Maturation of the Infant Respiratory Microbiota, Environmental Drivers, and Health Consequences. A Prospective Cohort Study

Bosch, Astrid A. T. M.; Piters, Wouter A A de Steenhuijsen; Houten, Marlies A. van; Chu, Mei Ling J N; Biesbroek, Giske; Kool, Jolanda; Pernet, Paula; Groot, Pieter-Kees C. M. de; Eijkemans, Marinus J.C.; Keijser, Bart J. F.; Sanders, Elisabeth A. M.; Bogaert, Debby

doi:10.1164/rccm.201703-0554oc

Cited by 267 publications

(388 citation statements)

References 32 publications

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“…In a cohort of 234 Australian infants with a high risk of atopy, Teo and colleagues reported that early Moraxella -dominant nasopharyngeal microbiota was associated with an earlier first upper respiratory infection 11. Similarly, in a cohort of 112 children in the Netherlands, Bosch and colleagues reported an association between enrichment of Moraxella in early life in children and a high frequency of ARIs during the first year of life 9. In contrast, in an analysis of trial data in the Netherlands (n=60), Biesbroek and colleagues found that infants with Moraxella -dominant nasopharyngeal microbiota had a lower risk of subsequent ARI 7.…”

Section: Discussionmentioning

confidence: 96%

Early nasal microbiota and acute respiratory infections during the first years of life

Toivonen

Hasegawa

Waris

et al. 2019

Thorax

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BackgroundEmerging evidence shows that airway microbiota may modulate local immune responses, thereby contributing to the susceptibility and severity of acute respiratory infections (ARIs). However, there are little data on the longitudinal relationships between airway microbiota and susceptibility to ARIs in children.ObjectiveWe aimed to investigate the association of early nasal microbiota and the subsequent risk of ARIs during the first years of life.MethodsIn this prospective population-based birth-cohort study in Finland, we followed 839 healthy infants for ARIs from birth to age 24 months. Nasal microbiota was tested using 16S rRNA gene sequencing at age 2 months. We applied an unsupervised clustering approach to identify early nasal microbiota profiles, and examined the association of profiles with the rate of ARIs during age 2–24 months.ResultsWe identified five nasal microbiota profiles dominated by Moraxella, Streptococcus, Dolosigranulum, Staphylococcus and Corynebacteriaceae, respectively. Incidence rate of ARIs was highest in children with an early Moraxella-dominant profile and lowest in those with a Corynebacteriaceae-dominant profile (738 vs 552/100 children years; unadjusted incidence rate ratio (IRR), 1.34; 95% CI 1.16 to 1.54; p < 0.001). After adjusting for nine potential confounders, the Moraxella-dominant profile-ARI association persisted (adjusted IRR (aIRR), 1.19; 95% CI 1.04 to 1.37; p = 0.01). Similarly, the incidence rate of lower respiratory tract infections (a subset of all ARIs) was significantly higher in children with an early Moraxella-dominant profile (aIRR, 2.79; 95% CI 1.04 to 8.09; p = 0.04).Conclusion Moraxella-dominant nasal microbiota profile in early infancy was associated with an increased rate of ARIs during the first 2 years of life.

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Section: Discussionmentioning

confidence: 96%

Early nasal microbiota and acute respiratory infections during the first years of life

Toivonen

Hasegawa

Waris

et al. 2019

Thorax

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“…16S-rRNA sequencing. Amplicon libraries of the 16S-rRNA gene (V4 region) were generated, and sequencing was executed as previously described 7 . Amplicon pools were paired-end sequenced in seven runs using an Illumina MiSeq instrument (Illumina Inc., San Diego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Staphylococcus epidermidis (2) Corynebacterium propinquum (3) Dolosigranulum (4) Moraxella (6) Streptococcus (7) Peptoniphilus (5) Finegoldia (8) Haemophilus (9) Fusobacterium (10)…”

Section: Corynebacterium (1)unclassified

“…Both initial colonization and subsequent dynamics are likely to be impacted by the local microbiota through a process called colonization resistance, which can be direct or immune mediated 4,5 . This concept of colonization resistance may likely also prevent pathogen overgrowth in the respiratory tract 6,7 , and may therefore have a causal role on pneumococcal colonization and elimination.…”

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confidence: 99%

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Interaction between the nasal microbiota and S. pneumoniae in the context of live-attenuated influenza vaccine

et al. 2019

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Streptococcus pneumoniae is the main bacterial pathogen involved in pneumonia. Pneumococcal acquisition and colonization density is probably affected by viral co-infections, the local microbiome composition and mucosal immunity. Here, we report the interactions between live-attenuated influenza vaccine (LAIV), successive pneumococcal challenge, and the healthy adult nasal microbiota and mucosal immunity using an experimental human challenge model. Nasal microbiota profiles at baseline are associated with consecutive pneumococcal carriage outcome (non-carrier, low-dense and high-dense pneumococcal carriage), independent of LAIV co-administration. Corynebacterium / Dolosigranulum -dominated profiles are associated with low-density colonization. Lowest rates of natural viral co-infection at baseline and post-LAIV influenza replication are detected in the low-density carriers. Also, we detected the fewest microbiota perturbations and mucosal cytokine responses in the low-density carriers compared to non-carriers or high-density carriers. These results indicate that the complete respiratory ecosystem affects pneumococcal behaviour following challenge, with low-density carriage representing the most stable ecological state.

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“…Variation in community composition has been observed in chronic lung diseases,6 7 and particular micro-organisms or combinations of organisms in infants have been associated with future disease development. Thus deviation from a healthy microbiota in early life appears to play an important role in disease development, with changes evident by 1 week of age 8. The dynamics of the infant airway microbiota and its relationship to disease development has therefore been the focus of increasing attention.…”

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confidence: 99%

Growing up with your airway microbiota: a risky business

Ahmed

Cox

Cuthbertson

2019

Thorax

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Maturation of the Infant Respiratory Microbiota, Environmental Drivers, and Health Consequences. A Prospective Cohort Study

Cited by 267 publications

References 32 publications

Early nasal microbiota and acute respiratory infections during the first years of life

Early nasal microbiota and acute respiratory infections during the first years of life

Interaction between the nasal microbiota and S. pneumoniae in the context of live-attenuated influenza vaccine

Growing up with your airway microbiota: a risky business

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