Matrix metalloproteinases as biomarkers of disease: updates and new insights

Galliera, Emanuela; Tacchini, Lorenza; Romanelli, Massimiliano Marco Corsi

doi:10.1515/cclm-2014-0520

Cited by 64 publications

(71 citation statements)

References 14 publications

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“…MMP family proteolytic enzymes are indispensable for the remodeling of extracellular matrix, of which the degradation is a prerequisite for the invasion and metastasis of cancer (27,28). In the present study, it was demonstrated that the protein expression level of MMP2 was notably decreased following miR-766-5p inhibitor treatment compared to that following treatment with the NC.…”

Section: Discussionsupporting

confidence: 45%

The role of miR-766-5p in cell migration and invasion in colorectal cancer

2018

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Abstract. Colorectal cancer (CRC) develops from the colon or rectum and is the fourth highest inducer of cancer mortality. In the present study, cancer tissues and normal tissues were extracted from patients with CRC who were treated in the Affiliated Hospital of Shandong University of Traditional Chinese Medicine (Jinan, China). Reverse transcription-quantitative polymerase chain reaction demonstrated that the expression level of miR-766-5p was significantly higher (P<0.01) in cancer tissue than that in normal tissue. SW480 cells were used for in vitro study and randomly separated into the miR-negative control (NC) inhibitor treatment group and miR-766-5p inhibitor treatment group. SW480 cell behaviors were evaluated. Results demonstrated that in the miR-766-5p inhibitor group, there was a decreased level of cell proliferation/migration/invasion and higher cell apoptosis compared with that in the miR-NC inhibitor group. miR-766-5p was predicted and verified to target the 3' untranslated region of suppressor of cancer cell invasion (SCAI) in SW480 cells. Protein expression levels of matrix metalloproteinase-2/phosphoinositide 3-kinase/AKT were decreased and SCAI was increased following miR-766-5p inhibitor treatment. In conclusion, the present study indicated that miR-766-5p inhibitor repressed the process of CRC by targeting SCAI.

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Section: Discussionsupporting

confidence: 45%

The role of miR-766-5p in cell migration and invasion in colorectal cancer

2018

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“…MMP-2 and MMP-9 are tightly regulated at the levels of transcription and secretion by multiple factors. For example, various stimulating factors can trigger MAPK, NF-jB and PYC signaling pathways, leading to effects on the GC box in the MMP-2 promoter region or to effects on AP-1 and NF-jB binding to the MMP-9 promoter region for expression regulation (Gallera et al, 2015). Therefore, in this study, we further speculated that curcumin likely down-regulated the transcription and inhibited the secretion of MMP-2 and MMP-9 by triggering MAPK and NF-jB, which ultimately caused partial suppression of SHI-1 cell migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

Curcumin induces apoptosis and suppresses invasion through MAPK and MMP signaling in human monocytic leukemia SHI-1 cells

Zeng

Dai

Jiang

et al. 2015

Pharmaceutical Biology

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Curcumin inhibited SHI-1 cell proliferation by arresting the cells in the S-phase, increasing the number of Annexin V-FITC + /PI À cells and promoting the loss of 4W m . The results of PCR and Western blotting showed that curcumin increased the FasL mRNA level; inhibited Bcl-2, NF-jB, and ERK expression; and activated P38 MAPK, JNK, and caspase-3. Additionally, curcumin partially suppressed SHI-1 cell invasion and attenuated the mRNA transcription and secretion of MMP-2 and MMP-9. Discussion and conclusion: This study demonstrates that curcumin not only induces SHI-1 cell apoptosis, possibly via both intrinsic and extrinsic pathways triggered by JNK, P38 MAPK and ERK signaling, but also partially suppresses SHI-1 cell invasion, likely by reducing the levels of transcription and secretion of MMP-2 and MMP-9. KeywordsAntitumor, matrix metalloproteinase, mitogen-activated protein kinase History

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“…[5]. They are important in creating an environment that support the initiation and maintenance of growth of primary and metastatic tumors, and have an important regulatory role, as they can modulate cytokines and chemokines activity by proteolytic processing [6].…”

Section: Introductionmentioning

confidence: 99%

Substrate-zymography: a still worthwhile method for gelatinases analysis in biological samples

Ricci

D’Esposito²,

Oriente³

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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Matrix metallo-proteinases (MMPs) are a family of zinc-dependent endopeptidases, capable of degrading all the molecular components of extracellular matrix. A class of MMPs is gelatinases which includes gelatinase A or MMP-2 (72 kDa) and gelatinase B or MMP-9 (92 kDa), which have been shown to play critical roles in pathophysiology of many human disease and, in particular, cancer progression. For these reasons they obtained a great interest as potential non-invasive biomarker in providing useful clinical information in cancer diagnosis and therapy. A sensitive and unexpensive method for analysis of gelatinases is the gelatine zymography, which allows to measure the relative amounts of active and inactive enzymes in body fluids and tissue extracts. The procedure involves the electrophoretic separation of proteins under denaturing but non reducing conditions through a polyacrylamide gel containing a synthetic substrate (gelatin). The aim of this mini-review has been to describe the general principles of gelatine zymography technique, underling the main advantages and disadvantages. Even though an improvement of this method is necessary for a better applicability in laboratory medicine, gelatine zymography represents the most convenient method to detect the activity of the different gelatinases from a wide range of biological samples.

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Matrix metalloproteinases as biomarkers of disease: updates and new insights

Cited by 64 publications

References 14 publications

The role of miR-766-5p in cell migration and invasion in colorectal cancer

The role of miR-766-5p in cell migration and invasion in colorectal cancer

Curcumin induces apoptosis and suppresses invasion through MAPK and MMP signaling in human monocytic leukemia SHI-1 cells

Substrate-zymography: a still worthwhile method for gelatinases analysis in biological samples

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