Matrix metalloproteinase‐8 levels and elastase activities in gingival crevicular fluid from chronic adult periodontitis patients

Abstract

Similarities between MMP-8 and elastase probably reflect the fact that both enzymes are associated mainly with neutrophils: MMP-8 levels may have fallen more after treatment because the assay, unlike that for elastase, would most likely not have detected much enzyme bound to alpha-macroglobulin. The immunoassay for MMP-8 is more specific and convenient than functional collagenase assays, and might be suitable for monitoring the periodontal condition.

The effects of the initial treatment phase and of adjunctive low-dose doxycycline therapy on clinical parameters and MMP-8, MMP-9, and TIMP-1 levels in the saliva and peripheral blood of patients with chronic periodontitis

“…[39], polymorphonuclear leukocytes are the main source of so-called salivary MMPs. MMPs derived from inflammatory infiltrate cells, but not stationary cells, are mainly responsible for the destruction of tooth-supporting tissues in the course of chronic periodontitis, previously known as adult periodontitis [10,36,40]. These MMPs include the neutrophil collagenase MMP-8 and gelatinase MMP-9.…”

Proanthocyanidins Rapidly Stabilize the Demineralized Dentin Layer

“…3A, 3B). Given the high concentration of collagenase with which we chose to challenge the samples, which was 2 to 6 orders of magnitude higher than the MMP-8 level found in human oral fluids (Liede et al, 1999;Chen et al, 2000;Mäntylä et al, 2003), it comes as no surprise that the untreated demineralized collagen disappeared completely after 1 hr of digestion (Figs. 1B, 3C).…”

Markers of inflammation in periodontal diseases

“…Chen et al [18] observed that a concentration of MMP-8 in gingival crevicular fluid is positively correlated with the plaque index and bleeding index, whereas the total amount of MMP-8 in GCF is also correlated with the depth of periodontal pockets in patients with periodontitis.…”