Maximum rate of oxygen consumption related to succinate dehydrogenase activity in skeletal muscle fibres of chronic heart failure patients and controls

Bekedam, Martijn A.; Beek-Harmsen, Brechje J. van; Boonstra, Anco; Mechelen, Willem van; Visser, Frans C.; Laarse, Willem J. van der

doi:10.1046/j.1475-0961.2003.00517.x

Cited by 44 publications

(34 citation statements)

References 39 publications

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“…These results fit well to the extrapolated relationship determined previously using different types of single skeletal muscle fibers (van der Laarse et al, 1989). Figure 2 also contains measurements of VO 2,max during a bicycle exercise test and SDH activity measured in biopsies of the vastus lateralis muscle (Bekedam et al, 2002). We conclude on the basis of the results on rat trabeculae and human biopsies that SDH activity determined histochemically allows an estimate of the oxidative capacity of mammalian striated muscle cells.…”

Section: Resultssupporting

confidence: 86%

“…The cross-sectional area of these preparations was smaller than 0.035 mm 2 , which is a requirement to prevent hypoxia during these measurements. Open circle: VO 2max of six human subjects, measured using an incremental bicycle exercise test, and mean SDH activity measured in biopsies of the vastus lateralis muscle (data from Bekedam et al, 2002). The s.d.…”

Section: Resultsmentioning

confidence: 99%

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Calibrated histochemistry applied to oxygen supply and demand in hypertrophied rat myocardium

Tombe

Beek-Harmsen

Groot

et al. 2002

Microscopy Res & Technique

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Oxygen supply and demand of individual cardiomyocytes during the development of myocardial hypertrophy is studied using calibrated histochemical methods. An oxygen diffusion model is used to calculate the critical extracellular oxygen tension (PO(2,crit)) required by cardiomyocytes to prevent hypoxia during hypertrophic growth, and determinants of PO(2,crit) are estimated using calibrated histochemical methods for succinate dehydrogenase activity, cardiomyocyte cross-sectional area, and myoglobin concentration. The model calculation demonstrates that it is essential to calibrate the histochemical methods, so that absolute values for the relevant parameters are obtained. The succinate dehydrogenase activity, which is proportional to the maximum rate of oxygen consumption, and the myoglobin concentration hardly change while the cardiomyocytes grow. The cross-sectional area of the cardiomyocytes, which increases up to threefold in the right ventricular wall due to pulmonary hypertension in monocrotaline-treated rats, is the most important determinant of PO(2,crit) in this model of myocardial hypertrophy. The relationship between oxygen supply and demand at the level of the cardiomyocyte can be investigated using paired determinations of spatially integrated succinate dehydrogenase activity and capillary density. Hypoxia-inducible factor 1alpha can be demonstrated by immunohistochemistry in cardiomyocytes with high PO(2,crit) and increased spatially integrated succinate dehydrogenase activity, indicating that limited oxygen supply affects gene expression in these cells. We conclude that a mismatch of oxygen supply and demand may develop during hypertrophic growth, which can play a role in the transition from myocardial hypertrophy to heart failure.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Calibrated histochemistry applied to oxygen supply and demand in hypertrophied rat myocardium

Tombe

Beek-Harmsen

Groot

et al. 2002

Microscopy Res & Technique

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“…To our knowledge, it has never been tested whether, and to what extent, trained humans are able to increase iSDH activity values. This study shows that athletes succeeded in obtaining much greater iSDH activity values (0.147 6 0.034 in DA 660 mm/s) than untrained humans do [0.087 6 0.004 (50)] or than patients with heart failure do [0.048 6 0.004 (50)]. So, even though muscle fiber size and oxidative capacity were inversely related, athletes were able to obtain high iSDH activity values.…”

Section: Physiology Of Sprinters: High Normalized Sprint Performance mentioning

confidence: 75%

Critical determinants of combined sprint and endurance performance: an integrative analysis from muscle fiber to the human body

