Matrix Effects in Liquid Chromatography–Electrospray Ionization–Mass Spectrometry

Stahnke, Helen; Alder, Lutz

doi:10.1002/9781119070771.ch7

Cited by 9 publications

(5 citation statements)

References 162 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 Statistical evaluation of matrix effects in DW and WWTP effluent after enrichment with the mlSPE method (left) and evaporation method (right). The dashed orange lines mark + 25% and − 25% hypothesis was confirmed by Ekdahl et al [21] and Stahnke et al [22] who observed suppression of the target analyte signals, simultaneously to an increasing TIC. A comparison of the TIC of the mlSPE-enriched WWTP effluent with the TIC of mlSPE-enriched drinking water clearly demonstrated that an increased TIC intensity coincided with more pronounced ion suppression (ESM Fig.…”

Section: Structure-unspecific Matrix Effectssupporting

confidence: 56%

Matrix effects in the analysis of polar organic water contaminants with HILIC-ESI-MS

et al. 2020

View full text Add to dashboard Cite

Matrix effects have been shown to be very pronounced and highly variable in the analysis of mobile chemicals, which may severely exacerbate accurate quantification. These matrix effects, however, are still scarcely studied in combination with hydrophilic interaction liquid chromatography (HILIC) and for very polar chemicals. In this study, the matrix effects of 26 polar model analytes were investigated in enriched drinking water, wastewater treatment plant effluent and solutions of inorganic salts, utilizing post-column infusion of the analytes into a HILIC-electrospray ionisation (ESI)-high-resolution mass spectrometry system. These experiments revealed the occurrence of structure-specific and unspecific matrix effects. The unspecific matrix effects were mainly observed in positive ESI polarity and predominantly coincided with a high ion count, resulting in ion suppression of all analytes. Thus, the excess charge is hypothesized to be the limiting factor in ion formation. Structurespecific matrix effects were more pronounced in negative ESI polarity and even structurally similar compounds were observed to react entirely differently: perfluoroalkyl carboxylic acids were suppressed, while perfluoroalkane sulfonic acids were simultaneously enhanced. These matrix effects were traced back to inorganic anions and cations, which eluted over a significant fraction of the chromatographic run time with this setup. Hence, it was concluded that inorganic ions are a main cause for matrix effects in the analysis of mobile chemicals utilizing HILIC.

show abstract

Section: Structure-unspecific Matrix Effectssupporting

confidence: 56%

Matrix effects in the analysis of polar organic water contaminants with HILIC-ESI-MS

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In various review articles, different approaches have been discussed to overcome this analytical problem [13,41,102,103]. As reported by Ucles et al [103], several approaches are used to eliminate or reduce matrix effects; they include optimizing sample preparation to remove interfering compounds from the samples [104], changing LC conditions to avoid the coelution of analytes and interfering compounds [105], changing MS conditions to reduce the occurrence of the matrix effect in the ion source [106], dilution of the sample [106] or using chemical treatment measures [107] or calibration techniques such as matrix-matched calibrations [29], and the standard addition method [13].…”

Section: Sample Determinationmentioning

confidence: 99%

LC-MS/MS Determination of Pesticide Residues in Fruits and Vegetables

Stachniuk¹

2018

Reference Series in Phytochemistry

View full text Add to dashboard Cite

A proper diet is commonly regarded as one of the most important factors determining one's health. A key role in such a diet is played by unprocessed food of plant origin, mainly fruits and vegetables, as they contain many important dietary bioactive compounds such as polyphenols, carotenoids, fiber, antioxidants, several important vitamins, and minerals. Despite the nutritional benefit, they may also contain substances that adversely affect human health. Pesticides constitute a special group of contaminants as even small amounts of these substances can result in acute poisoning, lead to cancer, and have an adverse impact on the endocrine, immune, and nervous system. A considerable number of pesticides have a harmful effect already in low concentrations, within the range of μg kg À1 and below μg kg À1 ; hence, there is a great need for identifying and determining them by means of highly selective and sensitive methods. In the analysis of pesticide residue, similarly to the analysis of other food contaminants,

show abstract

“…Low (− 20 % < ME < 20 %) or medium (− 50 % < ME < − 20 % or 20 % > ME > 50 %) matrix effects (ME) (Economou, Botitsi, Antoniou, & Tsipi, 2009) were observed for all analytes, demonstrating the great efficiency of d-SPE cleanup on matrix co-extractives removal (Table 4). Signal suppression was prevalent, such phenomenon has been supported by different theories as detailed by Stahnke and Alder (2015). In summary, co-eluted endogenous (matrix components) and exogenous (others introduced during sample preparation) substances can suppress the analytical signal by interfering with the addition of charge to the analyte in the liquid phase and/or transfer of ions from the droplet surface to the gas phase during the ionization process stages (Furey, Moriarty, Bane, Kinsella, & Lehane, 2013;Panuwet et al, 2016).…”

Section: In-house Validationmentioning

confidence: 91%

Bisphenol A and its structural analogues in infant formulas available in the Brazilian market: Optimisation of a UPLC-MS/MS method, occurrence, and dietary exposure assessment

Petrarca

Perez²,

Tfouni³

2022

Food Research International

View full text Add to dashboard Cite

Matrix Effects in Liquid Chromatography–Electrospray Ionization–Mass Spectrometry

Cited by 9 publications

References 162 publications

Matrix effects in the analysis of polar organic water contaminants with HILIC-ESI-MS

Matrix effects in the analysis of polar organic water contaminants with HILIC-ESI-MS

LC-MS/MS Determination of Pesticide Residues in Fruits and Vegetables

Bisphenol A and its structural analogues in infant formulas available in the Brazilian market: Optimisation of a UPLC-MS/MS method, occurrence, and dietary exposure assessment

Contact Info

Product

Resources

About