Matrix metalloproteinase-9 (MMP-9) as prospective histopathological and molecular biomarker for oral squamous cell carcinoma

Atla, Bhagyalakshmi; Prabhakula, Sreevalli; Kumar, Seeram Satish; Boni, Lakshmi Saraswathi

doi:10.18203/2320-6012.ijrms20183273

Cited by 6 publications

(6 citation statements)

References 9 publications

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“…Our study observed a statistically significant relationship while comparing the grades of staining intensity and MMP-9 expression with the development of oral squamous cell carcinoma (Table 3). This observation is consistent with other studies reported by Jose et al 25,26 . Our study showed that MMP-9 was not expressed in normal mucosa among healthy individuals.…”

Section: Discussionsupporting

confidence: 94%

Immunoexpression of Matrix Metalloproteinase-9 in Histopathological tissue samples of oral Squamous Cell Carcinoma

Shafiq¹,

Khan²,

Ahmad³

et al. 2021

PJMHS

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Background: Oral cancer represents a significant component of the global cancer burden. It develops as a result of multiple risk factors. The main histological form of oral cancer is oral squamous cell carcinoma (OSCC). The disintegration of the extracellular matrix (ECM) is one of the key hallmarks of cancer progression, which stimulates the invasion of tumoral cells into the neighbouring tissue. Matrix metalloproteinases-9 (MMP-9) can degrade different elements of the ECM and basement membrane. Methods: A total of 35 histologically diagnosed cases of OSCC (n=25) and healthy individuals (n=10) as control were included. Immunohistochemical staining was performed on all cases. The results were analysed using SPSS version 20. Results: Increased expression of MMP-9 was observed in OSCC cases (n=23/25; 92%) as compared to healthy individuals. Statistically, a significant relation was observed while comparing the grade of staining intensity and MMP-9 expression among OSCC cases (p=0.001). Conclusion: The present study is planned to assess the immunohistochemical expression of MMP-9 in diagnosed cases of OSCC and healthy individuals. Increased expression of MMP-9 was observed in OSCC as compared to healthy individuals. Evaluation of MMP-9 could be of clinical importance in OSCC and can be used as a diagnostic predictive marker of OSCC. Keywords: Oral squamous cell carcinoma; Matrix Metalloproteinase -9; Immunohistochemistry

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Section: Discussionsupporting

confidence: 94%

Immunoexpression of Matrix Metalloproteinase-9 in Histopathological tissue samples of oral Squamous Cell Carcinoma

Shafiq¹,

Khan²,

Ahmad³

et al. 2021

PJMHS

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“…This established a statistically significant association between a high expression of the marker and the advanced stage of the tumor (p<0.001). These findings are in accordance with studies conducted by Atla B et al [ 14 ] and Sowmya V et al [ 15 ], which showed a significant association between the expression of the protein and an advanced stage of the tumor.…”

Section: Discussionsupporting

confidence: 93%

“…In the study conducted by Atla B et al [ 14 ], 70% of the cases with lymph node metastases had a high expression of the marker, and the remaining 30% had a low expression. Among the cases without lymph node metastases, 82.2% had a low expression, and 17.8% had a high expression of the marker.…”

Section: Discussionmentioning

confidence: 99%

Role of Matrix Metalloproteinase 9 in Predicting Lymph Node Metastases in Oral Squamous Cell Carcinoma

