Measles virus-induced suppression of lymphocyte reactivity in vitro

Lucas, C.J.; Galama, J.M.D.; Ubels-Postma, José

doi:10.1016/0008-8749(77)90183-6

Cited by 33 publications

(16 citation statements)

References 12 publications

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“…In contrast to the response obtained when PBLs were incubated with PWM, no increased proliferation of either infected or uninfected lymphocytes occurred when these cells were cultured with either the control or the anti-interferon serum. The response to PWM was reduced in stimulated measles virus-infected lymphocytes as had been reported previously (Lucas et al, 1977). We conclude from these data that the ability of anti-human leukocyte interferon to induce high levels of infectious measles virus from resting, virus-infected lymphocytes (Fig.…”

Section: Ability Of Anti-human Leukocyte Interferon To Act As a Mitogensupporting

confidence: 88%

Measles Virus Persistence in Human Lymphocytes: A Role for Virus-induced Interferon

Jacobson

McFarland

1982

Journal of General Virology

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Section: Ability Of Anti-human Leukocyte Interferon To Act As a Mitogensupporting

confidence: 88%

Measles Virus Persistence in Human Lymphocytes: A Role for Virus-induced Interferon

Jacobson

McFarland

1982

Journal of General Virology

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“…A low or absent proliferation after PHA stimulation of PBL chronically infected by virus in vitro has been described [13]. The virus became demonstrable first after PHA stimulation.…”

Section: Discussionmentioning

confidence: 96%

Reduced in Vitro Response of CSF Lymphocytes to Mitogen Stimulation in Multiple Sclerosis

et al. 1979

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By means of a microculture technique and calculation of incorporation of 14C-thymidine, cerebrospinal fluid (CSF) lymphocytes from multiple sclerosis (MS) patients showed low or absent proliferation when stimulated with phytohaemagglutinin, concanavalin A, or pokeweed mitogen, in contrast to peripheral blood lymphocytes (PBL) obtained simultaneously and investigated in parallel. A lower proliferation of CSF lymphocytes compared with PBL was also found in acute aseptic meningitis, although it has been reported that CSF lymphocytes show greater proliferation than PBL when specifically stimulated. The low proliferation of MS CSF lymphocytes on mitogen stimulation may be a consequence of prolonged sensitization to an as yet unidentified antigen. The proliferation of MS CSF lymphocytes was not improved by adding irradiated PBL, making helper cell insufficiency less likely. MS CSF had no inhibitory effect on proliferation of PBL, arguing against an inhibitory effect of soluble factors in the CSF as an explanation for the depressed response of CSF lymphocytes.

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“…The infection of peripheral blood mononuclear (PBM) 0000-7871 © 1988 SGM cells in vivo leads to a silent infection, but mitogenic stimulation of lymphocytes leads to virus replication and release of infectious virus. This is associated with a strong inhibition of cell proliferation and ultimately with cell death (Sullivan et al, 1975a, b;Lucas et al, 1977). Most studies on the infection of human lymphoid cells have been done using the Edmonston strain of the virus.…”

Section: Measles Virus Strain-dependent Variation In Outcome Ofmentioning

confidence: 99%

Measles Virus Strain-dependent Variation in Outcome of Infection of Human Blood Mononuclear Cells

Ilonen

Salonen

Marusyk

et al. 1988

Journal of General Virology

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SUMMARYEight measles virus strains including four subacute sclerosing panencephalitis (SSPE) isolates were compared on the basis of their growth characteristics in human peripheral blood mononuclear cells and effect on mitogen-stimulated lymphocyte proliferation. The Edmonston strain virus and some other strains of measles virus replicated in phytohaemagglutinin (PHA)-stimulated lymphocytes and released high titres of infectious virus into the culture medium. Inhibition of DNA synthesis was relatively low at the beginning of lymphocyte proliferation and could be detected only at high multiplicity of infection. Three to 4 days after initiation of proliferation a strong inhibition was seen also in cultures infected with low doses of virus, and was apparently related to the production of high titres of new infectious virus. Some virus strains originally isolated from SSPE patients produced only a small amount of infectious virus in lymphocytes at 37 °C but had a very strong inhibitory effect on lymphocyte proliferation leading to early cell death. The inhibitory effect was found over a wide range of virus concentrations, but was strictly dose-dependent and no increase in inhibition with low multiplicities of infection could be seen with longer culture times. The amount of interferon induced by different strains varied from 400 to 6400 international units/ml but the amount of interferon produced in the cultures did not correlate with the inhibitory effect on proliferation or with the amount of released new infectious virus. The Hall6 measles strain, originally isolated from an SSPE patient, differed most from the Edmonston strain in its characteristics, and was studied in greater detail. Although only a small amount of infectious virus was produced, the Hall6 strain had a very strong inhibiting effect on the proliferation of PHA-stimulated lymphocytes, and extensive syncytium formation was seen leading to cell death within 3 days.

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Measles virus-induced suppression of lymphocyte reactivity in vitro

Cited by 33 publications

References 12 publications

Measles Virus Persistence in Human Lymphocytes: A Role for Virus-induced Interferon

Measles Virus Persistence in Human Lymphocytes: A Role for Virus-induced Interferon

Reduced in Vitro Response of CSF Lymphocytes to Mitogen Stimulation in Multiple Sclerosis

Measles Virus Strain-dependent Variation in Outcome of Infection of Human Blood Mononuclear Cells

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