Maximization of fructooligosaccharide production by two stage continuous process and its scale up

Sangeetha, P.T.; Ramesh, M.N.; Prapulla, S. G.

doi:10.1016/j.jfoodeng.2004.05.022

Cited by 57 publications

(44 citation statements)

References 10 publications

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“…FOS are mainly composed of 1-kestose, 1-nystose, and 1-b-fructofuranosyl nystose, which can be found in many fruits and vegetables, although in very low concentrations to exert any beneficial effect, and its production is limited by seasonal conditions [1,4]. Thus, extraction procedures to obtain these compounds are not viable, and the FOS production on an industrial level is required.…”

Section: Introductionmentioning

confidence: 99%

Production of fructooligosaccharides and β-fructofuranosidase by batch and repeated batch fermentation with immobilized cells of Penicillium expansum

Mussatto

Prata

Rodrigues

et al. 2012

Eur Food Res Technol

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The production of fructooligosaccharides (FOS) and b-fructofuranosidase (FFase) by immobilized cells of Penicillium expansum was evaluated. In an initial stage, different low-cost materials including synthetic fiber, polyurethane foam, stainless steel sponge, loofah sponge, and cork oak were tested as carrier for the fungus immobilization. Additionally, the influence of the inoculum age (1 or 3 weeks) on cells immobilization, FOS and FFase production was also verified. Synthetic fiber and polyurethane foam were the best materials for P. expansum immobilization (2.21 and 1.98 g/g carrier, respectively) and FOS production (120.3 and 104.8 g/l), and gave also high results of FFase activity (23.01 and 32.42 U/ml). Then, the production of FOS and FFase by repeated batch fermentation with P. expansum immobilized on synthetic fiber was studied, aiming to improve the batch fermentation results. The results obtained in this stage were very promising with FOS yields of 87, 72, and 44 %, in the 3 initial cycles (60 h), respectively; the FFase activity was constant throughout the process (6 cycles, 96 h). Repeated batch fermentation with immobilized cells of P. expansum was found as being a technology with great potential for FOS and FFase production on industrial scale.

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Section: Introductionmentioning

confidence: 99%

Production of fructooligosaccharides and β-fructofuranosidase by batch and repeated batch fermentation with immobilized cells of Penicillium expansum

Mussatto

Prata

Rodrigues

et al. 2012

Eur Food Res Technol

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“…Conventionally, FOS production is a two-stage process in which the Wrst stage is the microbial production of the enzyme by fermentation and the second stage is the enzyme reaction with sucrose (substrate) to produce FOS under controlled conditions [8]. There are a few reports on the production of FOS in a single step, i.e., based on the production of the enzyme and the enzymatic reaction in one unique fermentation process.…”

Section: Introductionmentioning

confidence: 99%

β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus

Mussatto

Rodrigues

Teixeira

2009

J Ind Microbiol Biotechnol

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The fungus Aspergillus japonicus ATCC 20236 was immobilized in vegetal fiber and used in repeated batch fermentations of sucrose (200 g/l) for the production of beta-fructofuranosidases (FFase). The assays were performed during eight consecutive cycles that were completed in a total period of 216 h. After each 24-h cycle of fermentation (except for the first cycle, which lasted 48 h), the fermented broth was replaced by fresh medium, and the FFase activity was determined in the replaced medium. The average value of FFase activity was a constant 40.6 U/ml at the end of the initial seven cycles, but had decreased by 22% at the end of the eighth cycle. Concurrent with these high and constant FFase values, the hydrolyzing activity of this enzyme increased during the cycles, while the transfructosylating activity decreased. As a consequence, the maximum production of fructooligosaccharides of 134.60 g/l observed in the initial 30 h of fermentation (first cycle) had gradually decreased by the end of the subsequent cycles, reaching approximately 23% of this value during cycles 4-8. Based on these results, we conclude that the present immobilization system has a great potential for application in a semi-continuous process for the production of FFase, but further studies are necessary to maintain the FFase transfructosylation activity at high levels during the overall process.

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“…The eight components that were contained in the feed should be ideally separated into the raffinate containing 1-kestose, 1-nystose, 1 F -fructofuranosyl nystose, GF 5 and GF 6 and the extract consisting of fructose, glucose and sucrose. Considering the close values of the distribution coefficients of 1-kestose and sucrose and the strong column dispersion, a complete separation of FOS from glucose, fructose and sucrose would result in very high requirements for the designed SMB chromatography process.…”

Section: Resultsmentioning

confidence: 99%

“…Due their beneficial effects on human health, which were described in many studies, both their daily consumption and their industrial production are growing [3][4][5]. The reaction mixture contains high amounts of FOS (1-kestose, 1-nystose, 1 F -fructofuranosyl nystose, and higher FOS) and glucose and a small portion of fructose and unreacted sucrose [6]. Due to the presence of mono-and disaccharides, which have caloric and cariogenic effects, separation of the FOS is necessary.…”

Section: Introductionmentioning

confidence: 99%

Design of Fructooligosaccharide Separation Using Simulated Moving‐Bed Chromatography

Vaňková

Polakovič

2011

Chem Eng & Technol

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Design simulations of a simulated moving-bed (SMB) chromatography unit for the separation of fructooligosaccharides (FOS) were carried out. Using the constraints on the FOS purity in the raffinate of 90 % and FOS yield in the raffinate higher than 95 %, optimal distribution of a system of twelve columns into individual process zones and optimal values for the port switching period and the flow rates were found. The optimization function was the maximum productivity of FOS when the other evaluated performance criteria were the eluent consumption and product dilution. The performance of the designed SMB system was compared to that of a batch chromatography system that was simulated for a battery of identical columns. The sensitivity of the found optimum was analyzed with respect to the optimization parameters.

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Maximization of fructooligosaccharide production by two stage continuous process and its scale up

Cited by 57 publications

References 10 publications

Production of fructooligosaccharides and β-fructofuranosidase by batch and repeated batch fermentation with immobilized cells of Penicillium expansum

Production of fructooligosaccharides and β-fructofuranosidase by batch and repeated batch fermentation with immobilized cells of Penicillium expansum

β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus

Design of Fructooligosaccharide Separation Using Simulated Moving‐Bed Chromatography

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