Measurement of anti-DSDNA: A comparative study of two ELISA and the crithidia assay

Wong, Kok-Kien; Lawton, J. W. M.; Cheng, Sang; Lee, S.S.; Lau, Chak Sing

doi:10.1080/00313029800169675

Cited by 7 publications

(8 citation statements)

References 10 publications

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“…The aforementioned anti-dsDNA assays (CLIFT, Farr assay, ELISA) have been extensively studied through the last years with the objective of comparison. [24][25][26][27][28]…”

Section: Elisa Assaysmentioning

confidence: 99%

Detection of Antibodies to dsDNA: An Overview of Laboratory Assays

Rouquette

Desgruelles

2006

Lupus

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This brief review is focused on different methodologies available for detection of anti-dsDNA antibodies with respect to the best adequacy between biological results of laboratory and clinical significance. A large array of assays has been developed for the measurement of anti-dsDNA. New assays continually introduced have reflected not only technical innovations to avoid difficulties of some assays, but even more with hope to correlate better with systemic lupus erythematosus (SLE), particularly with its clinical course and exacerbations. Finally, with the development of micro arrays technology new insights into the pathophysiology of autoimmune disease processes should be revealed.

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“…The aforementioned anti-dsDNA assays (CLIFT, Farr assay, ELISA) have been extensively studied through the last years with the objective of comparison. [24][25][26][27][28]…”

Section: Elisa Assaysmentioning

confidence: 99%

Detection of Antibodies to dsDNA: An Overview of Laboratory Assays

Rouquette

Desgruelles

2006

Lupus

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“…A number of studies have directly compared the performance of these assays, using replicate sera from SLE patients and healthy controls (15–39). Analysis of the data from these studies shows that overall there is a statistically significant correlation among the results obtained using the different types of assays.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, several studies assessing longitudinal determinations have found that results using different assays correlate over time (25–29). Although the results for anti‐DNA testing using the different types of assays correlate for populations, there may be substantial discrepancies in the results for individual patient sera (23, 27–39). For example, in some studies discrepant results were found for more than a third of the tested sera (e.g., results were positive in one assay yet negative in another).…”

Section: Introductionmentioning

confidence: 99%

Guidelines for immunologic laboratory testing in the rheumatic diseases: Anti‐DNA antibody tests

Kavanaugh

Solomon

2002

Arthritis & Rheumatism

197

119

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This paper is part of a series on immunologic testing guidelines. The series introduction (1) outlines the full methodology for obtaining data, grading the literature, combining the information from multiple sources, and developing recommendations. Briefly, MEDLINE and Healthstar were searched using a variety of search terms, and all relevant available literature was reviewed. All papers were critically reviewed using published standards for studies of diagnostic tests. Test use was categorized as primarily diagnostic or prognostic (which also included monitoring). Information was extracted from each paper to allow for calculation of a weighted average for sensitivity and specificity; likelihood ratios (LRs) were then derived from these values (positive LR ϭ sensitivity/[1 Ϫ specificity]; negative LR ϭ [1 Ϫ sensitivity]/specificity). Recommendations for use of tests were based on the LRs, where a test was considered to be "very useful" for a given disease if the weighted average positive LR was Ͼ5 or negative LR was Ͻ0.2. A test was considered "useful" if the weighted average positive LR was Ͼ2 and Յ5 or negative LR was Ͼ0.2 and Յ0.5. A test was considered "not useful" if the positive LR was Յ2 or the negative LR was Ͼ0.5.Antibodies capable of binding double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), Z-DNA, and various other proteins associated with DNA (e.g., histones) can be detected in the serum of patients with systemic lupus erythematosus (SLE). In this analysis, the term anti-DNA antibody will refer only to antibodies specific for dsDNA (also referred to as native DNA). A number of studies have shown that antibodies to ssDNA (also referred to as denatured DNA) are less specific for the diagnosis of SLE than anti-dsDNA antibodies. While anti-ssDNA antibodies may be a useful research tool, they are an infrequently used clinical test, and will not be addressed further in this analysis.