et al. 2018

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Optimizing physical performance is a major goal in current physiology. However, basic understanding of combining high sprint and endurance performance is currently lacking. This study identifies critical determinants of combined sprint and endurance performance using multiple regression analyses of physiologic determinants at different biologic levels. Cyclists, including 6 international sprint, 8 team pursuit, and 14 road cyclists, completed a Wingate test and 15-km time trial to obtain sprint and endurance performance results, respectively. Performance was normalized to lean body mass 2/3 to eliminate the influence of body size. Performance determinants were obtained from whole-body oxygen consumption, blood sampling, knee-extensor maximal force, muscle oxygenation, wholemuscle morphology, and muscle fiber histochemistry of musculus vastus lateralis. Normalized sprint performance was explained by percentage of fast-type fibers and muscle volume (R 2 = 0.65; P < 0.001) and normalized endurance performance by performance oxygen consumption (Vȯ 2 ), mean corpuscular hemoglobin concentration, and muscle oxygenation (R 2 = 0.92; P < 0.001). Combined sprint and endurance performance was explained by gross efficiency, performance Vȯ 2 , and likely by muscle volume and fascicle length (P = 0.056; P = 0.059). High performance Vȯ 2 related to a high oxidative capacity, high capillarization 3 myoglobin, and small physiologic cross-sectional area (R 2 = 0.67; P < 0.001). Results suggest that fascicle length and capillarization are important targets for training to optimize sprint and endurance performance simultaneously.-Van der Zwaard, S., van der Laarse, W. J., Weide, G., Bloemers, F. W., Hofmijster, M. J., Levels, K., Noordhof, D. A., de Koning, J. J., de Ruiter, C. J., Jaspers, R. T. Critical determinants of combined sprint and endurance performance: an integrative analysis from muscle fiber to the human body. FASEB J. 32, 2110FASEB J. 32, -2123FASEB J. 32, (2018 Many sports require a combination of sprint and endurance performance. During the past decades, physiologic determinants of physical performance have been the subject of intensive investigation (e.g., in cycling, 1-11). Studies focused on determinants of either sprint (e.g., 8-18) or endurance performance (e.g., 1-7, 19-23), even though physical performance is rarely a dichotomous function of only sprint or only endurance. Generally, a limited number of whole-body determinants of sprint or endurance performance have been studied, although there are many physical performance determinants at different levels (i.e., molecular, cellular, whole-muscle, organ, and whole ABBREVIATIONS: 3D, 3-dimensional; CAF, capillaries around the fiber; CD, capillary density; C/F, capillary-to-fiber ratio; FCSA, fiber cross-sectional area; fVȯ 2max , fiber maximal oxygen consumption; [Hb], hemoglobin concentration; Hct, hematocrit; [HHbMb], deoxygenated hemoglobin and myoglobin concentration; iSDH activity, spatially integrated SDH activity, SDH activity 3 FCSA; KE, k...

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“…, the M O2,max in a domain can be calculated in pl min −1 mm −1 and volume-specific M O2,max in ml kg −1 min −1 (Bekedam et al, 2003).…”

Section: Morphometrymentioning

confidence: 99%

“…This correlation between anatomical capillary supply and tissue oxidative capacity also seems to apply at a smaller scale of biological control, where the local capillary supply to an individual fibre appears to be positively related to its oxidative capacity (Bekedam et al, 2003). Importantly, in such studies, fibre size was not considered and hence the influence of local diffusion distances on cellular oxygenation cannot be assessed.…”

Section: Introductionmentioning

confidence: 99%

Local capillary supply in muscle is not determined by local oxidative capacity

Bosutti

Egginton

Barnouin

et al. 2015

Journal of Experimental Biology

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It is thought that the prime determinant of global muscle capillary density is the mean oxidative capacity. However, feedback control during maturational growth or adaptive remodelling of local muscle capillarisation is likely to be more complex than simply matching O 2 supply and demand in response to integrated tissue function. We tested the hypothesis that the maximal oxygen consumption (M O2,max ) supported by a capillary is relatively constant, and independent of the volume of tissue supplied (capillary domain). We demonstrate that local M O2,max assessed by succinate dehydrogenase histochemistry: (1) varied more than 100-fold between individual capillaries and (2) was positively correlated to capillary domain area in both human vastus lateralis (R=0.750, P<0.001) and soleus (R=0.697, P<0.001) muscles. This suggests that, in contrast to common assumptions, capillarisation is not primarily dictated by local oxidative capacity, but rather by factors such as fibre size, or consequences of differences in fibre size such as substrate delivery and metabolite removal.

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Maximum rate of oxygen consumption related to succinate dehydrogenase activity in skeletal muscle fibres of chronic heart failure patients and controls

Cited by 44 publications

References 39 publications

Calibrated histochemistry applied to oxygen supply and demand in hypertrophied rat myocardium

Calibrated histochemistry applied to oxygen supply and demand in hypertrophied rat myocardium

Critical determinants of combined sprint and endurance performance: an integrative analysis from muscle fiber to the human body

Local capillary supply in muscle is not determined by local oxidative capacity

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