitellogenesislike process and oocyte maturation, and the synthesis…”

Section: Sexual Polymorphismmentioning

confidence: 99%

Endocrine disruption in aquatic pulmonate molluscs: few evidences, many challenges

2007

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As compared to other groups of aquatic gastropods, documented examples of endocrine disruption in pulmonates are rather limited. This is quite surprising because the endocrine control of physiological functions has been extensively studied in these animals. In the model-species Lymnaea stagnalis, the neurohormonal regulation of reproduction has been thoroughly investigated, and the primary structure of several peptides and receptors involved in endocrine processes has been established. However, the use of this knowledge has been fairly limited in the context of ecotoxicology, to investigate the effects of endocrine-disrupting chemicals. The present review summarizes the main and more recent findings on the neuroendocrine control of reproduction in aquatic pulmonate snails (Basommatophora). It then comprehensively describes selected in vivo laboratory and semi-field studies which provide evidence for possible endocrine disrupting effects of estrogenic and androgenic test compounds [e.g., ethynylestradiol, methyltestosterone (MT)], and of environmental contaminants [e.g., cadmium (Cd), tributyltin (TBT), and nonylphenol (NP), pesticides]. Finally, challenging perspectives for future research are discussed.

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Mating‐induced release of granules by the endocrine dorsal body cells of the snail Helisoma duryi (Mollusca)

Cited by 13 publications

References 15 publications

The effects of mating on the fine structure of neurosecretory caudodorsal cells in Helisoma duryi (Mollusca)

The effects of mating on the fine structure of neurosecretory caudodorsal cells in Helisoma duryi (Mollusca)

Ultrastructural analysis of the dorsal body gland of the terrestrial snail Megalobulimus abbreviatus (Becquaert, 1948)

Endocrine disruption in aquatic pulmonate molluscs: few evidences, many challenges

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