Matrix Effect in the Quantitative Determination of Mandelic and Phenylglyoxylic Acid in Urine Samples by HPLC-MS/MS with Isotopic Dilution

Paci, Enrico; Pigini, Daniela; Caporossi, Lidia; Rosa, Mario De; Santoro, A.; Sisto, Renata; Papaleo, Bruno; Tranfo, Giovanna

doi:10.2174/1573411011309030013

Cited by 13 publications

(1 citation statement)

References 12 publications

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“…All the metabolites have been determined by HPLC-MS/MS (Series 200 LC pump, PerkinElmer, Norwalk, CT, USA coupled with an API 4000 triple-quadrupole mass spectrometry detector, AB/Sciex, Ontario, Canada) equipped with a Turbo Ion Spray (TIS), in the urine samples of workers, both before and after the working shift. SPMA, cotinine, their deuterium-labeled internal standards and MA and PGA were determined following the method described in Tranfo et al (26), and in Paci et al (27), respectively. SBMA and MHIPPs were determined by suitably changing the method by Sabatini et al (28).…”

Section: Biological Monitoringmentioning

confidence: 99%

Direct and Oxidative DNA Damage in a Group of Painters Exposed to VOCs: Dose – Response Relationship

Sisto

Cavallo

Ursini

et al. 2020

Front. Public Health

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Volatile organic compounds (VOCs) are present in several working activities. This work is aimed at comparing oxidative stress and DNA damage biomarkers to specific VOCs in the occupational exposure of painters. Dose-response relationships between biomarkers of oxidative stress and of dose were studied. Unmetabolized VOCs and their urinary metabolites were analyzed. Urinary Methylhyppuric acids (MHIPPs, xylenes metabolite), Phenylglyoxylic and Mandelic acid (PGA, MA ethylbenzene metabolites), S-Benzylmercapturic acid (SBMA, toluene metabolite), and S-Phenylmercapturic acid (SPMA, benzene metabolite) were quantified at the end of work-shift. Oxidative stress was determined by: urinary excretion of 8-oxodGuo, 8-oxoGua and 8-oxoGuo and direct/oxidative DNA damage in blood by Fpg-Comet assay. Multivariate linear regression models were used to assess statistical significance of the association between dose and effect biomarkers. The regressions were studied with and without the effect of hOGG1 and XRCC1 gene polymorphisms. Statistically significant associations were found between MHIPPs and both 8-oxoGuo and oxidative DNA damage effect biomarkers measured with the Comet assay. Oxidative DNA damage results significantly associated with airborne xylenes and toluene, whilst 8-oxodGuo was significantly related to urinary xylenes and toluene. Direct DNA damage was significantly associated to SBMA. XRCC1 wild-type gene polymorphism was significantly associated with lower oxidative and total DNA damage with respect to heterozygous and mutant genotypes. The interpretation of the results requires some caution, as the different VOCs are all simultaneously present in the mixture and correlated among them.

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Section: Biological Monitoringmentioning

confidence: 99%

Direct and Oxidative DNA Damage in a Group of Painters Exposed to VOCs: Dose – Response Relationship

Sisto

Cavallo

Ursini

et al. 2020

Front. Public Health

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A review of extraction methods and analytical techniques for styrene and its metabolites in biological matrices

Rahimian

Soleimani

2022

Biomedical Chromatography

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We reviewed the toxicokinetics of styrene to introduce reliable surrogates for the biological monitoring of styrene workers. We have also discussed the extraction techniques and analytical methods of styrene and its metabolites. Sample preparation is the main bottleneck of the analytical techniques for styrene and its metabolites. Although some microextraction methods have been developed to overcome such disadvantages, some still have limitations such as long extraction time, fiber swelling and breakage, and the cost and the limited lifetime of the fiber. Among all, microextraction by packed sorbents, coupled with HPLC with ultraviolet detection (MEPS‐HPLC‐UV), can be the method of choice for determining styrene metabolites. Few studies investigated unchanged styrene in breath samples. Chemical determination of styrene in exhaled breath provides new insights into organ toxicity in workers with inhalation exposures and can be considered a fascinating tool in risk assessment strategies. Taking blood samples is invasive and less accepted by workers. In contrast, breath analysis is the most attractive method for workers because breath samples are easy to collect and non‐invasive, and sample collection does not require the transfer of workers to health facilities. Therefore, developing selective and sensitive methods for determining styrene in breath samples is recommended for future studies.

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Simultaneous determination of urinary 31 metabolites of VOCs, 8-hydroxy-2′-deoxyguanosine, and trans-3′-hydroxycotinine by UPLC-MS/MS: 13C- and 15N-labeled isotoped internal standards are more effective on reduction of matrix effect

Kuang

Jiang

et al. 2019

Anal Bioanal Chem

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Matrix Effect in the Quantitative Determination of Mandelic and Phenylglyoxylic Acid in Urine Samples by HPLC-MS/MS with Isotopic Dilution

Cited by 13 publications

References 12 publications

Direct and Oxidative DNA Damage in a Group of Painters Exposed to VOCs: Dose – Response Relationship

Direct and Oxidative DNA Damage in a Group of Painters Exposed to VOCs: Dose – Response Relationship

A review of extraction methods and analytical techniques for styrene and its metabolites in biological matrices

Simultaneous determination of urinary 31 metabolites of VOCs, 8-hydroxy-2′-deoxyguanosine, and trans-3′-hydroxycotinine by UPLC-MS/MS: 13C- and 15N-labeled isotoped internal standards are more effective on reduction of matrix effect

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