1999
DOI: 10.1155/2000/56106
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Maturation of Lymphocyte Immunophenotypes and Memory T Helper Cell Differentiation During Development in Mice

Abstract: The goal of this study was to systematically investigate the ontogeny of lymphoid populations throughout postnatal development. In CD-1 mice, peak lymphocyte numbers occurred in blood on postnatal day 10 (dl0) including those for natural killers (NKI.1), B cells (CD19), T helper (CD3CD4), naive T helper (CD4CD62LpSCD441w), memory T helper (CD4CD62LnegcD44high), and T cytotoxic (CD3CD8) cells. As percent of total lymphocytes, peaks were achieved by d 10 for all T helper subtypes but not B cells which declined t… Show more

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Cited by 7 publications
(14 citation statements)
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“…In contrast, in the total unsorted cell cultures this ratio could be calculated for B cells to CD3 cells only. The B:T cell ratios were: 11:1, 8:1, 4:1, 11:1, 4:1, 2:1 for 3-, 4-, 5-, 10-, 20-, and 45-day old pups, respectively (data obtained in a parallel immunophenotyping study; Fagoaga et al, 2001). Indeed, APC:T cell ratios are higher when monocytes and dendritic cells are taken into account.…”
Section: Discussionmentioning
confidence: 87%
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“…In contrast, in the total unsorted cell cultures this ratio could be calculated for B cells to CD3 cells only. The B:T cell ratios were: 11:1, 8:1, 4:1, 11:1, 4:1, 2:1 for 3-, 4-, 5-, 10-, 20-, and 45-day old pups, respectively (data obtained in a parallel immunophenotyping study; Fagoaga et al, 2001). Indeed, APC:T cell ratios are higher when monocytes and dendritic cells are taken into account.…”
Section: Discussionmentioning
confidence: 87%
“…Before day 5, there are very few memory cells in spleen, while adult spleens contain manyfold greater numbers (Fagoaga et al, 2001). Memory cells migrate to spleen after down-regulating CD62L expression (Forsthuber et al, 1996), and have increased capacity to produce Th1 and Th2 cytokines compared to naïve cells (Bradley et al, 1992).…”
Section: Discussionmentioning
confidence: 99%
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“…Whole blood and dispersed splenocytes were processed by flow cytometry to identify immunophenotypes according to previously described standard laboratory practices (Fagoaga et al, 2001). Leukocytes were manually counted (Unopette Cell Counting System, Fisher Scientific, Tustin, CA), and then lymphocyte preparations were labeled with murine-specific fluorochrome--tagged monoclonal antibodies (PharMingen, San Diego, CA).…”
Section: Methodsmentioning
confidence: 99%