Matrix effect evaluation and validation of the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation scavenging assay, as well as its application using a tejate, an ancient beverage in Mexico

Gonzalez-Rivera, Maria L.; Martinez-Morales, Flavio; Alonso-Castro, Ángel Josabad; López-Rodríguez, Juan Francisco; Aranda‐Romo, Saray; Zapata-Morales, Juan Ramón; Aragón-Martínez, Othoniel Hugo

doi:10.1007/s11696-019-00829-3

Cited by 5 publications

(6 citation statements)

References 48 publications

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“…The salivary thiol antioxidants, like glutathione and albumin, reduce the ABTS radical cation efficiently, whereas the same antioxidants do not reduce the Fe 3+-TPTZ efficiently to Fe 2+-TPTZ. 6,22 It is important to mention that one crucial advantage of a spectrophotometric measurement, like SAC, over the measure of an individual antioxidant compound using a liquid chromatographic or mass spectrometric technique is that it considers the additive, synergistic and antagonistic effects of antioxidant mixtures in actual samples. 22 ABTS scavenging finding agreed with a previous report that shows a reduced antioxidant balance (low glutathione peroxidase activity) in pregnant women, compared with nonpregnant women.…”

Section: Discussionmentioning

confidence: 99%

“…6,22 It is important to mention that one crucial advantage of a spectrophotometric measurement, like SAC, over the measure of an individual antioxidant compound using a liquid chromatographic or mass spectrometric technique is that it considers the additive, synergistic and antagonistic effects of antioxidant mixtures in actual samples. 22 ABTS scavenging finding agreed with a previous report that shows a reduced antioxidant balance (low glutathione peroxidase activity) in pregnant women, compared with nonpregnant women. 7 The proteins and PARS were not shown to have a role in the reduced ABTS-SAC in pregnant women (Figure 1 and Figure 2).…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, 200 µL of each mixture was placed in a well of a 96-well plate to read its absorbance at 596 nm. Meanwhile, the ABTS radical scavenging assay was performed as described by Gonzalez-Rivera et al, 22 Phosphotungstic acid reactive substances (PARS) in saliva PARS technique was based on a previous methodology. 23 In brief, 100 µL of the saliva supernatant was added to 200 µL of phosphotungstic acid reagent.…”

Section: Salivary Antioxidant Capacity (Sac)mentioning

confidence: 99%

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Correlation between salivary biomarkers and plaque-induced gingivitis exacerbated by pregnancy

Aragón-Martínez¹,

Loredo-Escobar²,

Bravo³

et al. 2022

J Oral Res

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Objetive: To evaluate the correlation between salivary biomarkers (the salivary antioxidant ability, salivary level of polyphenols, and other antioxidants) with plaque-induced gingivitis exacerbated by pregnancy in pregnant and nonpregnant women. Material and Methods: For this observational study, medical records, dental examinations, and analyses of saliva samples were carried out in pregnant and nonpregnant women. A p-value of <0.05 was considered significant. Results: The pregnant women (n =17) exhibited a lower antioxidant capacity (p-value=0.0041), higher levels of polyphenols, gingival index, bleeding on probing, and subjects consuming mineral-enriched products (p-value from <0.0001 to 0.0466), and unchanged levels of phosphotungstic acid reactive substances, proteins, oral hygienic habits, plaque index and probing depth (p-value from 0.0683 to 0.8358), in comparison with the nonpregnant women (n=9). Also, a positive correlation between the gingival index and salivary polyphenol content was observed (r-value = 0.4087, p-value = 0.0202). Conclusion: The salivary polyphenols correlate with plaque-induced gingivitis exacerbated by pregnancy, suggesting a deficiency of salivary antioxidant protection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Salivary Antioxidant Capacity (Sac)mentioning

confidence: 99%

See 1 more Smart Citation

Correlation between salivary biomarkers and plaque-induced gingivitis exacerbated by pregnancy

