Maturation Events Leading to Transfer RNA and Ribosomal RNA

Mazzara, Gail P.; Plunkett, Guy; McClain, William H.

doi:10.1016/b978-0-12-289503-6.50016-3

Cited by 8 publications

(7 citation statements)

References 312 publications

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“…RNA processing involves a set of reactions that happen post-transcriptionally and prepares a RNA molecule for its function. The involvement of the three endonucleolytic RNA processing enzymes RNase III, RNase E, and RNase P has been well documented in the processing of Escherichia coli rRNA and tRNA (1)(2)(3)(4)(5)(6).…”

mentioning

confidence: 99%

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

Gurevitz¹,

Jain²,

Apirion³

1983

Proc. Natl. Acad. Sci. U.S.A.

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A precursor molecule for lOSb (MI) RNA, the RNA moiety of the RNA processing enzyme ribonuclease P (EC 3.1.26.5), is accumulated transiently in an Escherichia coli strain containing a plasmid that carries the lOSb RNA gene. The same RNA precursor molecule is accumulated, in relatively large quantities, in a temperature-sensitive RNase E-mutant at the nonpermissive temperature. The RNA precursor includes lOSb RNA and an extra 3' fragment that contains a termination stem and loop. It can be processed in vitro to a molecule the size of lOSb RNA. None of the four endoribonucleases of E. coli-RNase III, RNase E, RNase F, or RNase P-takes part in this cleavage reaction. Therefore, we suggest that the processing of the precursor-IOSb RNA to iOSb RNA is carried out by a thus-far unidentified endoribonuclease. The accumulation of a RNA molecule in a RNase E-mutant that does not contain a cleavage site for RNase E has been encountered previously and can be explained by assuming the existence of a RNA processing complex in the E. coli cell.Processing of RNA is an important aspect of RNA metabolism that plays a key role in the determination of the final population of RNA molecules in the cell. RNA processing involves a set of reactions that happen post-transcriptionally and prepares a RNA molecule for its function. The involvement of the three endonucleolytic RNA processing enzymes RNase III, RNase E, and RNase P has been well documented in the processing of Escherichia coli rRNA and tRNA (1-6).The processing enzyme RNase P is unique among all RNA processing enzymes because it consists of a protein and a RNA moiety (7-12). The RNA, MI, is required for RNase P activity (9) and is identical to the 10Sb RNA that accumulates in a strain containing the plasmid pL2 (pLN2) that partially complements the rnpA49 mutation (13,14). Introduction of the pL2 plasmid to E. coli strains leads to the accumulation of 1OSb (Ml) RNA, which is not a primary transcript (12)(13)(14).In the studies reported here we show that 10Sb (Ml) RNA, the RNA of RNase P, is derived from a precursor molecule (plOSb) that contains extra sequences at the 3' end, and we have established conditions for the in vitro processing of this precursor RNA. These experiments helped to identify a previously unrecognized RNA processing activity in E. coli. This precursor accumulates in a strain defective in the RNA processing enzyme RNase E, even though the enzyme RNase E does not process this RNA molecule. Therefore, the finding that this precursor accumulates in a RNase E-strain strengthens the concept of a complex of RNA processing enzymes in the E. coli cell.MATERIALS AND METHODS Strains. The rnp+ strain used was PP113 (15) and the rnpA49 strain was N2020 (16). The rne strain was N3441, which was derived from strain N3431 (17). To these strains the plasmid pL2 was introduced. This plasmid contains E. coli DNA that carries the gene for lOSb RNA (13). N2069 is a RNase III-strain derived from strain N7060 (18).Growth and Labeling of Cells. Cells were grown in lowph...

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mentioning

confidence: 99%

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

Gurevitz¹,

Jain²,

Apirion³

1983

Proc. Natl. Acad. Sci. U.S.A.

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“…The latter is therefore an internal promoter only with respect to the first coding sequence of the cluster, acting as a 5' flanking promoter with respect to the following coding regions. A multimeric transcript for tRNA genes is a very frequent phenomenon in prokaryotes (22). Here the promoter is located in the region flanking at the 5' side the cluster of coding sequences.…”

Section: Discussionmentioning

confidence: 99%

Transcription of multimeric tRNA genes

Ciliberto¹,

Traboni²,

Cortese³

1984

Nucl Acids Res

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We have constructed a set of plasmids carrying a tRNAPro gene from C. elegans in a head to tail dimeric and trimeric arrangement with virtually no spacer sequence. We show that in two different transcriptional systems, each coding region functions as an internal promoter directing the synthesis of independent transcriptional products. This is in contrast with the property of natural head to tail dimeric arrangements found in yeast, where only one coding region functions as promoter (Mao et al., 1980 Cell 20, 589; Schmidt et al., 1980 Nature 287, 750). The evolutionary significance of our finding is discussed.

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“…At least four types of processing reactions are involved in the maturation of prokaryotic and eukaryotic cytoplasmic tRNAs. These are (1) RNase P, which produces mature 5'-termini, (2) 3'-exonucleases and/or endonucleases which process at the 3'-termini, (3) 3'-tRNA nucleotidyltransferase (CCA-adding enzyme), and (4) specific base modifying enzymes (for reviews, see 1,2,17,36). However, little is known about the processing of chloroplast tRNA gene primary transcripts.…”

Section: Introductionmentioning

confidence: 99%

Accurate processing and pseudouridylation of chloroplast transfer RNA in a chloroplast transcription system

1984

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The trancription of a cloned trnV1-trnN1-trnR1 cluster from Euglena gracilis chloroplast (ct) DNA and the processing of a tRNA(Val)-tRNA(Asn)-tRNA(Arg) polycistronic precursor were studied in a spinach ct transcription extract. A soluble ct RNA polymerase selectively transcribes the trnV1-trnN1-trnR1-trnL1 locus in the EcoG fragment from the Euglena ct genome. Restriction enzyme modified templates and RNA fingerprint analysis were used to confirm that the tRNA genes were correctly transcribed. The tRNA(Val)-tRNA(Asn)-tRNA(Arg) polycistronic precursor transcribed by RNA polymerase III in a HeLa cell extract was used as a substrate to demonstrate that a ct tRNA precursor molecule is correctly processed by the ct tRNA processing enzymes. The oligonucleotide pattern of tRNAs processed in vitro from the tRNA(Val)-tRNA(Asn)-RNA(Arg) polycistronic precursor is indistinguishable from tRNA(Val), tRNA(Asn) and tRNA(Arg) transcribed by the ct RNA polymerase and processed in the ct transcription extract. The 3'-CCAOH is added to the tRNAs by a 3' nucleotidyltransferase after correct processing of the 3' terminus. Correct pseudouridylation was demonstrated for uridine residues in a tRNA(Met) m molecule transcribed from a spinach ct trnM1 locus. Thus, the enzymatic activities involved in tRNA biosynthesis in vitro include DNA-dependent (tDNA) RNA polymerase, a 5'-processing activity (RNase P-like), a 3'-exonuclease, an endoribonuclease involved in 3'-tRNA maturation, a tRNA nucleotidyltransferase, and pseudouridylate synthetase.

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Maturation Events Leading to Transfer RNA and Ribosomal RNA

Cited by 8 publications

References 312 publications

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

Transcription of multimeric tRNA genes

Accurate processing and pseudouridylation of chloroplast transfer RNA in a chloroplast transcription system

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