Maturation of taste buds on the soft palate of the postnatal rat

Harada, Shuitsu; Yamaguchi, Keiko; Kanemaru, Norikazu; Kasahara, Yuuya

doi:10.1016/s0031-9384(99)00184-5

Cited by 54 publications

(46 citation statements)

References 13 publications

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“…A substantial number of pored and nonpored buds were demonstrated at E20 (i.e., about 60% of those recorded in adult; PN70). Harada et al (2000) reported that 53% of the taste buds in the SP contained a taste pore at birth, which is consistent with the present study. Our findings, however, contrast with Mistretta (1972) who recognized taste buds in the neonatal rats but concluded that they should be morphologically immature at birth.…”

Section: Temporal and Spatial Development Of The Palatal Taste Budssupporting

confidence: 93%

“…In support, ␣-gustducin, which is strongly implicated in the mediation of sweet-tasting substances, was strongly expressed in the palatal taste buds (Boughter et al, 1997). The present findings and interpretations on the previous investigations (Miller and Spangler, 1982;Harada et al, 2000) aroused great evidence about the gustatory functions of the palatal taste buds in the neonatal life and their temporal collaboration with contiguous taste bud populations. It is important to emphasize that the onset of sweet transduction in the rat began by the PN1 on the SP and PN4 on the NIP.…”

Section: Function Of the Palatal Taste Buds During Developmentsupporting

confidence: 79%

“…Settembrini (1987) recorded taste bud primordia in the newborn rats but confirmed that these should be regarded as morphologically mature by the second week of postnatal life. Harada et al (2000) recorded five to seven taste buds within the nasoincisor ducts at birth; one of which contained a taste pore. In the NIP of the mouse, OhtaYamakita et al (1982) stated that the taste buds appear at 4 or 5 days, matured at 10 days of age and there is a continuous taste bud formation in the papilla over the first 10 weeks of age.…”

Section: Temporal and Spatial Development Of The Palatal Taste Budsmentioning

confidence: 99%

“…Hall and Bryan (1981) stated that rats as young as 1-3 days of age shows differential responses to oral infusion of sucrose vs. water, which undoubtedly elucidates that functional taste buds should be present to mediate these ingestive responses. Contribution of the palatal taste buds to the taste mechanism in the neonatal rats became conceivably more crucial because Harada et al (2000) ascertained that 53% of the taste buds in the SP contained a taste pore at birth compared with only 14% within fungiform papillae; whereas no pores were observed within foliate and circumvallate papillae. In the present study, the onset of taste transduction triggered by the earliest ␣-gustducin-IR cells recognized at PN2 (1.6 Ϯ 0.5) and PN4 (1.4 Ϯ 0.5) in the SP and NIP, respectively.…”

Section: Taste Transduction and Morphological Maturation Of The Palatmentioning

confidence: 99%

“…Moreover, their spatial distribution is functionally important for the suckling behavior in pre-weanling animals as milk stimulates them much more than the lingual taste buds (Ardran et al, 1958). In the neonatal rat, Harada et al (2000) assigned that the number of pores in the SP of the rat at birth is significantly higher than the lingual taste bud populations, which reflects the crucial contribution of palatal taste buds in the mediation of taste directly after birth. In contrast to the contiguous taste bud populations, whether those of the palate begin their development prenatally or postnatally has not previously elucidated.…”

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confidence: 99%

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Development and maturation of taste buds of the palatal epithelium of the rat: Histological and immunohistochemical study

et al. 2001

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Palatal taste buds are intriguing partners in the mediation of taste behavior and their spatial distribution is functionally important for suckling behavior, especially in the neonatal life. Their prenatal development has not been previously elucidated in the rat, and the onset of their maturation remains rather controversial. We delineated the development and frequency distribution of the taste buds as well as the immunohistochemical expression of ␣-gustducin, a G protein closely related to the transduction of taste stimuli, in the nasoincisor papilla (NIP) and soft palate (SP) from the embryonic day 17 (E17) till the postnatal day 70 (PN70). The main findings in the present study were the development of a substantial number of taste pores in the SP of fetal rats (60.3 Ϯ 1.7 out of 122.8 Ϯ 5.5; mean Ϯ SD/animal at E19) and NIP of neonatal rats (9.8 Ϯ 1.0 out of 44.8 Ϯ 2.2 at PN4). ␣-gustducin-like immunoreactivity (-LI) was not expressed in the pored taste buds of either prenatal or newborn rats. The earliest expression of ␣-gustducin-LI was demonstrated at PN1 in the SP (1.5 Ϯ 0.5 cells/taste bud; mean Ϯ SD) and at PN4 in the NIP (1.4 Ϯ 0.5). By age the total counts of pored taste buds continuously increased and their morphological features became quite discernible. They became pear in shape, characterized by distinct pores, long subporal space, and longitudinally oriented cells. Around the second week, a remarkable transient decrease in the total number of taste buds was recorded in the oral epithelium of NIP and SP, which might be correlated with the changes of ingestive behaviors. The total counts of cells showing ␣-gustducin-LI per taste bud gradually increased till the end of our investigation (14.1 Ϯ 2.7 in NIP and 12.4 Ϯ 2.5 in SP at PN70). We conclude that substantial development of taste buds began prenatally in the SP, whereas most developed entirely postnatal in the NIP. The present study provides evidence that the existence of a taste pore which is considered an important criterion for the morphological maturation of taste buds is not enough for the onset of the taste transduction, which necessitates also mature taste cells. Moreover, the earlier maturation of palatal taste buds compared with the contiguous populations in the oral cavity evokes an evidence of their significant role in the transmission of gustatory information, especially in the early life of rat. Anat Key words: taste buds; development; ␣-gustducin; nasoincisor papilla; soft palate; rat Taste transduction is arbitrated by specialized neuroepithelial cells, referred to as gustatory or taste cells that organize into groups forming taste buds of which the vast majority are embedded within the lingual and palatal epithelium. Since Hoffmann (1875) has reported the existence of taste buds on the human palatal mucosa, numerous investigations have been conducted to investigate their spatial distribution rather than gustatory functions. The earliest accounts in the rat were published by Kolmer (1927) and Kaplick (1953) who identifie...

