MC3T3‐E1 and RAW264.7 cell response to hydroxyapatite and alpha‐type alumina adsorbed with bovine serum albumin

Abstract

Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2O3, but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al2O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2O3. Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity.

Protein Adsorption to Titanium and Zirconia Using a Quartz Crystal Microbalance Method

“…On the contrary, albumin is a major component in the human saliva and is known as cell adhesion-inhibiting protein. Kawashita et al reported that albumin adsorbed hydroxyapatite and alumina inhibited the adhesion and spreading of MC3T3-E1 cells [ 15 ]. Thus, it is important to observe protein adsorption to Ti and ZrO 2 to identify biological properties of Ti and ZrO 2 .…”

In vitro evaluation of biocompatibility of Ti–Mo–Sn–Zr superelastic alloy

“…In addition, FDA staining was performed in this study for the quantitative evaluation of cell growth by fluorescent staining and visual observation of live cells grown on disk samples as described in Kawashita et al. 51 Results obtained for both V79 and MC3T3-E1 cell lines confirmed that cell growth increased over time on both TMSZ and cpTi. It was confirmed that TMSZ showed biocompatibility equivalent to that of cpTi for the growth of V79 and MC3T3-E1 cells.…”

The Application of Multi-Walled Carbon Nanotubes in Bone Tissue Repair Hybrid Scaffolds and the Effect on Cell Growth In Vitro

“…Furthermore, their biocompatibility, biodegradability, low immunogenicity and cost, and their ability to support cell adhesion, proliferation, and differentiation [22,23,24,25,26] mean they have significant potential in bone tissue engineering [26,27,28,29,30]. Osteoconductivity is a significant property of biomaterials which chemically bonds to bone and supports bone formation [31]. Hydroxyapatite (HAp), a major component of bone mineral, is an osteoconductive biomaterial [31,32,33] that has been generally used to make scaffolds for bone tissue engineering [34,35,36,37,38,39].…”

“…Osteoconductivity is a significant property of biomaterials which chemically bonds to bone and supports bone formation [31]. Hydroxyapatite (HAp), a major component of bone mineral, is an osteoconductive biomaterial [31,32,33] that has been generally used to make scaffolds for bone tissue engineering [34,35,36,37,38,39]. Previously, a chitosan/alginate/SiO 2 (CS/Alg/SiO 2 ) composite scaffold prepared by freeze-drying was demonstrated to have a 3D microporous structure, controllable biodegradation, and improved apatite deposition performance that enhanced bone tissue repair [34].…”

“…CNTs have a surface roughness and surface area similar to collagen fibers in the extracellular matrix, which can greatly promote cell attachment, proliferation, and differentiation [41,42]. In recent years, the MC3T3-E1 cell, an osteoblast cell from a mouse, has been generally used as a cell source for osteogenic differentiation [31,32,38,43]. In this study, CS/Gel/nHAp/MWCNTs scaffolds were prepared by freeze-drying, and the impact of varying MWCNT content on the physicochemical properties and biocompatibility of the scaffold was investigated.…”