Matrix Metalloproteinases and Their Tissue Inhibitors in the Developing Neonatal Mouse Uterus1

Hu, Jianbo; Zhang, Xuan; Nothnick, Warren B.; Spencer, Thomas E.

doi:10.1095/biolreprod.104.031559

Cited by 35 publications

(31 citation statements)

References 37 publications

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“…Both MMP2 and MMP9 were detected in the murine uterus (Hu et al 2004) and human endometrial cells (Martelli et al 1993, Iwahashi et al 1996. In the prepubertal porcine uterus, RLX increased the secretion of both MMP2 and MMP9 (Lenhart et al 2001).…”

Section: Discussionmentioning

confidence: 96%

Milk-borne lactocrine-acting factors affect gene expression patterns in the developing neonatal porcine uterus

Chen¹,

Frankshun²,

Wiley³

et al. 2011

Reproduction

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Lactocrine communication of milk-borne bioactive factors (MbFs) from mother to offspring through nursing can affect neonatal development with lasting consequences. Relaxin (RLX), a lactocrine-active peptide found in porcine colostrum, stimulates estrogen receptor-a (ESR1) expression required for uterine development shortly after birth (postnatal dayZPND 0). Whether other MbFs or cooperative lactocrine mechanisms affect the neonatal uterine developmental program is unknown. To determine the effects of age, nursing, and exogenous RLX on gene expression associated with uterine development, gilts (nZ4-5/group) were assigned to nurse ad libitum or to receive milk replacer, with or without exogenous RLX (20 mg/kg BW i.m./6 h for 48 h), from birth to PND 2 when uteri were collected. Body weight and uterine weight increased (P!0.05) similarly from birth to PND 2 in all gilts. However, colostrum consumption was required for normal uterine ESR1, vascular endothelial growth factor (VEGFA), matrix metalloproteinase 9 (MMP9), and RLX receptor (RXFP1) protein and/or transcript expression on PND 2. Uterine ESR1, VEGFA, and MMP9 protein levels were below (P!0.01) the assay sensitivity in replacer-fed gilts. Supplemental RLX increased (P!0.05) uterine ESR1 protein and mRNA in nursed gilts, as well as VEGFA protein in nursed and VEGFA mRNA in both nursed and replacer-fed gilts. RLX treatment did not affect uterine MMP9 mRNA levels. When compared with replacer-fed gilts on PND 2, uterine RXFP1 mRNA was reduced (P!0.05) in nursed gilts and in RLX-supplemented replacer-fed gilts. These results constitute the first evidence that establishment of the neonatal porcine uterine developmental program requires maternal lactocrine support.

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Section: Discussionmentioning

confidence: 96%

Milk-borne lactocrine-acting factors affect gene expression patterns in the developing neonatal porcine uterus

Chen¹,

Frankshun²,

Wiley³

et al. 2011

Reproduction

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“…ECM remodeling has been analyzed in human pathological pregnancy (4), in the murine developing neonatal uterus (42), and in ruminants (75,87). Matrix metalloproteinases (MMP) induce ECM degradation and are regulated by tissue inhibitors of MMP (TIMP) that could also act as proliferation and differentiation factors in a paracrine/autocrine mechanism (15).…”

Section: Cell Adhesion and Endometrial Remodeling At Implantationmentioning

confidence: 99%

Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation

Mansouri-Attia

Aubert

Reinaud

et al. 2009

Physiological Genomics

153

148

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At implantation the endometrium undergoes modifications necessary for its physical interactions with the trophoblast as well as the development of the conceptus. We aim to identify endometrial factors and pathways essential for a successful implantation in the caruncular (C) and the intercaruncular (IC) areas in cattle. Using a 13,257-element bovine oligonucleotide array, we established expression profiles at day 20 of the estrous cycle or pregnancy (implantation), revealing 446 and 1,295 differentially expressed genes (DEG) in C and IC areas, respectively (false discovery rate ϭ 0.08). The impact of the conceptus was higher on the immune response function in C but more prominent on the regulation of metabolism function in IC. The C vs. IC direct comparison revealed 1,177 and 453 DEG in cyclic and pregnant animals respectively (false discovery rate ϭ 0.05), with a major impact of the conceptus on metabolism and cell adhesion. We selected 15 genes including C11ORF34, CXCL12, CXCR4, PLAC8, SCARA5, and NPY and confirmed their differential expression by quantitative RT-PCR. The cellular localization was analyzed by in situ hybridization and, upon pregnancy, showed gene-specific patterns of cell distribution, including a high level of expression in the luminal epithelium for C11ORF34 and MX1. Using primary cultures of bovine endometrial cells, we identified PTN, PLAC8, and CXCL12 as interferon-(IFNT) target genes and MSX1 and CXCR7 as IFNT-regulated genes, whereas C11ORF34 was not an IFNT-regulated gene. Our transcriptomic data provide novel molecular insights accounting for the biological functions related to the C or IC endometrial areas and may contribute to the identification of potential biomarkers for normal and perturbed early pregnancy.transcriptome; interferon-tau; pregnancy; cattle IN MAMMALS, the establishment and maintenance of pregnancy require a subtle and tightly regulated communication between the conceptus (embryo and embryonic annexes) and the maternal environment (85). The success of implantation relies on several essential steps including the adjustment of the uterine environment to support the development of the conceptus and the profound remodeling of the endometrium structure necessary for the apposition, adhesion, and invasion phases (36). In contrast to human and rodents, the invasion of the maternal tissue by the fetal tissue is very limited in ruminants (71) and leads to a synepitheliochorial placentation (86). Since the trophoblast appears to be intrinsically invasive in mammals (11), apposition, adhesion, and invasion processes are thought to be controlled by the endometrium (83). In mammalian species presenting an invasive implantation, decidua restrains the invasion of the embryo in a spatiotemporal manner (20). The expression and the regulation of some factors involved in the apposition, adhesion, and invasion aspects of implantation have been reported in ruminants (62, 79), but, overall, the comparative cascade of molecular mechanisms remains largely unknown.The sequence of events occ...

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“…To protect the integrity of the extracellular matrix degrading enzymes, protein extraction was performed using detergents instead of heat as described by Hu et al [41]. Supernatants were then collected, and an aliquot was subjected to the DC Protein Assay (Bio-Rad Laboratories, Richmond, CA).…”

Section: Timp1 Protein Inhibitory Activitymentioning

confidence: 99%

Proteasomes and Proteasome Activator 200 kDa (PA200) Accumulate on Chromatin in Response to Ionizing Radiation

Blickwedehl¹,

McEvoy²,

Wong³

et al. 2007

Radiation Research

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Matrix Metalloproteinases and Their Tissue Inhibitors in the Developing Neonatal Mouse Uterus1

Cited by 35 publications

References 37 publications

Milk-borne lactocrine-acting factors affect gene expression patterns in the developing neonatal porcine uterus

Milk-borne lactocrine-acting factors affect gene expression patterns in the developing neonatal porcine uterus

Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation

Proteasomes and Proteasome Activator 200 kDa (PA200) Accumulate on Chromatin in Response to Ionizing Radiation

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