Matrix Redox Physiology Governs the Regulation of Plant Mitochondrial Metabolism through Posttranslational Protein Modifications

Møller, Ian Max; Igamberdiev, Abir U.; Bykova, Natalia V.; Finkemeier, Iris; Rasmusson, Allan G.; Schwarzländer, Markus

doi:10.1105/tpc.19.00535

Cited by 78 publications

(73 citation statements)

References 176 publications

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“…Ascorbate is very important in mitochondrial metabolism where it participates in the ascorbate-glutathione cycle that removes H 2 O 2 produced by the respiratory chain e.g., [ 37 ]. The last step in ascorbate biosynthesis takes place on the outer surface of the IMM [ 38 ], but to date no ascorbate transporter has been identified in the IMM.…”

Section: The Different Transporter Classes and Familiesmentioning

confidence: 99%

“…In shotgun proteomics, many mitochondrial transporters have been observed to have PTMs of various types: (i) oxidations to give carbonylated side chains on primarily Lys and Pro or sulfoxide on Met [ 18 ], although the latter is probably an analytical artefact in some cases; (ii) phosphorylation of Ser, Thr, and Tyr [ 50 , 51 , 52 ]; (iii) acetylation of Lys [ 53 ]; and (iv) conjugation of Lys side chains with oxidative degradation products of polyunsaturated fatty acids, for instance, 4-hydroxynonenal (HNE) [ 54 , 55 ]. Many of these modifications are no doubt regulatory, while others are damaging and lead to proteolytic degradation [ 37 , 56 , 57 ].…”

Section: Posttranslational Modifications Of Transportersmentioning

confidence: 99%

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Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

et al. 2020

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To function as a metabolic hub, plant mitochondria have to exchange a wide variety of metabolic intermediates as well as inorganic ions with the cytosol. As identified by proteomic profiling or as predicted by MU-LOC, a newly developed bioinformatics tool, Arabidopsis thaliana mitochondria contain 128 or 143 different transporters, respectively. The largest group is the mitochondrial carrier family, which consists of symporters and antiporters catalyzing secondary active transport of organic acids, amino acids, and nucleotides across the inner mitochondrial membrane. An impressive 97% (58 out of 60) of all the known mitochondrial carrier family members in Arabidopsis have been experimentally identified in isolated mitochondria. In addition to many other secondary transporters, Arabidopsis mitochondria contain the ATP synthase transporters, the mitochondria protein translocase complexes (responsible for protein uptake across the outer and inner membrane), ATP-binding cassette (ABC) transporters, and a number of transporters and channels responsible for allowing water and inorganic ions to move across the inner membrane driven by their transmembrane electrochemical gradient. A few mitochondrial transporters are tissue-specific, development-specific, or stress-response specific, but this is a relatively unexplored area in proteomics that merits much more attention.

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Section: The Different Transporter Classes and Familiesmentioning

confidence: 99%

Section: Posttranslational Modifications Of Transportersmentioning

confidence: 99%

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

et al. 2020

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“…It is becoming widely acknowledged that many PTMs detected using advanced and highly sensitive MS techniques constitute biochemical noise of the system, not regulatory mechanisms [47,48]. Separating bona fide regulatory sites from biochemical noise now presents a major technological hurdle.…”

Section: Discussionmentioning

confidence: 99%

Rubisco lysine acetylation occurs at very low stoichiometry in mature Arabidopsis leaves: implications for regulation of enzyme function

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Scafaro

Fenske

et al. 2020

Biochemical Journal

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Multiple studies have shown ribulose-1,5-bisphosphate carboxylase/oxygenase (E.C. 4.1.1.39; Rubisco) to be subject to Lys-acetylation at various residues; however, opposing reports exist about the biological significance of these post-translational modifications. One aspect of the Lys-acetylation that has not been addressed in plants generally, or with Rubisco specifically, is the stoichiometry at which these Lys-acetylation events occur. As a method to ascertain which Lys-acetylation sites on Arabidopsis Rubisco might be of regulatory importance to its catalytic function in the Calvin–Benson cycle, we purified Rubisco from leaves in both the day and night-time and performed independent mass-spectrometry based methods to determine the stoichiometry of Rubisco Lys-acetylation events. The results indicate that Rubisco is acetylated at most Lys residues, but each acetylation event occurs at very low stoichiometry. Furthermore, in vitro treatments that increased the extent of Lys-acetylation on purified Rubisco had no effect on Rubisco maximal activity. Therefore, we are unable to confirm that Lys-acetylation at low stoichiometries can be a regulatory mechanism controlling Rubisco maximal activity. The results highlight the need for further use of stoichiometry measurements when determining the biological significance of reversible PTMs like acetylation.

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“…Another important mechanism affecting S-nitrosation state of proteins may be through TRX activity, which similarly to GSNOR, can degrade S-nitrosothiols to increase their turnover ratio ( Benhar et al, 2008 ). Several S-nitrosated enzymes that are summarized in this review have been also shown to be targets of mitochondrial TRX (see review in Møller et al, 2020 ). From plant S-nitrosation studies under abiotic stresses ( Fares et al, 2011 ; Camejo et al, 2013 ; Puyaubert et al, 2014 ), it could be suggested that under those conditions, the effect of the stress does not produce large changes on the S-nitrosation status of the cells, probably due to other mechanisms that could be competing with the S-nitrosation process.…”

Section: Mitochondrial Targets Of S-oxidation S-glutathionylation Smentioning

confidence: 99%

Thioredoxin Network in Plant Mitochondria: Cysteine S-Posttranslational Modifications and Stress Conditions

2020

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Plants are sessile organisms presenting different adaptation mechanisms that allow their survival under adverse situations. Among them, reactive oxygen and nitrogen species (ROS, RNS) and H 2 S are emerging as components not only of cell development and differentiation but of signaling pathways involved in the response to both biotic and abiotic attacks. The study of the posttranslational modifications (PTMs) of proteins produced by those signaling molecules is revealing a modulation on specific targets that are involved in many metabolic pathways in the different cell compartments. These modifications are able to translate the imbalance of the redox state caused by exposure to the stress situation in a cascade of responses that finally allow the plant to cope with the adverse condition. In this review we give a generalized vision of the production of ROS, RNS, and H 2 S in plant mitochondria. We focus on how the principal mitochondrial processes mainly the electron transport chain, the tricarboxylic acid cycle and photorespiration are affected by PTMs on cysteine residues that are produced by the previously mentioned signaling molecules in the respiratory organelle. These PTMs include S-oxidation, S-glutathionylation, Snitrosation, and persulfidation under normal and stress conditions. We pay special attention to the mitochondrial Thioredoxin/Peroxiredoxin system in terms of its oxidation-reduction posttranslational targets and its response to environmental stress.

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Matrix Redox Physiology Governs the Regulation of Plant Mitochondrial Metabolism through Posttranslational Protein Modifications

Cited by 78 publications

References 176 publications

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

Rubisco lysine acetylation occurs at very low stoichiometry in mature Arabidopsis leaves: implications for regulation of enzyme function

Thioredoxin Network in Plant Mitochondria: Cysteine S-Posttranslational Modifications and Stress Conditions

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