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“…Medline, as técnicas Farr, CLIFT e ELISA mostraram sensibilidades variando de 31,6% a 98%, 8,2% a 93,3% e 14,7% a 95%, respectivamente, e especificidades variando de 53% a 100%, 43,8% a 100% e 29% a 100%, respectivamente. 58,[65][66][67]70,76,86,88,91,[94][95][96][97]100,101,[103][104][105][106]111,112,125,127,128,[150][151][152][153][154][155][156][157][158][159][160][161][162][163][164][165] Reações imunoenzimáticas utilizam usualmente preparações antigênicas imobilizadas sobre a superfície de plásticos, sendo o poliestireno um dos materiais mais utilizado. [123][124][125]130,151,[166][167]…”

Section: Considerando Os Resultados Dos Artigos Identificados No Publicunclassified

“…58,[65][66][67]70,76,86,88,91,[94][95][96][97]100,101,[103][104][105][106]111,112,125,127,128,[150][151][152][153][154][155][156][157][158][159][160][161][162][163][164][165] Reações imunoenzimáticas utilizam usualmente preparações antigênicas imobilizadas sobre a superfície de plásticos, sendo o poliestireno um dos materiais mais utilizado. [123][124][125]130,151,[166][167][168] O DNA apresenta pouca aderência ao poliestireno. O pré-tratamento do poliestireno com materiais com melhor aderência ao plástico e com afin...…”

Section: Considerando Os Resultados Dos Artigos Identificados No Publicunclassified

Desenvolvimento de uma técnica imunoenzimática (ELISA) quantitativa para a pesquisa de anticorpos IgG anti-DNA nativo (DNAds) no lúpus eritematoso sistêmico

Cavalcante¹

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pela confiança e paciência, pelo conhecimento compartilhado e pela oportunidade de desenvolver este trabalho. À Dra. Lisandra Akemi Suzuki, pela ajuda no desenvolvimento desse trabalho e pelas conversas bem-humoradas.Aos meus pais e irmã, por acreditar em mim e me ajudarem de todas as formas para que eu pudesse chegar até aqui.Aos meus amigos que, de maneira indireta, tornaram este caminho mais fácil e alegre. RESUMOO lúpus eritematoso sistêmico (LES) é uma doença autoimune crônica caracterizada pela produção de anticorpos direcionados a uma grande variedade de componentes da própria pessoa, incluindo antígenos intracelulares do núcleo celular. A detecção de anticorpos contra o DNA nativo (ds) é um dos critérios de classificação para o diagnóstico do LES. O presente estudo teve como objetivo desenvolver uma técnica imunoenzimática (ELISA) quantitativa para a detecção de anticorpos IgG anti-DNAds. O desempenho da técnica ELISA foi comparado com o teste de imunofluorescência indireta com Crithidia luciliae (CLIFT) como referência. Anticorpos IgG anti-DNAds foram pesquisados por ELISA e CLIFT em amostras de soros de 127 pacientes com LES, 56 pacientes com outras doenças e 37 pessoas sadias. A técnica ELISA apresentou sensibilidade de 92,9% e especificidade de 94,6%, enquanto a sensibilidade e especificidade da técnica CLIFT foram de 85,8% e 100%, respectivamente. O teste Q de Cochran foi utilizado para comparar as sensibilidades e especificidades das reações (diferenças entre os resultados foram consideradas significantes quando p ≤ 0,05). De acordo com a análise estatística, a técnica ELISA apresentou sensibilidade significativamente maior do que a obtida com a técnica CLIFT (p=0,0027), enquanto a técnica CLIFT apresentou especificidade significativamente maior do que a obtida com a técnica ELISA (p=0,0253). A técnica ELISA também pode ser utilizada para determinar a avidez dos anticorpos IgG anti-DNAds. Os índices de avidez desses anticorpos em 15 amostras de soros de pacientes com LES, selecionadas aleatoriamente, variaram de 58,5% a 96,9%. A técnica ELISA para a pesquisa de anticorpos IgG anti-DNAds desenvolvida nesse estudo apresentou ótimos resultados em termos de sensibilidade e especificidade, podendo ser útil em pesquisa e rotina diagnóstica.Palavras-chave: lúpus eritematoso sistêmico, ELISA, anticorpos anti-DNA.

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Measurement of anti-DSDNA: A comparative study of two ELISA and the crithidia assay

Cited by 7 publications

References 10 publications

Detection of Antibodies to dsDNA: An Overview of Laboratory Assays

Detection of Antibodies to dsDNA: An Overview of Laboratory Assays

Guidelines for immunologic laboratory testing in the rheumatic diseases: Anti‐DNA antibody tests

Desenvolvimento de uma técnica imunoenzimática (ELISA) quantitativa para a pesquisa de anticorpos IgG anti-DNA nativo (DNAds) no lúpus eritematoso sistêmico

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