Estimation of Microbial Protein Supply in Ruminants Using Urinary Purine Derivatives 2004
DOI: 10.1007/978-1-4020-2844-1_20
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Measurement and Application of Purine Derivatives:Creatinine Ratio in Spot Urine Samples of Ruminants

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Cited by 26 publications
(29 citation statements)
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“…The estimation of the daily excretion obtained for each collection time was calculated by multiplying the hourly excretion by 24. Additionally, the "PDC index" (Chen et al, 2004), calculated as the ratio of the concentrations of PD to creatinine times the metabolic BW (PDC index = [PD] / [creatinine] × BW 0.75 ), was regressed on daily PD excretion to obtain a slope which represents the creatinine excretion as mmol/kg BW 0.75 (Cetinkaya et al, 2006).…”
Section: Calculation and Statistical Analysesmentioning
confidence: 99%
“…The estimation of the daily excretion obtained for each collection time was calculated by multiplying the hourly excretion by 24. Additionally, the "PDC index" (Chen et al, 2004), calculated as the ratio of the concentrations of PD to creatinine times the metabolic BW (PDC index = [PD] / [creatinine] × BW 0.75 ), was regressed on daily PD excretion to obtain a slope which represents the creatinine excretion as mmol/kg BW 0.75 (Cetinkaya et al, 2006).…”
Section: Calculation and Statistical Analysesmentioning
confidence: 99%
“…The differences in the PD/C ratio observed among experiments in the literature can be attributed to the different methodologies used. While Bargo et al (2002) and Delahoy et al (2003) used a colorimetric technique to determine allantoin, uric acid and creatinine, in our experiments, as well as in Sayers et al (2003), a HPLC was used, so higher precision and accuracy (Chen et al 2004) could be expected. In addition, in these last two studies the differences in PD/C ratio observed agree with Chen et al (2004), who reported that spot urine samples do not allow to make comparisons between experiments due animal and dietary variations; therefore they are only useful to show differences between treatments within experiments.…”
Section: Discussionmentioning
confidence: 99%
“…Analytes were detected by an ultraviolet spectrophotometer at 205 nm (Ballcels et al 1991). Microbial protein synthesis (MP) was estimated by total PD/C ratio in urine samples, as described by Chen et al (2004), and by using cattle equations reported in the IAEA (1997) manual.…”
Section: Methodsmentioning
confidence: 99%
“…Tais evidências envolvem alterações na síntese e degradação da creatinina, as quais incluem: a reversibilidade da reação de ciclização da creatina em creatinina (LEMPERT, 1959); aumento da conversão de fosfato de creatina em creatinina por hidrólise mediada pelas condições de temperatura, pH e concentração de molibdênio no organismo (MORRISON e ENNOR, 1960); e significativa conversão enzimática de fosfato de creatina em creatinina pela enzima fosforilcreatininase (IYENGAR et al, 1985). Adicionalmente, outros processos fisiológicos mais comuns podem estar associados às mudanças na excreção final de creatinina urinária, como: aumento da síntese e deposição muscular de creatina e fosfocreatina (GREEN et al, 1996); variações na taxa de filtração glomerular e excreção urinária (CHEN et al, 2004) Como resultado do somatório de efeitos das condições experimentais sobre a variação na excreção da creatinina, consequentemente houve mudanças nas estimativas de VU.…”
Section: Discussionunclassified
“…Uma vez que as taxas de filtração glomerular e de produção de urina variam (CHEN et al, 2004;BARR et al, 2005), pode haver variação nas concentrações de creatinina na urina ao longo do dia. Conforme observado no presente estudo, a taxa de depuração da creatinina apresentou tendência de variação entre período e dietas, cuja variação pode ter ocorrido também ao longo do dia.…”
Section: Discussionunclassified