Matrix metalloproteinases (MMP-2 and MMP-9) activity in corneal ulcer and ocular surface disorders determined by gelatin zymography

Singh, Arti; Maurya, O. P. S.; Jagannadhan, M.V.; Patel, Ashok Kumar

doi:10.1007/s12177-012-9096-8

Cited by 16 publications

(15 citation statements)

References 14 publications

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“…Gelatin zymography is a method used for easy detection of latent and active MMPs. Because the active forms of MMPs are water-soluble enzymes, it is difficult to detect by immunohistochemical or molecular biological method ( 68 ).…”

Section: Discussionmentioning

confidence: 99%

“…Singh et al ( 68 ) demonstrated that the active forms of MMP-9 are present in tears of severe ulcerative and ocular surface disorder patients with no detection in the control group. Also, an injury without bacterial infection resulted in an increase in both MMP-2 and−9 and a slight but significant downregulation of TIMP-1 in mouse corneal ulcer ( 19 ).…”

Section: Discussionmentioning

confidence: 99%

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Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

et al. 2021

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Background: Corneal ulcer could be a major source of distress in small animals, with many contributing agents. In recent years, few studies evaluated the efficacy of platelet-rich plasma (PRP) in healing corneal ulcers.Aim: This study aimed to assess the ability of subconjunctival injection of autologous PRP in the treatment of corneal ulcers in dogs and cats as well as estimate the expression of matrix metalloproteinase (MMP)-2, MMP-9, and oxidative stress biomarkers in these patients.Methods: A total number of 28 animals (16 cats and 12 dogs) were enrolled in this study. Each animal was subjected to clinical, neurologic, and ophthalmic examinations where the type of ulcer was documented. Tear samples were collected for evaluation of oxidative biomarkers and MMPs; conjunctival swabs were taken to identify the involved organism. PRP was prepared from each animal and given as subconjunctival injection; numbers of injections were done according to case response. Clinical follow-up was done and documented for each case.Results: In cat patients, female and Persian cats were most affected; unilateral and superficial ulcers were most recorded. In male dogs, unilateral, and superficial ulcers were most recorded. FHV-1 was most identified in cats, while Staphylococcus aureus was most identified in dogs. Numbers of injections needed to achieve healing were recorded, with 50% of dogs needing two injections with 1-week intervals and 50% of cats needed three injections with 1-week intervals. Alterations in both oxidative biomarkers and MMPs were recorded in affected animals.Conclusion: The use of autologous PRP as a subconjunctival injection in treating corneal ulcers in dogs and cats is effective. The number of injections is the case and corneal ulcer type-dependent.Clinical Significance: Autologous PRP as a subconjunctival injection in treating corneal ulcer is a relatively cheap, safe method and can be done in the clinical setting.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

et al. 2021

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“…67 The role of MMPs in corneal melt, intuitive if one considers the predominance of collagen in the structure of corneal stroma, is well documented. 7,8,57,63,76,87 Specifically for NICM, the study of three cases of diclofenac-induced corneal melt with perforation revealed enhanced expression of MMPs 1 and 9 within corneal epithelial cells, stromal keratocytes, and at the level of the Descemet membrane. 34 Their uniform distribution pattern of expression was not consistent with the localization expected of a repair response.…”

Section: Matrix Metalloproteinasesmentioning

confidence: 99%

NSAID-induced corneal melt: Clinical importance, pathogenesis, and risk mitigation

Rigas

Huang

Honkanen

2020

Survey of Ophthalmology

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“…In the end, destining solution (10% acetic acid and 30% methanol) have been used in removing color. After gel drying, it was analyzed by Image J …”

Section: Methodsmentioning

confidence: 99%

Diallyl trisulfide inhibits cell migration and invasion of human melanoma a375 cells via inhibiting integrin/facal adhesion kinase pathway

Wang

Chu

Hsieh

et al. 2017

Environmental Toxicology

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Melanoma is the leading cause of death from skin disease due to its propensity for metastasis. Studies have shown that integrin-mediated focal adhesion kinase (FAK) signal pathway is implicated in cell proliferation, survival and metastasis of tumor cells. Our previous results indicated that diallyl trisulfide (DATS) provided its antimelanoma activity via inducing cell cycle arrest and apoptosis. The aim of this study was to explore DATS mediated antimetastatic effect and the corresponding mechanism in human melanoma A375 cells. We found that DATS exhibited an inhibitory effect on the abilities of migration and invasion in A375 cells under noncytotoxic concentrations analyzed by wound healing assays and Matrigel invasion chamber system. DATS attenuated invasion of A375 cells with characteristic of decreased activities and protein expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9. Moreover, DATS exerted an inhibitory effect on cell adhesion of A375 cells, which is in correlation with the change in integrin signaling pathway. Results of Western blotting showed that DATS decreased the levels of several integrin subunits, including α4, α5, αv, β1, β3 and β4. Subsequently, DATS induced a strong decrease in total FAK, phosphorylated FAK Tyr-397,-576, -577, and disorganized F-actin stress fibers, resulting in a nonmigratory phenotype. These results suggest that the antimetastatic potential of DATS for human melanoma cells might be due to the disruption of integrin/FAK signaling pathway.

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Matrix metalloproteinases (MMP-2 and MMP-9) activity in corneal ulcer and ocular surface disorders determined by gelatin zymography

Cited by 16 publications

References 14 publications

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

NSAID-induced corneal melt: Clinical importance, pathogenesis, and risk mitigation

Diallyl trisulfide inhibits cell migration and invasion of human melanoma a375 cells via inhibiting integrin/facal adhesion kinase pathway

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