Aragón-Martínez¹,

Loredo-Escobar²,

Bravo³

et al. 2022

J Oral Res

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“…The antioxidant abilities of compounds can be tested with various assays in vitro that expose the compounds to different free synthetic radicals, such as the 2,2 -diphenyl-1-picrylhydrazyl (DPPH) or 2,2 -azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical. The scavenging activity of the antioxidant against the radical is monitored through the inherent spectral properties of the intact radical [9,10].…”

Section: Introductionmentioning

confidence: 99%

Dactylopius opuntiae [Cockerell] Could Be a Source of Antioxidants for the Preservation of Beef Patties

Aragon-Martinez,

Martinez-Morales,

González-Chávez

et al. 2023

Insects

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Dactylopius opuntiae is an insect pest that contains at least carminic acid, which has antioxidant properties. Since there is a relationship between the antioxidant ability and preservative action of compounds applied to meat products, the purpose of this study was to evaluate the antioxidant activity and usefulness of a D. opuntiae extract for beef patty preservation. The insects were bred and processed to obtain a liquid extract. For the extract, its carminic acid content, antioxidant activity against two free radicals, and actions on food quality parameters were determined. The D. opuntiae dry powder contained 2.91% w/w carminic acid, while the liquid extract exhibited an IC50 value of 3437.8 ± 67.8 and 19633.0 ± 674.5 µg/mL against the DPPH and ABTS radicals. Nevertheless, these antioxidant actions were lower than those found in a D. coccus extract. The D. opuntiae extract improved in a short time the redness and yellowness, eliminated the unfavorable effect of their vehicle on the MetMb level, and greatly reduced the TBARS formation. For the first time, an extract of D. opuntiae was applied to beef patties, and its beneficial antioxidant action on meat acceptance parameters was confirmed, which has potential commercial applications.

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“…All the validation and identification tests results were within the acceptability criteria stipulated by the FDA and International Conference on Harmonization (ICH) guidelines (ICH, 2005; US Department of Health and Human Services et al, 2018), except the stability results for the high QC samples stored at -18 ºC for 24 h. For this reason, the preparation of stock, standard solutions and experimental samples were on the same day they of the assays. For quantitative determinations, the application of the guidelines described by the FDA and ICH guarantees the reliability and strict control on the results, as well as the suitability, accuracy, and reproducibility of the assay (Gonzalez-Rivera et al, 2019).…”

mentioning

confidence: 99%

Evaluation of the antioxidant interaction between butylated hydroxytoluene and quercetin and their utility for beef patties preservation

Martinez-Morales¹,

Zapata-Morales²,

Aragón-Martínez³

2022

BIOTECNIA

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The present study aimed to find a mixture of BHT and quercetin with an antioxidant synergism that would enable both compounds to be used at low concentrations and, subsequently, evaluate the preservative action of the combination selected on ground beef patties stored at 4 ºC. Our results showed that quercetin possessed a higher antioxidant activity than BHT, and of the five combinations tested, a 1:5 BHT-quercetin combination presented a synergistic antioxidant activity. For the refrigerated beef patties, the 1:5 combination (5.2 and 26.0 mg/kg of BHT and quercetin, respectively) produced a beneficial action on the redness, yellowness, antioxidant capacity, metmyoglobin content, and thiobarbituric acid reactive substance value, which was similar to that produced by 100 or 36 mg/kg of BHT or quercetin (P >0.05, except the redness induced by quercetin). These benefits were compared with the data obtained from a preservative-free patty control sample (P <0.001 to <0.05). Our study shows, for the first time, that the use of a 1:5 BHT-quercetin combination was an effective, simple, and available method to preserve refrigerated beef patties, which can substitute the individual addition of high concentrations of these compounds by the meat industry.

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Matrix effect evaluation and validation of the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation scavenging assay, as well as its application using a tejate, an ancient beverage in Mexico

Cited by 5 publications

References 48 publications

Correlation between salivary biomarkers and plaque-induced gingivitis exacerbated by pregnancy

Correlation between salivary biomarkers and plaque-induced gingivitis exacerbated by pregnancy

Dactylopius opuntiae [Cockerell] Could Be a Source of Antioxidants for the Preservation of Beef Patties

Evaluation of the antioxidant interaction between butylated hydroxytoluene and quercetin and their utility for beef patties preservation

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