Matrices for tissue engineering-scaffold structure for a bioartificial liver support system

Mayer, Joerg; Karamuk, Erdal; Akaike, Takaaki; Wintermantel, E.

doi:10.1016/s0168-3659(99)00136-4

Cited by 75 publications

(33 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microstructure and conformational features of scaffolds have been demonstrated to govern level of differentiation and hepatocyte aggregate behavior in culture [7,8]. We have earlier demonstrated the suitability of textile scaffolds in inducing hepatocyte aggregate formation [9,10]. However, the PLGA matrix used lead to degradation products that are acidic in nature and could lead to undesirable pH changes in cellular microenvironment in long-term organotypic cultures [11].…”

Section: Introductionmentioning

confidence: 99%

Hydrogel-coated textile scaffolds as candidate in liver tissue engineering: II. Evaluation of spheroid formation and viability of hepatocytes

Risbud¹,

Karamuk²,

Schlosser³

et al. 2003

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

In this study we evaluate the performance of primary rat hepatocytes and HepG2 cells on chitosan-collagen hydrogel-coated textile scaffolds. Light microscopy and electron microscopic observations showed attachment and aggregate formation tendency of hepatocytes on the scaffolds. As tested by the tetrazolium reduction (MTT) assay it was evident that cells had preserved mitochondrial functionality. It was also observed that pure collagen and collagen blended scaffolds allowed higher cell growth than pure chitosan scaffold. Fluorescent live/dead staining showed a metabolically active, viable cell population on all scaffold compositions with occurrence of few dead cells. Cell functionality was confirmed by secretion of albumin, which was maintained throughout culture period. Take collectively our results suggests that hydrogel-coated textile scaffolds could be promising for tissue-engineering applications, as they allow favorable hepatocyte attachment, spheroid formation and maintenance of function. These scaffolds could be useful for co-culturing hepatocytes and non-parenchymal endothelial cells in bioartificial liver support systems.

show abstract

Section: Introductionmentioning

confidence: 99%

Hydrogel-coated textile scaffolds as candidate in liver tissue engineering: II. Evaluation of spheroid formation and viability of hepatocytes

Risbud¹,

Karamuk²,

Schlosser³

et al. 2003

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

show abstract

“…In analogy cells are cultured on an artificial extracellular matrix in tissue engineering. [7][8][9] In vitro development of functional tissues as used for cartilage repair or for the development of liver modules can only be expected when both components interact in an optimal manner. The goal in tissue engineering is to generate tissue specific features while avoiding atypical protein expression, caused by suboptimal culture conditions and cellular dedifferentiation.…”

Section: Introduction Tmentioning

confidence: 99%

Proliferating Cells versus Differentiated Cells in Tissue Engineering

et al. 2002

View full text Add to dashboard Cite

The efficiency of cell or tissue cultures is usually judged by how quickly confluence is reached within a Petri dish or on a scaffold. Growth factors and fetal bovine serum are employed to drive cultured cells from one mitosis to the next as quickly as possible. The tissue specific interphase is extremely short under these conditions, so that the degree of differentiation desired in tissue engineering cannot be achieved. To reach the goal of functional differentiation in vitro mitosis and interphase must be separated experimentally and tailored to the specific requirements of the cell-type used. This could be achieved by a three step concept for tissue-engineering in vitro as we present here. The expansion phase is followed by a phase in which tissue differentiation is initiated. The final phase serves to express and maintain histotypical differentiation of the generated tissue.

show abstract

“…As an important part of soft tissue engineering, threedimensional scaffolds provide a place for attachment, increase surface area, support a large cell mass, and are capable of shaping specific structures [1][2][3]. Lack of active groups, slow degradation under physiological condition, and the use of organic solvent that prohibits the addition of cell growth factors, limit the use of synthetic polymers [4][5][6], such as polylactic acid (PLA), polyglycolic acid (PGA), and polylactic glycolic acid (PLGA).…”

Section: Introductionmentioning

confidence: 99%

Preparation and cytocompatibility of silk fibroin/chitosan scaffolds

She

Liu

Feng

2009

Front. Mater. Sci. China

View full text Add to dashboard Cite

One challenge in soft tissue engineering is to find an applicable scaffold, not only having suitable mechanical properties, porous structures, and biodegradable properties, but also being abundant in active groups and having good biocompatibility. In this study, a threedimensional silk fibroin/chitosan (SFCS) scaffold was successfully prepared with interconnected porous structure, excellent hydrophilicity, and proper mechanical properties. Compared with polylactic glycolic acid (PLGA) scaffold, the SFCS scaffold further facilitated the growth of HepG2 cells (human hepatoma cell line). Keeping the good cytocompatibility and combining the advantages of both fibroin and chitosan, the SFCS scaffold should be a prominent candidate for soft tissue engineering, for example, liver.

show abstract

Matrices for tissue engineering-scaffold structure for a bioartificial liver support system

Cited by 75 publications

References 23 publications

Hydrogel-coated textile scaffolds as candidate in liver tissue engineering: II. Evaluation of spheroid formation and viability of hepatocytes

Hydrogel-coated textile scaffolds as candidate in liver tissue engineering: II. Evaluation of spheroid formation and viability of hepatocytes

Proliferating Cells versus Differentiated Cells in Tissue Engineering

Preparation and cytocompatibility of silk fibroin/chitosan scaffolds

Contact Info

Product

Resources

About