Measurement and analysis of fluorescence decay curves

Wild, Urs P.; Holzwarth, Alfred R.; Good, Hans P.

doi:10.1063/1.1134962

Cited by 105 publications

(22 citation statements)

References 13 publications

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“…With the definition of the dialysis mass transfer coefficient N , = k&C, (9) the permeability coefficient is related to the mass transfer coefficient by (10) At the concentration levels used in the present study, the second term on the righ-hand side of eq 10 is more than three orders of magnitude less than the mass transfer coefficient term and can be neglected. This also means the high flux correction of Mick1eyl5 to the Kedem- Katchalsky equations can also be neglected.…”

Section: Methodsmentioning

confidence: 99%

Transport through polymeric membranes

Collins¹,

Ramirez²

1979

J. Phys. Chem.

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Publication costs assisted by the University of ColoradoMass transport through three polymeric membranes (Cuprophan, poly(acrylonitrile), and a poly(acry1onitrile) membrane with an adsorbed protein layer) is studied. A series of eight compounds ranging in molecular weight from 60 (urea) to 1355 (vitamin BIZ) were investigated. A complete set of transport properties are reported including the permeability coefficient, sieving coefficient, pressure-filtration coefficient, and frictional coefficients representing the interactions of solute-membrane, solute-solvent, and solvent-membrane. Solute permeability for diffusive transport correlates well for all membranes studied with solute molal volume except for sulfobromophthalein (BSP). Because of strong membrane-solute frictional interactions, BSP has a reduced permeability. The sieving coefficient, which characterizes convective transport, also correlated with solute molal volume except for BSP and the lipophilic compound thiopental, The reduced convective transport of BSP is again due to its high frictional interactions with the membranes. The sieving coefficient for thiopental is markedly reduced although its permeability was normal. The membrane tortuosity factors for thiopental were also reduced. These facts indicate the existence of multiple diffusive pathways for thiopental and its exclusion from some solvent pathways in the membranes studied. IntroductionThe purpose of this study is to evaluate the mechanisms of mass transport through polymeric membranes. Membranes are important separation devices extensively used in application such as renal dialysis and reverse osmosis. Although the theory of membrane transport is well established, there are few experimental studies of membrane transport mechanisms. The work of Kauffman and Leonard7y8 and Ginzberg and Katchalsky6 are notable exceptions. In order to test theoretical concepts a wide range of solutes and two membranes often used in renal dialysis were chosen for study.Membranes are usually characterized by the following three parameters of the solute: permeability coefficient, w , the pressure filtration coefficient, L,, and the reflection coefficient, u. Often the sieving coefficient, S = 1 -u, is used instead of the reflection coefficient. The flux equations written in terms of these coefficients are (Keedem and K a t~h a l s k y )~

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Section: Methodsmentioning

confidence: 99%

Transport through polymeric membranes

Collins¹,

Ramirez²

1979

J. Phys. Chem.

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“…The instrument for the fluorescence lifetime measurement and the method of data analysis are described in detail elsewhere [11]. For the measurement of time resolved emission spectra (not deconvoluted), a reference channel system was adopted, in which the wavelength scanning was regulated so that the samples could be excited by equal number of photons at each wavelength.…”

Section: Methodsmentioning

confidence: 99%

The interaction of dansyl amino acids with bovine serum albumin

Seki

Komiyama

Iijima

et al. 1984

Colloid & Polymer Sci

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The interaction of 5-dimethylaminonaphthalene-l-sulfonyl (DNS or dansyl) amino acids with bovine serum albumin (BSA) was investigated by means of fluorescence measurements. Fluorometric titrations revealed that BSA has one high affinity site (binding constant, Ka = 105 -106 M-l), and other sites of lower affinity (K, = 103 -10 4 M-l) for the probes. Static excitation and emission spectra, lifetimes, time resolved emission spectra, and anisotropy data indicated that the bindingis stabilized mainly through fixation by the high affinity binding site. The binding constant significantly decreased with the increase of the spacer distance between the dansyl and anionic groups of the probe molecule. This observation was explained by considering the change of the electrostatic interaction between the anionic group of the probe and a cationic residue in the vicinity of the site.

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“…(i) magic angle detection, (ii) vertical excitation and vertical detection polarization, (lii) vertical excitation and horizontal detection polarization are studied. In our high repetition rate excitation experiments, population accumulation cannot be neglected [12][13][14].…”

Section: Fluorescence Decay Functionsmentioning

confidence: 99%

“…Fast photodetectors with low transit time jitter, such as side-on photomultipliers [5], end-on photomultipliers [2,6], crossed-field photomultipliers [6], microchannel plate photomultipliers [4,7], and avalanche photodiodes [8], are applied for single-photon signal detection. Powerful deconvolution techniques such as the iterative least-squares reconvolution method [l], the delta function convolution method [9,10], the instrument response mimic technique [ll], Fourier transform analysis [12,13] and the full-fit convolution method [14] have been developed to resolve fluorescence lifetimes even if they are up to a factor of ten times shorter than the instrument response time [1]. Fluorescence lifetimes down to a few picoseconds have been determined [4,7,8,15].…”

Section: Introductionmentioning

confidence: 99%

“…The iterative least-squares reconvolution method is applied. Before, only the Fourier transform analysis [12,13] and the full-lit convolution method [14] were applied to the data analysis in the case of high repetition rate excitation. The fluorescence lifetimes, molecular reorientation times, and anisotropy factors of the dyes cresyl violet perchlorate (oxa/.ine 9) and rhodamine 101 perchlorate (rhodamine 640) in ethanol are determined at room temperature.…”

Section: Introductionmentioning

confidence: 99%

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Fluorescence decay studies applying a CW femtosecond dye laser pumped ungated inverse time-correlated single photon counting system

Bäumler

Schmalzl

Goss

et al. 1992

Meas. Sci. Technol.

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An ungated inverse time-correlated single-photon counting system is described. The excitation source is a cw-pumped, linear prism-pair-stabilized, passive mode-locked femtosecond dye laser. The excitation pulse repetition rate is 120 MHz. The sample emission is detected with a microchannel plate photomultiplier tube. An iterative least-squares reconvolution method is applied to the data analysis. The fluorescence lifetimes, the reorientation times and the anisotropy factors of the dye solutions cresyl violet perchlorate and rhodamine 101 perchlorate in ethanol are determined.

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Measurement and analysis of fluorescence decay curves

Cited by 105 publications

References 13 publications

Transport through polymeric membranes

Transport through polymeric membranes

The interaction of dansyl amino acids with bovine serum albumin

Fluorescence decay studies applying a CW femtosecond dye laser pumped ungated inverse time-correlated single photon counting system

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