Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns

Yeung, Ching‐Hei; Oberländer, G.; Cooper, Trevor G.

doi:10.1002/mrd.1080380317

Cited by 24 publications

(16 citation statements)

References 26 publications

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“…Sulfhydryl groups of sperm membrane proteins maintain a dynamic equilibrium with their disul®des counterparts (Calvin et al, 1973). Oxidation of sulfhydryl groups is associated with the acquisition of fertilizing ability of spermatozoa (Yeung et al, 1994). In rat epididymis, caput ligation caused an oxidation of sperm sulfhydryl groups triggering an early maturation of the gametes as evidenced by the acquisition of motility and of a partial increase in fertilizing ability (Seligman et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione

Chatterjee¹,

Lamirande²,

Gagnon³

2001

Molecular Reproduction Devel

159

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Cryopreservation induces extensive biophysical and biochemical changes in the membrane of spermatozoa that ultimately decrease the fertility potential of the cells. Sulfhydryl groups of sperm proteins regulate a number of activities of the cells. Qualitative and quantitative analyses of sulfhydryl groups in the sperm membrane were performed by fluorescence microscopy, fluorimetry and electrophoresis. Fluorimetric analysis using 5-iodoacetamidofluoresceine indicated a two-fold increase in the content of sulfhydryl groups in sperm membrane after a freezing/thawing cycle. Electrophoresis of Triton-soluble sperm proteins after labeling with 3-(N-maleimidylpropionyl) biocytin indicated that proteins of 40-65 and 34 kDa range expose more sulfhydryl groups after cooling at 4 degrees C and freezing/thawing. Cryopreservation of spermatozoa changed the distribution pattern of sulfhydryl groups on sperm surface measured with fluorescence microscopy using 5-iodoacetamidofluoresceine. The percentage of spermatozoa labeled at the level of the mid-piece decreased by 50 and 90% after cooling and freezing/thawing, respectively. Spin labeling studies showed a 15% faster rotational diffusion (mobility) of sulfhydryl containing proteins in the membrane of frozen/thawed spermatozoa as compared to that of fresh spermatozoa. Addition of glutathione, reduced (GSH) or oxidized (GSSG), to the cryoprotectant partially prevented the effects of freezing/thawing, such as higher exposure of sulfhydryl groups, changes in the cellular distribution, and enhanced rotational diffusion of sulfhydryl containing proteins of sperm membrane. Addition of GSSG to the cryoprotectant reduced by 35% the loss of motility of spermatozoa undergoing a freezing/thawing cycle. We concluded that cryopreservation perturbs sperm membrane sulfhydryl containing proteins and that these modifications could be partially prevented by the addition of GSSG to the cryopreservation medium.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione

Chatterjee¹,

Lamirande²,

Gagnon³

2001

Molecular Reproduction Devel

159

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“…After attaining their maximal percentage motility and progressive velocity, slight but consistent decreases in motility of spermatozoa from the most distal epididymal region have been observed in hamsters and rats (Yeung et al, 1992(Yeung et al, , 1994b and are most obvious in men with prostatic carcinoma , probably due to the ageing of stored spermatozoa. In the present study this phenomenon of a decrease in percentage motility was only observed in the epididymis of one cynomolgus monkey.…”

Section: Discussionmentioning

confidence: 77%

Maturation of sperm motility in the epididymis of the common marmoset (Callithrix jacchus) and the cynomolgus monkey (Macaca fascicularis)

Yeung¹,

Morrell

Cooper³

et al. 1996

Int J of Andrology

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The acquisition by spermatozoa of the capacity for movement upon liberation from the epididymal duct into buffer is one of the obvious changes associated with sperm maturation and, hence, studies of sperm kinematics might provide good indices for sperm maturation. Profiles of development in the movement pattern of spermatozoa taken from six regions of the epididymis were established in marmoset and cynomolgus monkeys using computer-assisted sperm analysis. Motion of spermatozoa from initial regions was sluggish and irregular, then developed in the more distal region into erratic movement effected by an increase in the vigour of flagellation as reflected by curvilinear velocity. The tendency of immature spermatozoa to display circular motion was more common in the marmoset than in the cynomolgus monkey. In the marmoset the most drastic changes in percentage motility and kinematic parameters of spermatozoa occurred between the distal caput and the proximal corpus; maximal values for these parameters were found around the distal corpus epididymis. For the cynomolgus spermatozoa the sharpest gain in percentage motility was also achieved between the caput and proximal corpus epididymis, but with full kinematic development in the proximal cauda epididymis. These maturational changes were not synchronous among spermatozoa from the same site as they moved along the epididymis, as reflected in the heterogeneity of each sample in the kinematic properties of individual spermatozoa, especially those from the proximal corpus epididymis. The maturational profiles in the monkeys are comparable to the situation in the human, for which they may provide a useful model.