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Section: Temporal and Spatial Development Of The Palatal Taste Budssupporting

confidence: 93%

Section: Function Of the Palatal Taste Buds During Developmentsupporting

confidence: 79%

Section: Temporal and Spatial Development Of The Palatal Taste Budsmentioning

confidence: 99%

Section: Taste Transduction and Morphological Maturation Of The Palatmentioning

confidence: 99%

mentioning

confidence: 99%

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Development and maturation of taste buds of the palatal epithelium of the rat: Histological and immunohistochemical study

et al. 2001

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Gustatory terminal field organization and developmental plasticity in the nucleus of the solitary tract revealed through triple‐fluorescence labeling

May

Hill

2006

J of Comparative Neurology

122

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Early dietary sodium restriction has profound influences on the organization of the gustatory brainstem. However, the anatomical relationships among multiple gustatory nerve inputs have not been examined. Through the use of triple-fluorescence labeling and confocal laser microscopy, terminal fields of the greater superficial petrosal (GSP), chorda tympani (CT), and glossopharyngeal (IX) nerves were visualized concurrently in the nucleus of the solitary tract (NTS) of developmentally sodium-restricted and control rats. Dietary sodium restriction during pre- and postnatal development resulted in a twofold increase in the volume of both the CT and the IX nerve terminal fields but did not affect the volume of the GSP terminal field. In controls, these nerve terminal fields overlapped considerably. The dietary manipulation significantly increased the overlapping zones among terminal fields, resulting in an extension of CT and IX fields past their normal boundaries. The differences in terminal field volumes were exaggerated when expressed relative to the respective NTS volumes. Furthermore, increased terminal field volumes could not be attributed to an increase in the number of afferents because ganglion cell counts did not differ between groups. Taken together, selective increases in terminal field volume and ensuing overlap among terminal fields suggest an increased convergence of these gustatory nerve terminals onto neurons in the NTS. The genesis of such convergence is likely related to disruption of cellular and molecular mechanisms during the development of individual terminal fields, the consequences of which have implications for corresponding functional and behavioral alterations.

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Overexpression of neurotrophin 4 in skin enhances myelinated sensory endings but does not influence sensory neuron number

Krimm

Davis

Noel

et al. 2006

J of Comparative Neurology

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The growth factors neurotrophin 4 (NT4) and brain-derived neurotrophic factor (BDNF) are expressed in the developing skin, activate the trkB tyrosine kinase receptor, and influence the development and survival of specific types of sensory afferents. Whether each factor is capable of regulating the same or overlapping populations of cutaneous afferents during development is unknown. A previous study of mice overexpressing BDNF in the developing skin (BDNF-OE mice) revealed that these animals exhibited increased hair follicle innervation, Meissner corpuscle size, and Merkel cell number in glabrous skin although no change in the total number of sensory neurons was observed. To determine if NT4 affects cutaneous innervation in a manner similar to BDNF, transgenic mice overexpressing NT4 in skin, under the control of the keratin 14 gene promoter, were examined. Similar to BDNF-OE mice, NT4-OE mice had increased innervation to the skin but no increase in sensory neuron number in either the dorsal root ganglion or trigeminal ganglion. NT4 overexpression also enhanced hair follicle innervation and the size and density of innervation to Meissner corpuscles. Unlike BDNF overexpression, NT4 overexpression did not alter the number of Merkel cells in the glabrous skin, but it did enhance the number of myelinated axons in nerves projecting to skin. Thus, the same pattern of BDNF and NT4 overexpression within the skin produces phenotypes that are both similar and distinctive.

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Maturation of taste buds on the soft palate of the postnatal rat

Cited by 54 publications

References 13 publications

Development and maturation of taste buds of the palatal epithelium of the rat: Histological and immunohistochemical study

Development and maturation of taste buds of the palatal epithelium of the rat: Histological and immunohistochemical study

Gustatory terminal field organization and developmental plasticity in the nucleus of the solitary tract revealed through triple‐fluorescence labeling

Overexpression of neurotrophin 4 in skin enhances myelinated sensory endings but does not influence sensory neuron number

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