Chakraborty¹,

Suresh²,

Mohiyuddin³

2023

Cureus

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BackgroundOral cancer is a common malignancy worldwide, with approximately 3,50,000 new cases diagnosed yearly. Out of many factors which affect the survival in patients with oral cancer, lymph node metastases are a major factor that reduces survival by 50%. Even though many biomarkers have been studied to predict lymph node metastasis, none have yet been accepted for routine use. Matrix metalloproteinases (MMPs) play a vital role in extracellular matrix (ECM) degradation, thus facilitating the invasive potential and the metastatic cascade of tumors. Of the different subtypes, multiple studies have demonstrated that matrix metalloproteinase 9 (MMP9) overexpression is often associated with the aggressive nature of the tumor. Therefore, this investigation is done to know the role of MMP9 in predicting lymph node metastasis in oral squamous cell carcinoma (OSCC). AimTo determine the immunohistochemical expression of MMP9 in OSCC and to find its association with lymph node metastasis. Settings and designIt is a laboratory-based observational study. Materials and methodsOne hundred five histologically proven cases of OSCC were studied. Histopathological parameters like depth of invasion, presence of lymph node metastasis, grading, and TNM staging were done according to the 8th AJCC staging criteria. Both intensity and proportion of MMP9 expression were recorded. Statistical analysisFor qualitative data, the Chi-square test was used as a test of significance. The p-value (probability that the result is true) of <0.05 was considered statistically significant after assuming all the rules of statistical tests. ResultsA higher expression of MMP9 was observed in 56.2% of cases and the higher expression correlated with the presence of lymph node metastases (p<0.001), an advanced stage of cancer (P <0.001), and grade of the tumor (p=0.003). ConclusionA positive association between MMP9 and lymph node metastasis and pathological TNM staging demonstrates MMP9 as a potential biomarker to predict the behavior of the tumor.

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“…Ikebe et al 64 pointed out that MMP-9 overexpression could lead to invasion but not metastasis in oral carcinogenesis. Atla et al 56 reported over expression of MMP-9 in stage III oral tumors. Champatyray et al 65 reported immunoexpression pattern of MMP-9 in different grades of oral squamous cell carcinoma.…”

Section: Resultsmentioning

confidence: 99%

“…54 The expression of VEGF in tumor tissues is directly proportional to the invasion depth of carcinogenesis. 55 Atla et al 56 focused on MMP-9 as an important molecular and histopathological marker of carcinoma and thus could be used as a therapeutic target in oral cancer. A positive correlation between MMP-2 and 9 expression and survival outcome has been reported in several types of cancers.…”

Section: Resultsmentioning

confidence: 99%

Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis

2020

TJNPR

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Abnormal cell proliferation, invasion, metastasis and angiogenesis are the most prominent features of malignant tumours. The present study evaluated the modulating effect of myrtenal on the immunoexpression pattern of cell proliferative (PCNA and cyclin D1), angiogenic (VEGF) and invasive (MMP-2 and MMP-9) markers in 7,12-dimethylbenz(a)anthracene (DMBA)induced experimental oral carcinogenesis in golden Syrian hamsters using immunohistochemical assay. Topical application (painting) of 0.5% DMBA (six hamsters), a site specific carcinogen, three times a week for 14 weeks resulted in the formation of tumors in the buccal pouches of golden Syrian hamsters , which was confirmed by histopathological studies. Buccal mucosa excised from the hamsters treated with DMBA alone (tumour-bearing hamsters) showed abnormal immunoexpression pattern of cell proliferative, angiogenic and invasive markers. Myrtenal administration (230 mg/kg b.w) orally to DMBA treated hamsters significantly downregulated the expression of the above said molecular markers in the chemopreventive phase and considerably decreased the expression pattern in the chemotherapeutic phase. The findings from this study, thus support the anti-cell proliferative, anti-angiogenic, and antiinvasive potential of myrtenal in DMBA-induced hamster buccal pouch carcinoma.

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Matrix metalloproteinase-9 (MMP-9) as prospective histopathological and molecular biomarker for oral squamous cell carcinoma

Cited by 6 publications

References 9 publications

Immunoexpression of Matrix Metalloproteinase-9 in Histopathological tissue samples of oral Squamous Cell Carcinoma

Immunoexpression of Matrix Metalloproteinase-9 in Histopathological tissue samples of oral Squamous Cell Carcinoma

Role of Matrix Metalloproteinase 9 in Predicting Lymph Node Metastases in Oral Squamous Cell Carcinoma

Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis

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