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“…The conditions and parameter set up values for the use of HTM-S Motility Analyzer for the analysis of hamster spermatozoa was as described earlier (Girija Devi and Shivaji, 1994) and was validated according to the guidelines of the HTM-S manual and as recom- mended by Yeung et al (1992) and Working and Hurtt (1987). A recent study has demonstrated that the settings validated for the analysis of rat spermatozoa also could be used for the analysis of hamster spermatozoa since these spermatozoa are very similar in size and shape (Yeung et al, 1994). The settings used in our previous study and in the present for hamster spermatozoa overlap with that reported by Yeung et al (1994) but are not identical.…”

Section: Discussionmentioning

confidence: 99%

“…With this in view, attempts had been made to standardize computer-aided methods for motility analysis of spermatozoa from humans (Robertson et al, 1987;Mack et al, 1988;Tesarik et al, 1990;Burkman, 1991), bull (Anzar et al, 1991), ram (Amann et al, 1982), and rat (Working and Hurtt, 1987;Slott et al, 1991;Yeung et al, 1992). Recently, a computer-aided motility analysis of hamster spermatozoa during maturation was described by us (Girija Devi and Shivaji, 1994) and the results were confirmed by Yeung et al (1994), who used a videorecording apparatus and the computer aided motion analysis system HTM 2030.…”

Section: Comparison Of the Distribution Of Hamster Spermatozoa Exhibimentioning

confidence: 98%

Analysis of the motility parameters of in vitro hyperactivated hamster spermatozoa

Shivaji

Peedicayil

Devi

1995

Molecular Reproduction Devel

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Golden hamster cauda epididymal spermatozoa under in vitro capacitating conditions exhibited time-dependent transformation of motility pattern, and the frequency of occurrence of a particular motility type was found to be dependent on the depth of the motility chamber used. The nonhyperactivated spermatozoa with planar motility were the most predominant at 0 hr irrespective of the depth of the motility chamber. But spermatozoa with the helical motility pattern were not detectable up to 6 hr when the Makler chamber was used, whereas in both the slide chamber and cannula, by 2 hr such spermatozoa constituted 90% of the total spermatozoa. However, by 6 hr the hyperactivated circular moving spermatozoa were the predominant type in all the chambers. Sperm motility chamber depths were also found to effect the motility parameter values of hamster spermatozoa, but this effect was also found to be dependent on the type of motility. Increase in chamber depth did not alter any of the motility parameter values of spermatozoa with hatchet type of motility and only increased the amplitude of lateral head displacement (ALH) in planar type. But in spermatozoa with the helical type of motility, an increase in chamber depth increased the progressive velocity (VSL), path velocity (VAP), curvilinear velocity (VCL), straightness (STR), linearity (LIN), and ALH. In spermatozoa with the circular type of motility, an increase in VSL, VAP, VCL, and ALH was also observed, but STR and LIN decreased. The hyperactivated spermatozoa could be distinguished from the nonhyperactivated spermatozoa because the former were swimming in circles, had low progressive velocity, decreased straightness and linearity of path, and also exhibited an increase in the amplitude of lateral head displacement compared to the nonhyperactivated spermatozoa. Further, the spermatozoa with helical motility could be differentiated from the nonhyperactivated, circular, and hatchet spermatozoa in that they had the highest VSL, VAP, VCL, and ALH. Spermatozoa with hatchet movement were slow and exhibited very low STR and LIN. Thus the motility parameters could be used to distinguish the nonhyperactivated and hyperactivated distal cauda epididymal spermatozoa.

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Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns

Cited by 24 publications

References 26 publications

Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione

Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione

Maturation of sperm motility in the epididymis of the common marmoset (Callithrix jacchus) and the cynomolgus monkey (Macaca fascicularis)

Analysis of the motility parameters of in vitro hyperactivated hamster spermatozoa

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